EMAGE mouse gene expression spatial database

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EMAGE:13919

Rb1 retinoblastoma 1 ( MGI:97874)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005526. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005526_01	euxassay_005526_02	euxassay_005526_03	euxassay_005526_04

euxassay_005526_05	euxassay_005526_06	euxassay_005526_07	euxassay_005526_08	euxassay_005526_09

euxassay_005526_10	euxassay_005526_11	euxassay_005526_12	euxassay_005526_13	euxassay_005526_14

euxassay_005526_15	euxassay_005526_16	euxassay_005526_17	euxassay_005526_18	euxassay_005526_19

euxassay_005526_20	euxassay_005526_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13919_wholemount_strong.wlz

13919_wholemount_moderate.wlz

13919_wholemount_weak.wlz

13919_wholemount_possible.wlz

13919_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13919_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

moderate

regional

moderate expression: see section 15 16 17 18 weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14

vibrissa

moderate

regional

moderate expression: see section 05 06 07 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 13 14 15 16 17

tongue

weak

regional

weak expression: see section 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2593
Entity Detected:	Rb1, retinoblastoma 1 ( MGI:97874)
Sequence:	sense strand is shown >T2593 TGGCCTCGAGCCAGATTCGGCACGAGGATCTTCCAGGGTCTTTTGAACTTGCAGTTATCTATTTTTTAAG CCAATCTGGTCTAATAACTCTGGCTTCTTCAAAGCCACACCATTTCTAGTCCAGCTGTGCAGAAACTTCA GATGAAAACAGCTGCATTGAAAATAGAGGCACTCCCTTCACCCCCCACCTAAAGGTGTATTTAAATTATC TTGTGGGATTAACTTATTTAGAGATGGTATAATTTAAAATAGGGGATATTTAAGGTAGCATCAGCTAGCA TTTAAGAAAATCACTTTTTCTAAACTCCATACTTTTTGAAAAGAAATCTGGTCTTGTTAGGAAACAAATT TCTATTTTGTCCTCAATTTAGTTTCAGTTTTACTAGTTTGATAGTTATCTCAATAACAAAAGCAATAGAC AGCTTCCCCCATTTCTTCATTAAGTTTTGCATGATCATCACACAGATTAGTTAGGTTTTAGGTCAAGGGC TTACCATACTTCTAGGTCTTTTGCTAGTGAGTTCAAGTTAGAATTAGTGACAGAATCATAGGAATTTTCA GAGATCC
Notes:	The probe template was PCR amplified from IMAGE:1383666 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1383666 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13918 same embryo
	EMAGE:13917 same embryo
	EurExpress:euxassay_005526 same experiment
	MGI:4827643 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source