EMAGE mouse gene expression spatial database

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EMAGE:14215

Stat3 signal transducer and activator of transcription 3 ( MGI:103038)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004039. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004039_01	euxassay_004039_02	euxassay_004039_03	euxassay_004039_04

euxassay_004039_05	euxassay_004039_06	euxassay_004039_07	euxassay_004039_08	euxassay_004039_09

euxassay_004039_10	euxassay_004039_11	euxassay_004039_12	euxassay_004039_13	euxassay_004039_14

euxassay_004039_15	euxassay_004039_16	euxassay_004039_17	euxassay_004039_18	euxassay_004039_19

euxassay_004039_20	euxassay_004039_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14215_wholemount_strong.wlz

14215_wholemount_moderate.wlz

14215_wholemount_weak.wlz

14215_wholemount_possible.wlz

14215_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14215_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forelimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07

hindlimb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4414
Entity Detected:	Stat3, signal transducer and activator of transcription 3 ( MGI:103038)
Sequence:	sense strand is shown >T4414 CCACGCGTCCGACGAGCCCTAACCGGATCGCTGAGGTACAACCCCGCTCGGTGTCGCCTGACCGCGTCGG CTAGGAGAGGCCAGGCGGCCCTCGGGAGCCCAGCAGCTCGCGCCTGGAGTCAGCGCAGGCCGGCCAGTCG GGCCTCAGCCCCGGAGACAGTCGAGACCCCTGACTGCAGCAGGATGGCTCAGTGGAACCAGCTGCAGCAG CTGGACACACGCTACCTGGAGCAGCTGCACCAGCTGTACAGCGACAGCTTCCCCATGGAGCTGCGGCAGT TCCTGGCACCTTGGATTGAGAGTCAAGACTGGGCATATGCAGCCAGCAAAGAGTCACATGCCACGTTGGT GTTTCATAATCTCTTGGGTGAAATTGACCAGCAATATAGCCGATTCCTGCAAGAGTCCAATGTCCTCTAT CAGCACAACCTTCGAAGAATCAAGCAGTTTCTGCAGAGCAGGTATCTTGAGAAGCCAATGGAAATTGCCC GGATCGTGGCCCGATGCCTGTGGGAAGAGTCTCGCCTCCTCCAGACGGCAGCCACGGCAGCCCAGCAAGG GGGCCAGGCCAACCACCCAACAGCCGCCGTAGTGACAGAGAAGCAGCAGATGTTGGAGCAGCATCTTCAG GATGTCCGGAAGCGAGTGCAGGATCTAGAACAGAAAATGAAGGTGGTGGAGAACCTCCAGGACGACTTTG ATTTCAACTACAAAACCCTCAAGAGCCAAGGAGACATGCAGGATCTGAATGGAAACAACCAGTCTGTGAC CAGACAGAAGATGCAGCAGCTGGAACAGATGCTCACAGCCCTGGACCAGATGCGGAGAAGCATTGTGAGT GAGCTGGCGGGGCTCTTGTCAGCAATGGAGTACGTGCAGAAGACACTGACTGATGAAGAGCTGGCTGACT GGAAGAGGCGGCAGCAGATCGCGTGCATCGGAGGCCCTCCCAACATCTGCCTGGACCGTCTGGAAAACTG GATAACTTCATTAGCAGAATCTCAACTTCAGACCCGCCAACAAATTAAGAAACTGGAGGAGCTGCAGCAG