EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:13873

Brd8 bromodomain containing 8 ( MGI:1925906)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019636. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019636_01	euxassay_019636_02	euxassay_019636_03	euxassay_019636_04

euxassay_019636_05	euxassay_019636_06	euxassay_019636_07	euxassay_019636_08	euxassay_019636_09

euxassay_019636_10	euxassay_019636_11	euxassay_019636_12	euxassay_019636_13	euxassay_019636_14

euxassay_019636_15	euxassay_019636_16	euxassay_019636_17	euxassay_019636_18	euxassay_019636_19

euxassay_019636_20	euxassay_019636_21	euxassay_019636_22	euxassay_019636_23	euxassay_019636_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13873_wholemount_strong.wlz

13873_wholemount_moderate.wlz

13873_wholemount_weak.wlz

13873_wholemount_possible.wlz

13873_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13873_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

single cell

moderate expression: see section 22

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 08 09 20

trigeminal v ganglion

moderate

single cell

moderate expression: see section 06 07 08 09 10 19 20 21 22 23 24 weak expression: see section 05

dorsal root ganglion

moderate

single cell

moderate expression: see section 08 09 10 11 12 13 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T55016
Entity Detected:	Brd8, bromodomain containing 8 ( MGI:1925906)
Sequence:	sense strand is shown >T55016 GCCTTTGGTGTGTCCTTTGTGTTTGAGATGTGTTTTCTTTTTTTTTTTTTTTTTTTTTTTTAAAGACTTT ATTTATTATTATAGTAAGTACACTGTAGCTATCTTCAGACACACCAGAAGAGGGAGTCAGATCTTGTTAC GGATGGTTGTGAGCCACCATGTGGTTGCTGGGATATGAACCCTGGACCTTCGGAAGAACAGTCGGGTGCT CTTACCCACTGAGCCATCTCACCAGCCCCGAGATGTGTTTTCTTTACCATGATAACTGAGTGTGTATGGG ATACAATGGTTAGAGCTGAGTAGTCCCATAGACTGCCTGCTATGACTGAGAGGAGGTAAGTAACCAAGAC CAAAGTAGTAATTAGTGTTTTTAAAGGACTTGATTGTGTAAAAAGGACAATTTGACTATTTTACTGGGCA TTTCTTGAATGTTTGGGAAATACTAAGGGTTTTAAGATCTGGTTATATTTCTTAGGGATTAGACCACGGG CAAATGTAGCAGACAGGTCAGTCCTTGCCGATGCTGTCAACCTCTCTATAGGTAGAACTAGTGTACAGTG TTAGTTTGGTTATTTTGAGGTGGAGTCTCCCTTTTTTGATAATGCTAGCCTACAGCTCAAAATCTTTCAA TTTGAACTTTCCCAGTAGCTTGGTTTTACAGGTGTGTGTAGCACGCTAGGCCAAGAGTGCTTAAAAAAAA AAACAAACAAAATTTACTATTACATTTGTGTAAAGGTTACTCTTTGAGGGTTGTGATGTTTGGGGCTGGG AATGTTTTTGGTAGAATAATGGTACAGCTTTTGGCTAGTTTCCTTGTCAATTGCCAGTGCTGGGAGAAAG AAATCCCATGGAAACACATACCAGTAATTCCAACACTGAAAACCGAGGCAAGAGGATAACTGACAGTTTG AAGCTAGCCTGGGCTACAGAGGGAGACATTGTCTCAAAACAATCTATTAGTGGGGCCTGCATGGAGTTCT CAGTGGGT
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers. Forward Primer - name:unspecified, sequence:GCCTTTGGTGTGTCCTTTGT; Reverse Primer - name:unspecified, sequence:ACCCACTGAGAACTCCATGC. The reverse primer contains a 5' extension containing an unspecified RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using unspecified polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4823502 same experiment
	EurExpress:euxassay_019636 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source