EMAGE mouse gene expression spatial database

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EMAGE:14033

Sh3bp4 SH3-domain binding protein 4 ( MGI:2138297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001751. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001751_01	euxassay_001751_02	euxassay_001751_03	euxassay_001751_04

euxassay_001751_05	euxassay_001751_06	euxassay_001751_07	euxassay_001751_08	euxassay_001751_09

euxassay_001751_10	euxassay_001751_11	euxassay_001751_12	euxassay_001751_13	euxassay_001751_14

euxassay_001751_15	euxassay_001751_16	euxassay_001751_17	euxassay_001751_18	euxassay_001751_19

euxassay_001751_20	euxassay_001751_21	euxassay_001751_22	euxassay_001751_23	euxassay_001751_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14033_wholemount_strong.wlz

14033_wholemount_moderate.wlz

14033_wholemount_weak.wlz

14033_wholemount_possible.wlz

14033_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14033_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 weak expression: see section 09 11 15 16 17 18 19 20

olfactory cortex marginal layer

weak

regional

weak expression: see section 11 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2737
Entity Detected:	Sh3bp4, SH3-domain binding protein 4 ( MGI:2138297)
Sequence:	sense strand is shown >T2737 GNCTGGNACAAGATGAAGAACCCCATCACCAGGCGCTGGAAGCACCTCACAGGGACACTGATCCTGGTGA ACTCACTGGACATCCTGAGGGCAGCTGCCTTCAGTCCCGCGGACCATGATGACTTTGTGATCTGAGTGGG ATCCCTGCCCTACTGCTCACTGGAGTTGGGACTCTGCCCACCAGACCTGGGACTCCACCCACCAGAGCTT GGGACTCCACCCACCAGAGCTGGGACTTGGCCCATCATCTCTCCACAGTGGGCTGTGCCATCACCGGGTG GTGCAGGGAGGGATGGCTGCTCAGGTCGAGGCCTACCTGCCAGCCACGCCCAGGAGGGACTGAGGACTTT TGACTTTCTTCCCCTCCCCTCTATCCAGGGGCAGGTCCCAGGGGCTTTCTACTTCCAGTTGCCAATTGTG TTGCTTTCTATTTGTTGAAAATGTACGTTTAAATTTAAAATCACTTTTTTAAACAGAAGACTTAGAAAGG ACGTAAGAAAGGNGGTATCTAATTTTTTAATGGACTGTAAAGGNTATTTAAAAATTTT
Notes:	The probe template was PCR amplified from IMAGE:1513631 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1513631 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14035 same embryo
	EMAGE:14036 same embryo
	EMAGE:14034 same embryo
	EurExpress:euxassay_001751 same experiment
	MGI:4828037 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source