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EMAGE:14

Igf2 insulin-like growth factor 2 ( MGI:96434)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:14

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14EMAGE:14Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
14_voxel_strong_3D_1.wlz
14_voxel_weak_3D_1.wlz
14_voxel_possible_3D_1.wlz
14_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
perioptic mesenchyme
detected detected
regional
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:IMAGE:459226
Entity Detected:Igf2, insulin-like growth factor 2 ( MGI:96434)
Sequence:sense strand is shown

>IMAGE:459226
CAGCAGCTCCCACTTCATCCGCTCTCCCGCCCTTCTCCTCCGATCCTCCTGCGCCACGGANCCCGACCTT
CGGCCTTGTGGTACCAATGGGGATCCCAGTGGGGAAGTCGATGTTGGTGCTTCTCATCTCTTTGGCCTTC
GCCTTGTGCTGCATCGCTGCTTACGGCCCCGGAGAGACTCTGTGCGGAGGGGAGCTTGTTGACACGCTTC
AGTTTGTCTGTTCGGACCGCGGCTTCTACTTCAGCAGGCCTTCAAGCCGTGCCAACCGTCGCAGCCGTGG
CATCGTGGAAGAGTGCTGCTTCGCGCAGCTGCGACCTGGCCCTCCTGGAGACAATACTGTGCCACCCCCG
CAAAGTCCGAGAAGGACGTGTCTACCTCTCAGGCCGAACTTCCGGACGACTTCCCCAGATACCCCGTGGC
AAAGTTCTTCCAATATGACACCTGGAGACAGTCCGCGGGAGCCTGCGCAGAGGCCTGCCTGCCCTCCTGC
GTGCCCGCGGGGTCGCAATCTTGCACAAGACTTCAAGAGTCTCGAGAGCCAAACGTAACGTCCCTTGATT
CGTGTACAACCAAAGACGCGGCTACGGGTAGCCTCTTCG
Notes:Clone shows 98% nucleotide indentity over EST sequence with provisional RefSeq NM_010514.1 (Mus musculus insulin-like growth factor 2 (Igf2), mRNA).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:cryosection
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Catherine Thut, David M. Kingsley
Principal investigator:David M. Kingsley, Department of Developmental Biology, Howard Hughes Medical Institute Stanford University School of Medicine 279 Campus Drive Beckman Center B300, Stanford, USA California 94305-5329
Submitted by:Catherine Thut, Department of Developmental Biology, Howard Hughes Medical Institute Stanford University School of Medicine 279 Campus Drive Beckman Center B300, Stanford, USA California 94305-5329
Experiment type:screen
References:[ doi:10.1006/dbio.2000.0140] [ PMID:11180952] Thut CJ, Rountree RB, Hwa M, Kingsley DM 2001 A large-scale in situ screen provides molecular evidence for the induction of eye anterior segment structures by the developing lens. Dev Biol (231):63-76
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE