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EMAGE:1406

Otx1 orthodenticle homolog 1 (Drosophila) ( MGI:97450)
TS16 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1406
0133d1.jpg This embryo was treated with ProteinaseK for either 3min or 30min.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1406Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1406_wholemount_strong_3D_1.wlz
1406_wholemount_moderate_3D_1.wlz
1406_wholemount_weak_3D_1.wlz
1406_wholemount_possible_3D_1.wlz
1406_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1406_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
Expression was detected in dorsal and ventral telencephalon.
diencephalon
detected detected
Expression was detected in dorsal and ventral diencephalon.
future midbrain
detected detected
Expression was detected in dorsal and ventral mesencephalon.
optic cup
detected detected
The tissue was optic vesicle.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506016
Entity Detected:Otx1, orthodenticle homolog 1 (Drosophila) ( MGI:97450)
Sequence:sense strand is shown

>MGI:3506016
CACCTTCACGCGCTCACAGCTGGACGTGCTCGAGGCGCTGTTCGCAAAGACTCGCTACCCAGACATCTTC
ATGCGCGAGGAGGTGGCACTCAAGATCAACCTGCCAGAGTCCAGAGTCCAGGTTTGGTTCAAGAACCGCC
GCGCCAAGTGCCGCCAGCAGCAGCAGAGCGGGAATGGAACGAAAACCCGGCCGGTCAAGAAGAAGTCGTC
TCCAGTACGCGAGAGCTCGGGTTCAGAGAGCAGCGGCCAGTTCACGCCGCCCGCCGTATCTAGCTCTGCT
TCTTCGTCTAGCTCAGCGTCCAGTGCCTCCGCCAACCCCGCGGCTGCTGCGGCCGCGGGCCTGGGTGGGA
ACCCAGTGGCAGCAGCGTCCTCTCTGAGCACGCCTACTGCTTCGTCCATCTGGAGCCCGGCCTCCATCTC
GCCGGGCTCAGCGCCGACATCCGTATCGGTGCCAGAGCCACTGGCCGCTCCGAGCAACGCCTCGTGCATG
CAGCGCTCGGTAGCCGCAGGTGCGGCCACTGCCGCAGCCTCCTACCCTATGTCCTATGGCCAGGGCGGAA
GCTATGGTCAGGGATACCCCGCGCCCTCCTCTTCTTACTTTGGCGGTGTAGACTGCAGCTCCTACCTAGC
GCCCATGCACTCTCATCACCACCCGCACCAGCTTAGCCCCATGGCACCCTCCTCCATGGCTGGCCATCAT
CACCATCACCCTCATGCGCACCACCCACTGAGCCAATCTTCAGGCCACCACCACCACCATCACCACCACC
A
nt 129 - nt 899 of NM_011023.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: ccggaattcaccttcacgcgctcacagctgg 3'primer: cccaagcttgtggtggtggtgatggtggtgg. Editors Note: Original information from the authors states that these two primers were used to generate a 770bp template fragment (nt273-1043 in NM_011023). Pairwise comparisons of these two primer sequences against both public versions of NM_011023 identifies a 770bp fragment in both cases however the nucleotide co-ordinates are different (nt129-899 in NM_011023.1; nt374-1141 in NM_011023.2). The modified numbering is indicated above.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508729 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE