EMAGE mouse gene expression spatial database

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EMAGE:14065

Sdpr serum deprivation response ( MGI:99513)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001750. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001750_01	euxassay_001750_02	euxassay_001750_03	euxassay_001750_04

euxassay_001750_05	euxassay_001750_06	euxassay_001750_07	euxassay_001750_08	euxassay_001750_09

euxassay_001750_10	euxassay_001750_11	euxassay_001750_12	euxassay_001750_13	euxassay_001750_14

euxassay_001750_15	euxassay_001750_16	euxassay_001750_17	euxassay_001750_18	euxassay_001750_19

euxassay_001750_20	euxassay_001750_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14065_wholemount_strong.wlz

14065_wholemount_moderate.wlz

14065_wholemount_weak.wlz

14065_wholemount_possible.wlz

14065_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14065_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20

body-wall mesenchyme

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 12 13 14 15 16 17 18 19 20

tongue

weak

regional

weak expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2753
Entity Detected:	Sdpr, serum deprivation response ( MGI:99513)
Sequence:	sense strand is shown >T2753 GGCCTCGAGGCCAGATTCGGCACGAGGAANAAGATGTGCTTAAAAAGTGAACTACTAACATTTTAAAACT TATTTTGCTCTTTAACAATACTAAAGAAGTTCATATGTCTTGCAAAAATATATCTTATCTTCCCAATATT TTTATAGTCTAGTATTATAACCTAACCAGATGTTCTGATGTAAAAACTATGGTTCTTTTCACTAAATAAA TGGCAGTACACATCTTTTTGTGAAGAAGCTCAAAATAATATTGTCTCTTTAACATTGGAACAAGTGTAAG TCTCAAATAAGTAGTATGTACTGTTTTATGGGGATGTCAATCAAATGATTATTCAATATTTTTCACTTTG AAGTGACCTTTCCATTCCAAATATGATATAGGTTTAGGAGGTATTCAAACTTGCATGTTTAAGAACAGTA AAAGCCTGGGTTTTCTTTAAACACATAATGGTAATTTCAATCCAGGTAATGTTTTATTTATACATTAATT TCACAGGATATTAATGGTTTAGATTATAAAAGCATGCTAGTATTTTATTTCATGTGTACATTTGCTCTAA CATACTACTTAAAAGT
Notes:	The probe template was PCR amplified from IMAGE:1514429 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1514429 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14066 same embryo
	EMAGE:14067 same embryo
	EMAGE:14068 same embryo
	EurExpress:euxassay_001750 same experiment
	MGI:4827926 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source