EMAGE mouse gene expression spatial database

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EMAGE:14078

Igf2r insulin-like growth factor 2 receptor ( MGI:96435)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_001742. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_001742_01	euxassay_001742_02	euxassay_001742_03	euxassay_001742_04

euxassay_001742_05	euxassay_001742_06	euxassay_001742_07	euxassay_001742_08	euxassay_001742_09

euxassay_001742_10	euxassay_001742_11	euxassay_001742_12	euxassay_001742_13	euxassay_001742_14

euxassay_001742_15	euxassay_001742_16	euxassay_001742_17	euxassay_001742_18	euxassay_001742_19

euxassay_001742_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14078_wholemount_strong.wlz

14078_wholemount_moderate.wlz

14078_wholemount_weak.wlz

14078_wholemount_possible.wlz

14078_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14078_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

visceral organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

lateral ventricle choroid plexus

weak

regional

weak expression: see section 07 08 10 12 13 14 15

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

cardiovascular system

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2614
Entity Detected:	Igf2r, insulin-like growth factor 2 receptor ( MGI:96435)
Sequence:	sense strand is shown >T2614 TGGCCTCGAGCAGATTCGGACGAGGTGGCCATATCACCACCAAGGCGGTGAAAGCAGAAGCTCTCTCGTC CCTGCATGGGGACGACCAGGACAGTGAGGATGAGGTCCTGACCGTTCCAGAGGTGAAAGTTCACTCAGGC CGAGGAGCGGAGGTGGAAAGCTCACAGCCCCTGAGAAACCCACAGCGCAAGGTCCTGAAGGAAAGGGAGG GTGAGAGGCTGGGGCTGGTCCGAGGTGAGAAGGCCAGAAAAGGGAAGTTCAGACCCGGACAGCGCAAGCC AACAGCCCCTGCCAAGCTGGTGTCCTTCCATGATGACAGCGACGAAGACCTCTTACACATCTAACTCCGT GGTGCCTACAGAAGAAGGTGGAGCCATGGACAGCCAAGCACCTCCAACCAAATAAGACTTCAGCTCGATG ATGCGTCTGTCATTTTGCCTTTAACAAAACTGCTTCAAAGGGAAGAGTTTCTGTAATGGGGGAGAGGATG GGGGAGGTGGGCACCACTCCTTCATGATCGTTTACAGTCAATGGAACAAGGTGTTGCTTG
Notes:	The probe template was PCR amplified from IMAGE:1400527 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1400527 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14075 same embryo
	EMAGE:14076 same embryo
	EMAGE:14077 same embryo
	EMAGE:14074 same embryo
	EurExpress:euxassay_001742 same experiment
	MGI:4825538 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source