EMAGE mouse gene expression spatial database

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EMAGE:14206

Rfx3 regulatory factor X, 3 (influences HLA class II expression) ( MGI:106582)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005588. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005588_01	euxassay_005588_02	euxassay_005588_03	euxassay_005588_04

euxassay_005588_05	euxassay_005588_06	euxassay_005588_07	euxassay_005588_08	euxassay_005588_09

euxassay_005588_10	euxassay_005588_11	euxassay_005588_12	euxassay_005588_13	euxassay_005588_14

euxassay_005588_15	euxassay_005588_16	euxassay_005588_17	euxassay_005588_18	euxassay_005588_19

euxassay_005588_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14206_wholemount_strong.wlz

14206_wholemount_moderate.wlz

14206_wholemount_weak.wlz

14206_wholemount_possible.wlz

14206_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14206_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4161
Entity Detected:	Rfx3, regulatory factor X, 3 (influences HLA class II expression) ( MGI:106582)
Sequence:	sense strand is shown >T4161 CAACCGTTGCCTTGTGAATCAAGCGCCATAGTCACCGTAGTCCTGGCGACTGTTACCCATCAACAACAAC TACTCCTCTCCTCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTCCCCACCACCACCATCTCCATCACCCAC CAACAACACCACAATAATCCACAGCCAAGAGACCATCATGCAGACTTCAGAGACGGGTTCAGACACAGGT TCGACAGTGACTCTGCAGACGTCTGTGGCTAGCCAAGCAGCAGTGCCTACACAGGTGGTACAGCAAGTGC CAGTGCAGCAGCAGGTGCAGCAGGTACAGACAGTTCAGCAGGTCCAACATGTCTACCCAGCTCAGGTGCA GTATGTGGAAGGAAGTGATACTGTCTATACCAATGGAGCAATCCGAACAACAACTTATCCCTACACAGAA ACACAGATGTACAGCCAAAACACTGGAGGAAATTACTTTGATACTCAAGGAAGTTCTGCCCAGGTGACAA CTGTGGTGTCCTCCCACAGTATGGTGGGTACTGGTGGGATTCAGATGGGCGTCACAGGAGGACAACTCAT CAGCAGCTCGGGAGGAACCTATTTGATCGGCAATTCAATGGAGAACTCTGGTCACTCAGTGACACACACA ACTCGGGCCTCCCCAGCAACAATTGAAATGGCGATTGAGACGCTGCAAAAGTCTGACGGTCTGTCCACTC ACAGAAGCTCTCTTCTCAACAGCCATCTGCAATGGCTGCTGGACAATTATGAGACAGCGGAGGGAGTGAG CCTGCCGAGGAGCACGCTGTACAACCACTACCTTCGACACTGCCAGGAGCACAAGCTGGACCCGGTCAAT GCCGCCTCTTTCGGAAAACTAATAAGGTCCATTTTTATGGGGCTACGAACTAGGAGATTAGGCACTAGAG GAAACTCCAAATACCATTACTATGGGATTCGTGTGAAGCCAGATTCTCCTCTTAACCGTCTACAAGAAGA TATGCAGTACATGGCTATGAGGCAGCAGCCCATGCAACAGAAGCAAAGGTACAAGCCTATGCAGAAAGTG GATGGGGTTGCAGATGGTTTCACAGGAAGTGGCCAACAGACAGGCACATCTGTTGAGCAAACTGTAATTG CCCAAAGTCAACATCATCAACAGTTTTTAGATGCATCTCGAGCACTTCCAGAGTTTGGAGAAGTTGAAAT CTCTTCTCTGCCAGATGGTACTACCTTTGAGGATATCAAGTCACTGCAGAGTCTTTATCGAGAGCACTGT GAGGCAATACTGGACGTTGTTGTGAATCTTCAGTTTAGCCTGATAGAAAAACTGTGGCAAACATTCTGGC GCTATTCCCCATCTACTCCAGCCGACGGCACTACCATCACGGAATCAAGCAATCTGAGTGAAATAGAAAG TCGACTTCCGAAAGCAAAGCTGATAACTCTGTGCAAACATGAGTCTATCCTGAAATGGATGTGTAACTGT GACCATGGGATGTACCAGGCTTTGGTGGAGATTCTCATCCCCGACGTCCTTAGACCCATTCCTAGTGCCT TGACCCAAGCCATTCGAAATTTCGCAAAAAGCCTAGAAGGTTGGCTTTCAAATGCCATGAACAATATTCC ACAGAGAATGATTCAAACCAAGGTTGCCGCTGTAAGTGCCTTTGCTCAGACTCTGCGAAGATACACATCG CTCAATCACCTGGCCCAGGCAGCCCGCGCAGTGCTTCAGAACACTTCCCAGATCAACCAGATGCTCAGTG ACCTCAACCGCGTTGACTTTGCCAATGTCCAGGAGCAGGCTTCCTGGGTGTGCCAGTGTGATGACAACAT GGTTCAGAGACTAGAAACAGACTTCAAGATGACCCTGCAGCAGCAGAGCACTCTGGAGCAGTGGGCTGCT TGGCTGGACAATGTGATGATGCAAGCGCTGAAGCCCTATGAAGGAAGGCCCAGCTTCCCGAAGGCTGCTC GGCAGTTTCTGCTCAAGTGGTCTTTCTACAGCTCCATGGTTATCCGGGACTTAACCTTGCGCAGTGCTGC TAGCTTTGGCTCCTTTCACCTGATCCGGCTGCTCTATGATGAGTACATGTTCTACTTAGTAGAGCATCGT GTTGCTCAAGTGACAGGAGAGACACCGATAGCAGTGATGGGTGAGTTTGGCGATTTAAATGCTGTATCTC CCGGAAATCTGGATAAAGATGAAGGCAGCGAAGTCGAAAGTGAGACGGATGAAGATCTGGATGATTCTTC AGAGCCTCGGGCCAAAAGGGAGAAAACGGAGCTGAGCCAGGCGTTCCCAGTAGGCTGCATGCAGCCTGTT CTGGAGAGTGCTGTGCAGCCCAGCCTCCTGAACCCCCTACACAGCGAGCACATCGTCACAAGTACACAGA CCATCAGGCAGTGCAGCGCCACAGGCAACACCTACACTGCAGTCTAACAGCAGGAAAGCAGAGTTTTCCA GCTTACTCTAACCCCATTGATACTGGGCTTAAGCTGAAATTTTAACAAGCCTGAAGGTTGACTGTTGTCA AAAGTCTATCTGTGCTGAACATTACCTTTTTGGAGTTGTGAAATGGAATGGGTGTATGTATTTTTCCAGG TCTTTTTTTTTTTTTAACACAAATTATTTGCCTTTTAATAATAGAGCCCCTCCCCCCTTAGTTTCAGGTG TCAAGAAGGATTTATACAACACTTAATGTCATTGTTTTTGTTTTCTCATAATTAAAAAGTAATTTACATT TTGTAGCTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4483833 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4483833 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14207 same embryo
	EurExpress:euxassay_005588 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source