EMAGE mouse gene expression spatial database

EMAGE:13862

Mir701 microRNA 701 ( MGI:3629658)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019276. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019276_01	euxassay_019276_02	euxassay_019276_03	euxassay_019276_04

euxassay_019276_05	euxassay_019276_06	euxassay_019276_07	euxassay_019276_08	euxassay_019276_09

euxassay_019276_10	euxassay_019276_11	euxassay_019276_12	euxassay_019276_13	euxassay_019276_14

euxassay_019276_15	euxassay_019276_16	euxassay_019276_17	euxassay_019276_18	euxassay_019276_19

euxassay_019276_20	euxassay_019276_21	euxassay_019276_22	euxassay_019276_23	euxassay_019276_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 21 weak expression: see section 07 09 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70151
Entity Detected:	Mir701, microRNA 701 ( MGI:3629658)
Sequence:	sense strand is shown >T70151 TTAGCCGCTGAAATAGATGGA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-701 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13863 same embryo
	EurExpress:euxassay_019276 same experiment
	MGI:4826370 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source