EMAGE mouse gene expression spatial database

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EMAGE:13901

Pitpnm1 phosphatidylinositol transfer protein, membrane-associated 1 ( MGI:1197524)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007759. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007759_01	euxassay_007759_02	euxassay_007759_03	euxassay_007759_04

euxassay_007759_05	euxassay_007759_06	euxassay_007759_07	euxassay_007759_08	euxassay_007759_09

euxassay_007759_10	euxassay_007759_11	euxassay_007759_12	euxassay_007759_13	euxassay_007759_14

euxassay_007759_15	euxassay_007759_16	euxassay_007759_17	euxassay_007759_18	euxassay_007759_19

euxassay_007759_20	euxassay_007759_21	euxassay_007759_22	euxassay_007759_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13901_wholemount_strong.wlz

13901_wholemount_moderate.wlz

13901_wholemount_weak.wlz

13901_wholemount_possible.wlz

13901_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13901_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 11 12 13 14 15

brain

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 moderate expression: see section 01 02 03 04 05 06 22 23

spinal cord

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19

retina

strong

regional

strong expression: see section 01 02

neural retina

strong

regional

strong expression: see section 20 21 moderate expression: see section 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1327
Entity Detected:	Pitpnm1, phosphatidylinositol transfer protein, membrane-associated 1 ( MGI:1197524)
Sequence:	sense strand is shown >T1327 TCCTCGAGNCTGTTGGCCTACTGGCGCCGTGCAAGCACAGCCTCCTGTCCACCTGCCAGTTCTGAGGCTC CTGACGGCCCCACGAACGCTGCTCGCCTGGACTTCAAGGTCTCAGGCTTCTTCCTCTTTGGCTCCCCACT GGGCCTGGTGCTGGCTCTTCGCAAAACCGTGATGCCCGCCTTGGAGGTGGCTCAGCTGCGTCCAGCCTGC GAGCAGATCTACAATCTCTTCCATGCCGCTGACCCCTGTGCCTCCCGCCTGGAACCCCTGCTGGCCCCCA AGTTCCAGGCCATCGCCCCCCTGGCTGTACCTCGTTACCAGAAGTTTCCCCTAGGAGACGGCTCGTCCTT GCTGCTGGCTGACACTTTGCAAACCCATTCGAGTCTCTTCCTGGAAGAGCTGGAGATGATGGTGCCCTCC ACACCCACCTCGGCCAGTGGTGCCTTCTGGAAGGGCAGTGAACTAGGCAATGAGCCAGCATCCCAGACAG CAGCCCCCAGCACCACCAGCGAGGTTGTTAAGATCCTGGACCGCTGGTGGGGAAACAAGCGGATCGACTA TTCACTG
Notes:	The probe template was PCR amplified from IMAGE:2270443 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2270443 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13897 same embryo
	EMAGE:13900 same embryo
	EMAGE:13898 same embryo
	EMAGE:13899 same embryo
	EurExpress:euxassay_007759 same experiment
	MGI:4827222 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source