EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14076

Il17d interleukin 17D ( MGI:2446510)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001743. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001743_01	euxassay_001743_02	euxassay_001743_03	euxassay_001743_04

euxassay_001743_05	euxassay_001743_06	euxassay_001743_07	euxassay_001743_08	euxassay_001743_09

euxassay_001743_10	euxassay_001743_11	euxassay_001743_12	euxassay_001743_13	euxassay_001743_14

euxassay_001743_15	euxassay_001743_16	euxassay_001743_17	euxassay_001743_18	euxassay_001743_19

euxassay_001743_20	euxassay_001743_21	euxassay_001743_22	euxassay_001743_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14076_wholemount_strong.wlz

14076_wholemount_moderate.wlz

14076_wholemount_weak.wlz

14076_wholemount_possible.wlz

14076_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14076_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 11 12 13 14 15

brain

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 17 18 19 20 weak expression: see section 21

spinal cord

weak

homogeneous

weak expression: see section 07 08 09 10 11 12 13 14 15 16

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 15 16

cervical ganglion

moderate

regional

moderate expression: see section 09 10 17 18

thoracic ganglion

moderate

regional

moderate expression: see section 10 11 12

dorsal root ganglion

strong

regional

strong expression: see section 06 07 08 09 11 12 13 14 15 16 17 18 19

nucleus pulposus

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2577
Entity Detected:	Il17d, interleukin 17D ( MGI:2446510)
Sequence:	sense strand is shown >T2577 TGGCCTCGAGGCCAGATTCGGCACGAGGTTTTTTTTTTTTTGAAGATAACACCAAAAACTTTAATCTTTA TAAAGTTAAGACCATCCATAAGCTAAAGAACAAAGGGGCGCTTGGTTCCTAGCCCCCCCACATCCCTCCC TCTCCAGGGACCTGGAGACCTTAGGTGCCCAGATCCGGAGCACTCATTATCACCTTAGTAAGCTTGGGCC ACAGGAGAGACTCAGGGACAGGGCACAGTGCAGCAGAAGGAAGTTCATCTGAGCACTACAATTATGCACT CAAGAATCCTACTAGAGGAAAACGGGATGTGTGTGATGTGCTTGTATATCAAAGAAGTTTAAAAACAACA TGCTTGCTCTAAATTACTAATATGAAAATAGTTTCTGCCACTATTCAAAATAGTTGTAGATATGTAGTGT CTACTTTGAAAAATATATTTAAAGCTTATGTAGCTGTCCTTTTGGAAAAATTAGGCAGCGGCCAGGGATC ATGGGGTGGGTTTTGTCAGGGCCAGGGGACCTGATGCATGCAGGAAGCTGGG
Notes:	The probe template was PCR amplified from IMAGE:1383201 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1383201 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14075 same embryo
	EMAGE:14078 same embryo
	EMAGE:14077 same embryo
	EMAGE:14074 same embryo
	EurExpress:euxassay_001743 same experiment
	MGI:4825566 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source