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EMAGE:1409

Phox2a paired-like homeobox 2a ( MGI:106633)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1409
0134b.jpg This embryo was treated with ProteinaseK for 30min.

Expression pattern clarity: one star
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:1409Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
1409_wholemount_possible_3D_1.wlz
1409_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:1409_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
central nervous system
possible possible
Authors stated that in some instances they could not determine expression levels with certainty.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506017
Entity Detected:Phox2a, paired-like homeobox 2a ( MGI:106633)
Sequence:sense strand is shown

>MGI:3506017
CTGCAGCCAGCCTGGAGGCTTCCAATACAGTCCCCTGCGGCCTGCCTTCCCCGCCGCTGGGCCACCTTGC
CCCGCGCTCGGCTCCTCCAACTGTGCGCTTGGCGCCCTACGCGACCACCAGCCCGCACCCTACTCGGCAG
TTCCCTACAAGTTCTTCCCGGAGCCGTCCGGCCTGCATGAGAAGCGCAAGCAGCGGCGCATCCGCACAAC
GTTCACGAGTGCTCAGCTCAAGGAGTTGGAGCGCGTCTTCGCCGAGACCCACTACCCCGACATTTACACT
CGCGAGGAACTGGCGCTCAAGATCGACCTCACTGAGGCTCGCGTGCAGGTCTGGTTCCAGAACCGCCGGG
CCAAGTTCCGCAAACAGGAGCGCGCGGCCAGCGCCAAAGGCGCGGCGGGAGCGACGGGCGCCAAAAAGGG
CGAGGCGCGTTGCTCGTCGGAGGACGACGACTCCAAGGAGTCCACGTGCAGCCCCACGCCCGACAGCACC
GCGTCGCTGCCGCCGCCGCCTGCACCCAGCCTGGCCAGCCCGCGTCTGAGCCCCAGCCCTCTGCCCGCCG
CGCTGGGCTCCGGGCCCGGGCCCCAGCCGCTCAAGGGAGCGTTGTGGGCAGGGGTGGCGGGCGGTGGAGG
TGGCGGCCCCGGCACGGGCGCAGCGGAGCTGCTTAAGGCCTGGCAGCCGGCGGAACCCGGGCCAGGTCCC
TTCTCTGGAGTTCTGTCCTCCTTTCACCG
nt 105 - nt 833 of NM_008887.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: ccggaattctgcagccagcctggaggcttcc 3'primer: cccaagcttggtgaaaggaggacagaactcc
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508730 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE