EMAGE mouse gene expression spatial database

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EMAGE:14211

Whsc2 Wolf-Hirschhorn syndrome candidate 2 (human) ( MGI:1346098)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005626. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005626_01	euxassay_005626_02	euxassay_005626_03	euxassay_005626_04

euxassay_005626_05	euxassay_005626_06	euxassay_005626_07	euxassay_005626_08	euxassay_005626_09

euxassay_005626_10	euxassay_005626_11	euxassay_005626_12	euxassay_005626_13	euxassay_005626_14

euxassay_005626_15	euxassay_005626_16	euxassay_005626_17	euxassay_005626_18	euxassay_005626_19

euxassay_005626_20	euxassay_005626_21	euxassay_005626_22	euxassay_005626_23	euxassay_005626_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14211_wholemount_strong.wlz

14211_wholemount_moderate.wlz

14211_wholemount_weak.wlz

14211_wholemount_possible.wlz

14211_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14211_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4378
Entity Detected:	Whsc2, Wolf-Hirschhorn syndrome candidate 2 (human) ( MGI:1346098)
Sequence:	sense strand is shown >T4378 CCACGCGTCCGCCCACGCGTCCGGATGGCTTCCATGAGGGAGAGTGACACCGGCCTGTGGCTGCACAACA AGCTGGGCGCCACGGACGAGCTGTGGGCGCCACCCAGCATCGCGTCCCTGCTCACCGCCGCGGTCATCGA TAACATCCGCCTCTGCTTCCACCGCCTCTCCTCGGCCGTGAAGCTCAAGCTACTGCTCGGGACGCTGCAT CTCCCGCGCCGCACCGTGGACGAGATGAAGGCGGCTCTGATGGATATCATCCAGCTGGCCACGCTGGACT CAGACCCCTGGGTCCTCATGGTTGCTGACATTCTGAAGTCCTTCCCTGACACGGGCTCCTTGAATCTGGA CCTTGAGGAACAGAACCCCAATGTTCAAGACATCTTAGGAGAACTTAGAGAAAAAGTGAGTGAGTGCGAG GCATCTGCCATGCTACCACTGGAGTGCCAGTACCTGAACAAGAATGCCCTGACCACCCTTGCTGGGCCCC TCACCCCACCAGTCAAGCATTTTCAGCTAAAGCGGAAACCTAAGAGTGCCACGCTGCGGGCAGAACTGCT GCAGAAATCTACCGAGACAGCCCAGCAGCTGAAGAGAAGTGCTGGGGTCCCATTCCACGCTAAGGGCCGG GGGCTGCTTCGAAAGATGGACACTACAACCCCTCTCAAAGGCATTCCGAAGCAAGCCCCCTTCCGAAGTC CCACCACACCCAGCGTCTTCAGTCCTTCTGGGAACCGGACTCCAATACCACCTTCGAGGACGCCACTGCA GAAAGAAAGGGGTGTGAAGCTGCTGGATATTTCTGAGCTGAATACAGTTGGTGCTGGACGAGAGGCAAAG AGGAGGAGGAAGACTTTAGACACAGAAGTGGTAGAAAAGCCCACCAAGGAAGAGACGGTTGTTGAAAACG CTACCCCTGACTATGCTGCTGGCCTGGTATCCACACAGAAACTTGGGTCTCTGAACAGTGAGCCAACACT GCCCTCCACAAGTTACCTGCCCTCTACACCCAGTGTGGTACCTGCCTCATCTTACATCCCCAGCTCTGAG ACTCCCCCAGCTCCACCCTCTCGGGAAGCCAGCCGGCCACCTGAGGAACCCAGCGCTCCAAGTCCCACAC TGCCAACACAGTTTAAACAAAGGGCACCTATGTATAACAGTGGCCTGAGCCCAGCTACACCAGCACCTGC AGCACCTACCTCACCTCTGACACCTACTACTCCCCCAGCTGTCACTCCTACTGCTCAGACACCTCCAGTG GCCATGGTGGCCCCTCAGACCCAGGCTCCAGCCCCTGTTCAGCAGCAACCTAAAAAGAACCTGTCCCTCA CTAGAGAACAGATGTTTGCTGCCCAGGAGATGTTCAAGACGGCAAACAAGGTCACTCGGCCGGAGAAGGC CCTCATCCTGGGCTTCATGGCTGGCTCTCGAGAGAACCCATGCCCGGAGCAGGGGGATGTGATCCAGATC AAGCTCAGTGAACACACAGAGGATCTGCCCAAGGCAGACGGCCAGGGCAGTACCACCATGCTAGTGGACA CAGTGTTCGAGATGAACTACGCCACAGGCCAGTGGACACGCTTCAAGAAGTACAAGCCTATGACCAATGT GTCCTAAGTGCCATCTCTGCTGCTGACCCTCATCAGACCAGAGGACAAGGTTTGCTGGTTGTGCTGAGTG ACCTGAGCAGATACCGAAGGATGTGCTCCACCCAGCACCTGTGATCCACAGGCTCCTGGGCCCAGCCATT TCTCTGGAGAGTTTAGGCTTATTTTTTATATTCTAATGAGTTAGTTTTCTGTGTGATTAAGATACTTTTT TTTATTCAATATTATGTTTTGAATGCAAACCAAAAGTTACTTCAAAATTTTAGTGCCTGTTCGACTTTTA CCTTTTTTATTTCTTCTTTAAAAAAAAAAAAAAAGTACTGGCTGAGAGGGCTTTCTTTCTCTCTCCTTGT GCATGCCCTCCCTCATGAGGTTGAGGGGACCAAAGGTGACAGCTGGAAAGTCTAGGGTGTGAGGGGGGCT ATGACCAGCCTTGATGCCACTATTTACCAGGGATTTGTATGTCTTTTGTACAGTTGTAAACATTTCAAAC CTGTGTCTGGGTTCTTATTTTCATTGTAAAATGACCTGAGTATTAAAGTTTAGTTTTTCTAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4912719 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4912719 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14210 same embryo
	EMAGE:14208 same embryo
	EMAGE:14209 same embryo
	EMAGE:14212 same embryo
	EurExpress:euxassay_005626 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source