EMAGE mouse gene expression spatial database

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EMAGE:13993

Keg1 kidney expressed gene 1 ( MGI:1928492)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007835. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007835_01	euxassay_007835_02	euxassay_007835_03	euxassay_007835_04

euxassay_007835_05	euxassay_007835_06	euxassay_007835_07	euxassay_007835_08	euxassay_007835_09

euxassay_007835_10	euxassay_007835_11	euxassay_007835_12	euxassay_007835_13	euxassay_007835_14

euxassay_007835_15	euxassay_007835_16	euxassay_007835_17	euxassay_007835_18	euxassay_007835_19

euxassay_007835_20	euxassay_007835_21	euxassay_007835_22	euxassay_007835_23	euxassay_007835_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13993_wholemount_strong.wlz

13993_wholemount_moderate.wlz

13993_wholemount_weak.wlz

13993_wholemount_possible.wlz

13993_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13993_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

urinary system

moderate

spotted

moderate expression: see section 04 05 20

metanephros

strong

regional

strong expression: see section 07 08 17 18 moderate expression: see section 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1407
Entity Detected:	Keg1, kidney expressed gene 1 ( MGI:1928492)
Sequence:	sense strand is shown >T1407 TCNAGCCTGTTGGCCTACTGGACTGCTTGTTCTTTGTTCTGAGGAAGCTCTCTGTGTGGATACGGAGTTA TAGGTATCAGAAGAGATGTTCTGCTTACAGAGTTCCCAAGCACTGCAGGTTCTGGAGAATTCCCTAAGGA AGCACCTCCCTGAGTCCTTAAAGGTTTATGGGACTGTCTTCCACATCAATCAGGGAAACCCATTCAAGCT CAAGACTTTGGTGGACAAATGGCCTGATTTTAATACTGTTGTTATTCGACCCCAGGAAGAGGACATGACA GATGACCTTGACCACTACAACAACACTTATCTAGTGTATTCAAAGGATCCCAAGCACTGTCAGGAATTCC TTGGCTCATCAGAAGTTATTAACTGGAAACAACATTTGCAGATTCAAAGTTCACAGTCAGACCTGGGCAA AGTGATAGAAAGTCTTGGAGCCACTAACTTGGGCAGGTCAAGCATAAGCAGTGCTTTCTCTATATGGTAT GCCAAACAGCAAAGAAACTGGCTCCTTCCTTGATGGATGCAAAGAACTTAGTAGTCAGCCGTAACAAGCT CACGCCTCTTGACCAGCAGTT
Notes:	The probe template was PCR amplified from IMAGE:2372963 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2372963 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13991 same embryo
	EMAGE:13994 same embryo
	EMAGE:13992 same embryo
	EurExpress:euxassay_007835 same experiment
	MGI:4825747 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source