EMAGE mouse gene expression spatial database

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EMAGE:13999

Ces1d carboxylesterase 1D ( MGI:2148202)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007832. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007832_01	euxassay_007832_02	euxassay_007832_03	euxassay_007832_04

euxassay_007832_05	euxassay_007832_06	euxassay_007832_07	euxassay_007832_08	euxassay_007832_09

euxassay_007832_10	euxassay_007832_11	euxassay_007832_12	euxassay_007832_13	euxassay_007832_14

euxassay_007832_15	euxassay_007832_16	euxassay_007832_17	euxassay_007832_18	euxassay_007832_19

euxassay_007832_20	euxassay_007832_21	euxassay_007832_22	euxassay_007832_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

13999_wholemount_strong.wlz

13999_wholemount_moderate.wlz

13999_wholemount_weak.wlz

13999_wholemount_possible.wlz

13999_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:13999_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

spotted

weak expression: see section 10 11 12 13

pancreas

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 12 13 14

thyroid gland

weak

regional

weak expression: see section 10 11 12 14 15

cochlear duct

moderate

regional

moderate expression: see section 07 17 weak expression: see section 05 06 08 09 16 18

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 06 07 08 09 10 12 13 14 15 16 17

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 09 10 13 14

esophagus

weak

regional

weak expression: see section 11 12 13

pharynx epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 weak expression: see section 15

stomach fundus

moderate

regional

moderate expression: see section 01 02 weak expression: see section 03 04 05 06 07

liver left lobe

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10

liver right lobe

strong

single cell

strong expression: see section 11 12 13 14 15 16 17 18 19 20

left lung

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12

right lung

strong

regional

strong expression: see section 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1337
Entity Detected:	Ces1d, carboxylesterase 1D ( MGI:2148202)
Sequence:	sense strand is shown >T1337 TCCTCGAGNCTGTTGGCCTACTGGAGAGACCCACAGAGCCCTTGTCCTTCCACAATGGGCCTCTACCCTC TGATATGGCTTTCTCTTGCTGCGTGCACAGCTTGGGGGTACCCATCCTCACCACCTGTGGTGAACACTGT TAAAGGCAAAGTCCTGGGGAAGTATGTCAATTTGGAAGGATTCACACAGCCTGTGGCTGTTTTCCTGGGA GTCCCCTTTGCCAAGCCCCCTCTTGGCTCCTTGAGATTTGCTCCACCACAGCCTGCAGAGCCCTGGAGCT TCGTGAAGAACACCACCTCCTACCCGCCTATGTGCTCTCAGGATGCTGTTGGAGGGCAGGTGCTCTCAGA GCTCTTCACCAACAGGAAGGAGAACATTCCTTTACAGTTTTCTGAAGACTGCCTCTACCTGAATATTTAC ACTCCTGCTGACTTGACAAAGAACAGCAGACTACCAGTGATGGTGTGGATCCATGGAGGTGGACTGGTGG TGGGCGGAGCATCCACCTATGATGGACTGGCCCTCTCTGCCCATGAAAATGTGGTGGTGGTGACCATTCA
Notes:	The probe template was PCR amplified from IMAGE:2286132 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2286132 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:13997 same embryo
	EMAGE:13996 same embryo
	EMAGE:13995 same embryo
	EMAGE:13998 same embryo
	EurExpress:euxassay_007832 same experiment
	MGI:4823822 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source