AAAGTGTCCTACAAGGGCGACCCTATCGTGCAGCACCGGCCCATGCTGGAGGAGAGGATCGTGGAGCTGT TCAGAAACTTAATGAAGAGTGCCTTCGTGGTGGAGCGGCAGCCCTGCATGCCCATGCACCCGGACCGGCC CTTAGTCATCAAGACTGGTGTCCAGTTTACCACGAAAGTCAGGTTGCTGGTCAAATTTCCTGAGTTGAAT TATCAGCTTAAAATTAAAGTGTGCATTGATAAAGACTCTGGGGATGTTGCTGCCCTCAGAGGGTCTCGGA AATTTAACATTCTGGGCACGAACACAAAAGTGATGAACATGGAGGAGTCTAACAACGGCAGCCTGTCTGC AGAGTTCAAGCACCTGACCCTTAGGGAGCAGAGATGTGGGAATGGAGGCCGTGCCAATTGTGATGCCTCC TTGATCGTGACTGAGGAGCTGCACCTGATCACCTTCGAGACTGAGGTGTACCACCAAGGCCTCAAGATTG ACCTAGAGACCCACTCCTTGCCAGTTGTGGTGATCTCCAACATCTGTCAGATGCCAAATGCTTGGGCATC AATCCTGTGGTATAACATGCTGACCAATAACCCCAAGAACGTGAACTTCTTCACTAAGCCGCCAATTGGA ACCTGGGACCAAGTGGCCGAGGTGCTCAGCTGGCAGTTCTCGTCCACCACCAAGCGGGGGCTGAGCATCG AGCAGCTGACAACGCTGGCTGAGAAGCTCCTAGGGCCTGGTGTGAACTACTCAGGGTGTCAGATCACATG GGCTAAATTCTGCAAAGAAAACATGGCTGGCAAGGGCTTCTCCTTCTGGGTCTGGCTAGACAATATCATC GACCTTGTGAAAAAGTATATCTTGGCCCTTTGGAATGAAGGGTACATCATGGGTTTCATCAGCAAGGAGC GGGAGCGGGCCATCCTAAGCACAAAGCCCCCGGGCACCTTCCTACTGCGCTTCAGCGAGAGCAGCAAAGA AGGAGGGGTCACTTTCACTTGGGTGGAAAAGGACATCAGTGGCAAGACCCAGATCCAGTCTGTAGAGCCA TACACCAAGCAGCAGCTGAACAACATGTCATTTGCTGAAATCATCATGGGCTATAAGATCATGGATGCGA CCAACATCCTGGTGTCTCCACTTGTCTACCTCTACCCCGACATTCCCAAGGAGGAGGCATTTGGAAAGTA CTGTAGGCCCGAGAGCCAGGAGCACCCCGAAGCCGACCCAGGTAGTGCTGCCCCGTACCTGAAGACCAAG TTCATCTGTGTGACACCATTCATTGATGCAGTTTGGAAATAACGGTGAAGGTGCTGAGCCCTCAGCAGGA GGGCAGTTTGAGTCGCTCACGTTTGACATGGATCTGACCTCGGAGTGTGCTACCTCCCCCATGTGAGGAG CTGAAACCAGAAGCTGCAGAGACGTGACTTGAGACACCTGCCCCGTGCTCCACCCCTAAGCAGCCGAACC CCATATCGTCTGAAACTCCTAACTTTGTGGTTCCAGATTTTTTTTTTTAATTTCCTACTTCTGCTATCTT TGGGCAATCTGGGCACTTTTTAAAATAGAGAAATGAGTGAGTGTGGGTGATAAACTGTTATGTAAAGAGG AGAGCACCTCTGAGTCTGGGGATGGGGCTGAGAGCAGAAGGGAGCAAGGGGAACACCTCCTGTCCTGCCC GCCTGCCCTCCTTTTTCAGCAGCTCGGGGTTGGTTGTTAGACAAGTGCCTCCTGGTGCCCATGGCATCCT GTTGCCCCACTCTGTGAGCTGATACCCCAGGCTGGGAACTCCTGGCTCTGCACTTTCAACCTTGCTAATA TCCACATAGAAGCTAGGACTAAGCCCAGAGGTTCCTCTTTAAATTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4923137 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4923137 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14216 same embryo
	EMAGE:14217 same embryo
	EMAGE:14218 same embryo
	EurExpress:euxassay_004039 same experiment
	MGI:4828485 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source