EMAGE mouse gene expression spatial database

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EMAGE:14209

Txndc5 thioredoxin domain containing 5 ( MGI:2145316)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004021. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004021_01	euxassay_004021_02	euxassay_004021_03	euxassay_004021_04

euxassay_004021_05	euxassay_004021_06	euxassay_004021_07	euxassay_004021_08	euxassay_004021_09

euxassay_004021_10	euxassay_004021_11	euxassay_004021_12	euxassay_004021_13	euxassay_004021_14

euxassay_004021_15	euxassay_004021_16	euxassay_004021_17	euxassay_004021_18	euxassay_004021_19

euxassay_004021_20	euxassay_004021_21	euxassay_004021_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14209_wholemount_strong.wlz

14209_wholemount_moderate.wlz

14209_wholemount_weak.wlz

14209_wholemount_possible.wlz

14209_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14209_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart

moderate

regional

moderate expression: see section 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4371
Entity Detected:	Txndc5, thioredoxin domain containing 5 ( MGI:2145316)
Sequence:	sense strand is shown >T4371 AGGGGACCCGCGGCACGAGCGAGAGCTCGCCAGCCCCGCCACGATGCCCCCGCGCCCAGGACGCCTCCTC CAGCCGCTGGCCGGGCTGCCGGCCCTGGCCACGCTCCTGCTGCTGCTCGGGGCGCGCAAAGGCGCCCGGG CCCAGGAGGTGGAAGCGGACAGCGGGGTCGAGCAGGACCCGCACGCCAAGCACCTGTATACGGCCGACAT GTTCACGCACGGGATCCAGAGCGCCGCGCACTTCGTCATGTTCTTCGCGCCCTGGTGTGGACACTGCCAG CGGCTGCAGCCAACTTGGAATGACCTGGGAGACAAGTACAACAGCATGGAGGATGCCAAGGTCTACGTGG CCAAAGTGGACTGCACGGCTGATTCCGACGTGTGCTCTGCCCAGGGAGTGCGAGGATACCCCACCCTGAA GTTTTTTAAGCCTGGACAAGAAGCAGTGAAGTACCAGGGTCCTAGAGACTTTGAAACACTGGAAAACTGG ATGCTGCAGACACTGAACGAGGAGCCAGCCACACCGGAGCCGGAAGCGGAACCACCCAGAGCCCTTGAGC TCAAACAGGGGTTGTATGAGCTCTCGGCCAACAACTTTGAGCTGCATGTTTCTCAAGGCAACCACTTTAT CAAGTTCTTCGCTCCGTGGTGCGGTCACTGCAAAGCTCTGGCTCCAACCTGGGAGCAGCTGGCTCTGGGC CTTGAACATTCTGAAACCGTCAAGATTGGCAAGGTTGACTGCACGCAGCACTACGCTGTCTGCTCAGAGC ATCAGGTCAGAGGCTATCCAACTCTGCTCTGGTTTCGAGATGGCAAGAAGGTGGATCAGTACAAGGGAAA GCGGGACTTGGAGTCACTGAGAGACTATGTGCAGTCCCAGCAGCAGGGTTCAGAGGCAGCTCCGGAGACT GTTGAGCCGTCAGAGGCCCCAGTGATGGCTGCTGAGCCCACGGGTGACAAGGGCACTGTGCTGGCACTCA CCGAGAAGAGCTTCGAGGACACTATTGCACAGGGGATAACCTTCGTCAAGTTCTATGCTCCGTGGTGTGG CCACTGTAAGAATCTGGCTCCTACCTGGGAGGAGCTCTCTAAAAAGGAATTCCCAGGCTTGTCAGATGTC ACCATCGCAGAAGTGGACTGCACCGCTGAGCGCAATGTCTGCAGCAAGTACTCGGTACGAGGTTATCCCA CGTTGCTGCTTTTCCGAGGAGGTGAAAAAGTGGGAGAACACAACGGAGGTAGAGACCTCGACTCCTTACA CAGCTTTGTTCTGCGCCAGGCAAAGGATGAACTATAGGAGACCCCGGGGAAGTCATCCAGCCTGGGCTTA GGCGTGACCTCACACAGACACACGTGTTTTCACTGTGGTTAGTCAGAAAGCTGAATGTATTGAGCTTGTG TTGCTTCCTGTGTGCTCTCTGAGCCACCACACACTACGGACCTTAAGTTTCTCTAATCATGGCTCACAGT CCCTGGGCAAACCAAACGTTTTCAGTGGCGATCCATCCTGTAACCCTTCTCTTGACAGAATTAAATTGGC TCCCTTTGCAACTTAAAATACAGTCAAGGAAAGCCAAATTGCTGAACTCTGCTGGCTCCCAGGGATGTAA GGACATCATCTCGCAGGAGCAATTTGTTGCATTGCCTGAGTTTTGGGAAGGAGGCCTGCGGCACTGTTGA CTTAGTAAGGTGTCGGTCCATAGTGTACAGATTTCAAAACAAAAGCCCACAGAGCCCTGGAGATCCCTTC ATAGCCACTGCTGGGTTCCTGGGTCTCATTACTTCTCTAGTGGAGAGAGGTAAGCCATAACAAATGGTAC TTCTGATGTGTGCCCCAAATACTCATGCTGTTCAAAGGATCCTCTTGTCTGCTTCGCATAGGGTGGAGTG TGGAGATGTACGTCTTCTGCAGAAAGGGAGGGTATGGGATGCACCACAAGGTGGGTCCTCACCCGATAGG CTTTCCCAGGTCCAAGGACCAGGGAGTCTCTGCAGCTCCTCTCGGGCCTTCCTAGAAACAGCTTCTGATT CTCTTCCTAAGTAGTTCTTTTAGCAGCTCGCTTTATAATCTCCACCTTAAAGGAGGCTGACCATCTCTCT AATGATTGAGCAGTGGGATTCATCTGACCTCCAGACCAAAGACTGCAGTCGGCAGGGCTGCCCTTGCCTA GGAAGCCCAAGGTTGGATATCGCATGGCTACTTCCGGTTCCCGCCTTCCAGGTTATTAGACGCCGCTGCT CGTAACTCTGTGAAGATGCAGCCCACGTCATGCCTCTGTCTGTCTTGTTCTCAGAACCACTGTGTTTGTG CCCAGGCCCTTCACCTACCTCCCACGAGCGGGGTGAAATCAGCACCTGGCACAAGACATGTAGCAAGGAG ACTTGAAACCAGACCACGTCTGCTTCCAAAACATCAGGAGCAGAGGCGCCCCGCCAGCCTGTTATGAACG TTTATTATTAATCACGTGTGGGTCACAGCTGTCCTTGGAAACCAGTTCTGTTGGCCCAGCTCACAGTGGC TTCAGCTTAGCATCATGGTCTTATATGAAAAGAAAACTCAAGTGGTACAATTTGGTTTATACTTTCTAAA TAAACTTTTTTTTTTTTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4911455 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4911455 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14210 same embryo
	EMAGE:14208 same embryo
	EMAGE:14212 same embryo
	EMAGE:14211 same embryo
	EurExpress:euxassay_004021 same experiment
	MGI:4829032 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source