EMAGE mouse gene expression spatial database

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EMAGE:14062

Spp2 secreted phosphoprotein 2 ( MGI:1922646)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001666. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001666_01	euxassay_001666_02	euxassay_001666_03	euxassay_001666_04

euxassay_001666_05	euxassay_001666_06	euxassay_001666_07	euxassay_001666_08	euxassay_001666_09

euxassay_001666_10	euxassay_001666_11	euxassay_001666_12	euxassay_001666_13	euxassay_001666_14

euxassay_001666_15	euxassay_001666_16	euxassay_001666_17	euxassay_001666_18	euxassay_001666_19

euxassay_001666_20	euxassay_001666_21	euxassay_001666_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14062_wholemount_strong.wlz

14062_wholemount_moderate.wlz

14062_wholemount_weak.wlz

14062_wholemount_possible.wlz

14062_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14062_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4015
Entity Detected:	Spp2, secreted phosphoprotein 2 ( MGI:1922646)
Sequence:	sense strand is shown >T4015 CATCCAGAAGCCTGGAGAGAGGCCGTCTCCCTGACTCTGGGTCGCCATCCTCTCAGTATGGAGCAGGCAA TGCTGAAGACGCTGGCTTTGTTGGTGCTGGGCATGCACTACTGGTGTGCCACAGGTTTCCCGGTGTACGA CTACGACCCTTCCTCTCTGCAGGAAGCTCTCAGTGCCTCAGTGGCAAAGGTGAACTCGCAGTCCCTGAGT CCTTACCTGTTTCGGGCGACCCGGAGCTCCTTGAAGAGAGTCAACGTCCTGGATGAAGACACATTGGTCA TGAACTTAGAGTTCAGTGTTCAGGAAACCACATGCCTGAGAGATTCTGGTGATCCCTCCACCTGTGCCTT CCAAAGGGGCTACTCTGTGCCAACAGCTGCTTTCAGGAGCACTGTGCAGATGTCCAAGGGACAGGTAAAG GATGTGTGGGCTCACTGCCGCTGGGCGTCCTCATCTGAGTCCAACAGCAGTGAGGAGATGATGTTTGGGG ACATGGCAAGATCCCACAGACGAAGAAATGATTATCTACTTGGTTTTCTTTCTGATGAATCCAGAAGTGA ACAATTCCGTGACCGGTCACTTGAAATCATGAGGAGGGGACAGCCTCCCGCCCATAGAAGGTTCCTGAAC CTCCATCGCAGAGCAAGAGTAAATTCTGGCTTTGAGTGACATCCTGGAGATTTCATGAAAGAAAGAGAAG CAGAAGCTGAAATGAAGAAAGGCATGGAGAATGGTGTCTTTTTCCTTTTTATAATCTCCACTCTGCAATA AAGATCTTTCCCTTCCTTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1433655 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1433655 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14060 same embryo
	EMAGE:14061 same embryo
	EMAGE:14059 same embryo
	EMAGE:14064 same embryo
	EMAGE:14063 same embryo
	EurExpress:euxassay_001666 same experiment
	MGI:4828424 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source