EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13853

Tbx1 T-box 1 ( MGI:98493)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019629. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019629_01	euxassay_019629_02	euxassay_019629_03	euxassay_019629_04

euxassay_019629_05	euxassay_019629_06	euxassay_019629_07	euxassay_019629_08	euxassay_019629_09

euxassay_019629_10	euxassay_019629_11	euxassay_019629_12	euxassay_019629_13	euxassay_019629_14

euxassay_019629_15	euxassay_019629_16	euxassay_019629_17	euxassay_019629_18	euxassay_019629_19

euxassay_019629_20	euxassay_019629_21	euxassay_019629_22	euxassay_019629_23	euxassay_019629_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13853_wholemount_strong.wlz

13853_wholemount_moderate.wlz

13853_wholemount_weak.wlz

13853_wholemount_possible.wlz

13853_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13853_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 05 06 weak expression: see section 07 08 21 22 23 24

lower jaw incisor

weak

regional

weak expression: see section 11 16 17

lower jaw molar

weak

regional

weak expression: see section 07 20

upper jaw incisor

weak

regional

weak expression: see section 16 17

upper jaw molar

moderate

regional

moderate expression: see section 07 weak expression: see section 20

lung

weak

regional

weak expression: see section 05 06 07 08 09 10 11 15 17 18 19 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55198
Entity Detected:	Tbx1, T-box 1 ( MGI:98493)
Sequence:	sense strand is shown >T55198 TCGCTCTCGGAATCCCGTGGCTTCCCCCACGCAGCCCAATGGCTCAGACAAAGACGCTGCAGAAGCCCGG CGCGAGTTCGACCGTGACTCCGGACCCGCAGCGCTCGGCGACGCTACGCACCCGCCGCAGCTGCTGGCGC GCGTGCTGAGCCCCGCACTGCCCGGGCCTGGCGGCCTCGTCCCGCTACCCGGCGGATCCGGAGGCCGCCA CAGTCCCCCGCACGCCGATCTGCGCCTGGAGGCGCCGGGCGCGTCCGAGCCGCTGCACCACCATCCCTAC AAGTACCCGGCCGCCGCCTACGACCACTACCTCGGGGCCAAGAGCCGGCCGGCGCCCTACCCGCTGCCAG GCCTGCGCGGCCACGGCTACCACCCGCACGCGCACCCGCACGCGCACCCGCACCATCACCACCACCCCGC GGTGAACCCGGCCGCCGCCGCCGCTGCTGCCGCAGCAGCCAACGTGTACTCGTCGGCGGCCGCGCCGCCC GGTGCCTACGACTACTGCCCCAGATAGTGCGCCCGCGCGCCGACCCCGAGGGCCATCCAAGGACGCGCTC CCCATCTGGGGAGCCATGCGGGTTTCCCGCCCGCCAGTGCCAAAGCTCCCGTCCAGGCGGAAGGAAGTGG TATTTATTGTTCTCCGCGAGACCTCGTCGCCTCCGGCCCGGCCGGCAATTGCAGTGTAGACGACCGAGAG AGCCCCGCCTGCGGGCGGTGTAGATACGTGTAGATATGTGTAGATACTGTAGATACTGCACCGGCGCCGA TTTCATAAACGGTTTTGCCTCTTTTGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:TCGCTCTCGGAATCCCGTGG; Reverse Primer - name:unspecified, sequence:CCAAAAGAGGCAAAACCGTTTATGAAA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13854 same embryo
	EMAGE:13855 same embryo
	EMAGE:13857 same embryo
	EMAGE:13856 same embryo
	EurExpress:euxassay_019629 same experiment
	MGI:4828619 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source