EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13872

Sp5 trans-acting transcription factor 5 ( MGI:1927715)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019631. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019631_01	euxassay_019631_02	euxassay_019631_03	euxassay_019631_04

euxassay_019631_05	euxassay_019631_06	euxassay_019631_07	euxassay_019631_08	euxassay_019631_09

euxassay_019631_10	euxassay_019631_11	euxassay_019631_12	euxassay_019631_13	euxassay_019631_14

euxassay_019631_15	euxassay_019631_16	euxassay_019631_17	euxassay_019631_18	euxassay_019631_19

euxassay_019631_20	euxassay_019631_21	euxassay_019631_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13872_wholemount_strong.wlz

13872_wholemount_moderate.wlz

13872_wholemount_weak.wlz

13872_wholemount_possible.wlz

13872_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13872_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 08 weak expression: see section 09 10 11

diencephalon lateral wall ventricular layer

weak

regional

weak expression: see section 09 10 11

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 05 06 07 08 19 weak expression: see section 09 15 16 17 18

rest of cerebellum marginal layer

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 11 13 15 16 17 19 20 weak expression: see section 10 14 18

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 13 14 15 16 17

midbrain ventricular layer

weak

regional

weak expression: see section 11 12

cochlea

moderate

regional

moderate expression: see section 08 weak expression: see section 04 05 06 07

lower jaw incisor

moderate

regional

moderate expression: see section 15 weak expression: see section 10 14

lower jaw molar

moderate

regional

moderate expression: see section 06 17 weak expression: see section 18

upper jaw incisor

moderate

regional

moderate expression: see section 15 weak expression: see section 10 11 14

upper jaw molar

moderate

regional

moderate expression: see section 06 weak expression: see section 18

lung

weak

regional

weak expression: see section 04 05 06 07 08 09 11 12 13 15 16 17 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55188
Entity Detected:	Sp5, trans-acting transcription factor 5 ( MGI:1927715)
Sequence:	sense strand is shown >T55188 AGAAGCTCAAAGTCGCTGAGGCCGGGGTGAAGCGGGAGAATCCGCGGGACCTATGAGCGCACCGGGACAC TTTCGAGGCCACTCCTGCCCAAGACATCTTTCCCAGCACCTTTGCTGGCACACCAGGGTACTTGCCATCG AGGTAGCTGACAAAGAGTAACTTTTTAAATGAACTTTTTATTCTCCTCCGCCCGAAGTCTTGCTGTCCAG CCCAAGAGCAGAGGGCAGGGCAGGCAGGACAGGAAACTGGGTCGTAGTTGAGTTACCCCAGGAGGATTCC AAAGTCCGAGCCATCGCCTGCCTGGGAGACTTACATTTTACCCAGGGCTGGCCTTGCTTGTGGGAGTCGC TGCTGAAAAAAAATTTTAAAAAGAAGGCTCTTGGGAGATTTAAAAACAAGGCCTAAGTTTTTGCTAGGCC CGATTCGGACTTTGTACAGGTTATTTAATAATAGCTTTGTTAAAGAGTAATTATGATTATAACGTTAATA AATGTTTCTGTTGTTCTCAGCTCCACGCAGAGCTACAGCATGGTACGTTTCTGTAAAGCGAACAGCAGTT GGCAGCGTGAAAATAAATACTTCATTCCAGGGTCTCCTCG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:AGAAGCTCAAAGTCGCTGAGG; Reverse Primer - name:unspecified, sequence:CGAGGAGACCCTGGAATGA. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4828396 same experiment
	EurExpress:euxassay_019631 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source