EMAGE mouse gene expression spatial database

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EMAGE:13827

Grhl3 grainyhead-like 3 (Drosophila) ( MGI:2655333)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019594. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019594_01	euxassay_019594_02	euxassay_019594_03	euxassay_019594_04

euxassay_019594_05	euxassay_019594_06	euxassay_019594_07	euxassay_019594_08	euxassay_019594_09

euxassay_019594_10	euxassay_019594_11	euxassay_019594_12	euxassay_019594_13	euxassay_019594_14

euxassay_019594_15	euxassay_019594_16	euxassay_019594_17	euxassay_019594_18	euxassay_019594_19

euxassay_019594_20	euxassay_019594_21	euxassay_019594_22	euxassay_019594_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13827_wholemount_strong.wlz

13827_wholemount_moderate.wlz

13827_wholemount_weak.wlz

13827_wholemount_possible.wlz

13827_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13827_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pharyngo-tympanic tube

weak

regional

weak expression: see section 01 02 03 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 14 weak expression: see section 16

esophagus

moderate

regional

moderate expression: see section 13

stomach

weak

regional

weak expression: see section 03 05 06 07

oral epithelium

moderate

regional

moderate expression: see section 17 18 weak expression: see section 05 06 07 08 09 19 20

bladder

moderate

regional

moderate expression: see section 14 15 16

urethra of male

moderate

regional

moderate expression: see section 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55065
Entity Detected:	Grhl3, grainyhead-like 3 (Drosophila) ( MGI:2655333)
Sequence:	sense strand is shown >T55065 CTGGACGGCAAGATCCAGATCATCCTGAAGGAGCTATGAGGGCCCGGCCTCAAGCGTCCCACACCCGGGG CCCGGCTCAAGCCACGTACAACCTCTTCTGTGTCAGCTGTTACTTGAAATGCCTTTCTTTGGGAAAGAGG TCTCGCAAGCAACCAACTCGGTGATGTCCAAGCCAGGGAGAGACCAAGAAGGTTCCAGGATCTAAATGTC CCACCCAGGCTCGAACTCACTCCAGAGCTTCCTGAAAGCACCCAGCCCACCGGAGAGTCTGAGCAACACA GACCCAACTGCCTGCTTTCTCTTCTAAGTCCCGCTGCAGAGGCCCTTACAGGGGACGGGGGTCACACCAC CTTCTCTGCAGGGCTACACCCGCTGTCTCGATCGGTTCTGACGTTCACTGTTTCCTTTCTACCAACTTCA GACCAGAGAGTTCTCACACTTTGGCCAAATAACTTGAAAACTCGTGACTTTCACAGCAGATGCCTTTGTG AGGCCCTTGGAGAGGAAACTTTCTTATTGACTTCCTCGGCACAAGATGTAAGTCACCATCATCGAGCTGA CAGGAACAAATACCCTTGCCACCTACTGTTGTACACATTTCTTATTTACAGTTTTCATTATGTGATTATA TATATATATATGTAAGTATATATTATGTACATATATGCAACATTTTGTATGTCCATGTTACATTTTTATC ATTTCAAAAATATGTATTTCATATTTCTTGAACTATTTTTTTAGCTGTTATTCGATTATGCATTTTGTAT ATCATAGGGTTTAGTAATAAAAGCCTACCCATGCACACTTAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:CTGGACGGCAAGATCCAGA; Reverse Primer - name:unspecified, sequence:TTAAGTGTGCATGGGTAGGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13828 same embryo
	EMAGE:13826 same embryo
	EMAGE:13824 same embryo
	EMAGE:13825 same embryo
	EurExpress:euxassay_019594 same experiment
	MGI:4825224 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source