EMAGE mouse gene expression spatial database

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EMAGE:13839

Onecut3 one cut domain, family member 3 ( MGI:1891409)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019619. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019619_01	euxassay_019619_02	euxassay_019619_03	euxassay_019619_04

euxassay_019619_05	euxassay_019619_06	euxassay_019619_07	euxassay_019619_08	euxassay_019619_09

euxassay_019619_10	euxassay_019619_11	euxassay_019619_12	euxassay_019619_13	euxassay_019619_14

euxassay_019619_15	euxassay_019619_16	euxassay_019619_17	euxassay_019619_18	euxassay_019619_19

euxassay_019619_20	euxassay_019619_21	euxassay_019619_22	euxassay_019619_23	euxassay_019619_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13839_wholemount_strong.wlz

13839_wholemount_moderate.wlz

13839_wholemount_weak.wlz

13839_wholemount_possible.wlz

13839_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13839_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 17 18 moderate expression: see section 16

telencephalon

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24 moderate expression: see section 16

hindbrain

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 moderate expression: see section 16

midbrain

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 18 moderate expression: see section 16

facial vii ganglion

strong

regional

strong expression: see section 04 05 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 18 19

spinal cord

strong

regional

strong expression: see section 09 10 11 12 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 17 18 moderate expression: see section 16

neural retina

strong

regional

strong expression: see section 01 02 03 24 moderate expression: see section 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55136
Entity Detected:	Onecut3, one cut domain, family member 3 ( MGI:1891409)
Sequence:	sense strand is shown >T55136 ATAGCCTGTGCCAACAATCCCCTGCCTTAACGCCGGGGAGGGAAGCTGTGGGGAGCCCCCTGTCTCCAAG GAGACAGTGGCAAATGTGAGATGAGGGCTGCCCTGTGGATGCCTCCATCCCAGCACCCCTGGCTGAGTTC ACTATAAGCACCTTGCTATGTGAAGAATGCTACACAGGGAACAAGGTCACCAGAGAGCAAAGATGACACC CCAGATCTCAGGAGAGGGACATCAGATTCTCCCAGATCTGGGGGAGCCATGAAGGCACCATCGGTGTCCC TTGGATGCAAAGTTCTGGAAATCTCTGGAATTCATCACCCATTTTTCAATTGATTGGGGTGCAAACCTGA GCGCCCCTTCAGAGGACATTCAGGGGTGAGCCTTGCATTGGTGACACCTCTCTCAGAAGAGAGACGATGA AGCTGAAAACTGCTTGCTCCATTGTTTTGCCAAAAAAAAAAATGTACAATGGGGAAAGGTCAGACACGAC GGGGTCACCCCTCTGCTCAGCCCTCCAGTCTGGGTGACATTTCAAGACTGGCCTGGCAGCCATGTCCCAG GAGGCTGCCTCTTCCCCTCCCCCTCTGGGGTTCAAGATCCTGGGAATCCCTCTCCATTGAACCCTACTCC CATGGCTGCCTCCCAGAGAGGCCCCCCCACTCCCAATTTCCCTAGAGAATGCAAGAGAACTTTCTAGTGA GAAAAAGAAAAAGGAACCTTCCCCATCCTGTAAAGGCTACCTCATTGAGCCCACGCTGTGATTCGAAAGA TCACGTTTGATACCAAAGATTTCCCCACCCCTAAGGGTTTGTGCTGGAAGCCAGGGTGTCCCCACTTTCA CCCCATGGCTGTCCCCCACCCTAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGACAGGA GGACAGCGAGGTCGGCGAGTGCCTTTTTACTGAACCAAAGATGTGTGTAGAGTGTCCTCTGTCCTCAGTG ATTTGCTGCTCTCCCACAGGAGCAGCCAGGATGGATGCTTCTAGAACCTTCGGGTGTGAGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:ATAGCCTGTGCCAACAATCC; Reverse Primer - name:unspecified, sequence:CCTCACACCCGAAGGTTCTA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13836 same embryo
	EMAGE:13838 same embryo
	EMAGE:13835 same embryo
	EMAGE:13837 same embryo
	EurExpress:euxassay_019619 same experiment
	MGI:4826974 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source