EMAGE mouse gene expression spatial database

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EMAGE:14218

March7 membrane-associated ring finger (C3HC4) 7 ( MGI:1931053)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004033. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004033_01	euxassay_004033_02	euxassay_004033_03	euxassay_004033_04

euxassay_004033_05	euxassay_004033_06	euxassay_004033_07	euxassay_004033_08	euxassay_004033_09

euxassay_004033_10	euxassay_004033_11	euxassay_004033_12	euxassay_004033_13	euxassay_004033_14

euxassay_004033_15	euxassay_004033_16	euxassay_004033_17	euxassay_004033_18	euxassay_004033_19

euxassay_004033_20	euxassay_004033_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14218_wholemount_strong.wlz

14218_wholemount_moderate.wlz

14218_wholemount_weak.wlz

14218_wholemount_possible.wlz

14218_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14218_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4449
Entity Detected:	March7, membrane-associated ring finger (C3HC4) 7 ( MGI:1931053)
Sequence:	sense strand is shown >T4449 CGGTGGAAGAGGGTCAGGGCTGCATCATTTATTGGATTGGTGTTTTTGATAAACCTATATGCTTGGTGTT TCTGATATGATTTGATATGTTCAGGTATCCCTGATTTATTTTGGAAACCTTAAGAATGGAGTCTAAACCT TCCAGGATTCCAAGAAGAATTTCTGTTCAACCCTCTGGCTCTTTAAGCACTAGGATGGTGTCTGGAAACA GAGGAACCAGTTTAAATGATTCATATCATTCTAGAGACTCCTCCTTTAGACTGGATTCTGAATATCAGTC TGCATCAGCATCAGCGTGTGCATCACCATGTCAGCCTGCCTGGTACAGTGAGTCTGAGATACCTCAGGGA GCGCGGGCACGAGCACAGACCCAGCAGCGGGATCATGACTCAAAGAGACCCAAGCTTTCCTGTACAAACT GTGCATCTACCTCAGCTGGGAGGAACGGTGGGAGTGGGTTAAATACAGTGTCAGATTCTTCTTGGAGGCA TAGTCAAGTTCCCAGATCTTCATCAATGGTACTTGGTTCATTTGGAACAGACTTGATGAGAGAAAGGAGA GATTTGGACAGGAGAAGAGAGTCCTCCATCAGCAATCTTATGGATTATAATCACCGAAGTGGTGATTTCA CAACTTCATCATATGTTCAAGAAAGAGTTCCTTCTTCATATTCACAGGGAGCAAGACCAAAAGAGAATGC AGTGAGCACTTTACAGTTGAATTCATCATCCACCAATCACCAATTGCCTTCTGACCATCAGACAGTACCA AGTTCTAGGGACTCCAGTAGAAGTTCTTTCAGATCACATTTTTCTCCAAGACAATCAGAATCTTTTCGCA ACAGTTCACATCCTGCATTTTCATATTTTTCAAGTAGAAATGAAACTCCAACTATAAGCAATTCAGAAAG GGGTTCATCTCAGAGACCATATCGAGAATCTTCTGACAATGAAGGTAGGCGTACAACTAGGAGATTGCTG TCACGGATAGCTTCTAGCATGTCATCTACTTTTTTCTCACGAAGATCTAGTCAAGATTCCTTGAATACAA GATCTTTGAGTTCTGAAAATTATATTTCTCCGAGAACCCTGACTTCACAGTCTCGGAATAATGGAACCTC CTCGTCCTCTGACGTCAGTGAGGGCAGGGCAGCTGAAGCATCTCAGGGATTTAGATTTCTTAGGCGAAGA TGGGGGTTGTCGTCGCTCAGCCAAAATCATAGCTCTGAACCAGAGGCAGAAAATTTTAACCAAGAATCAG AAGGTAGAAATTCAGGACCATGGTTGTCTTCTTCACTTAGAAATAGATGCACACCTTTGTTCTCGAGAAG GAGGCGAGAGGGAAGGGATGAGTCTTCAAGAATGTCTACGTCAGATGTACCACCTAGATCTCATATTTTC AGAAGAGATTCAAATGAAGTAGTTCATCTTGAAGCACAGGGTGACTCCCTTGGGGCTGCTGCCAACCGAC CACAAGCATCTGGAGCGTCAAGCAGTGCTGCTGCAGGTGGCTCCACCCCAGAGTTGCCTCAGGGTGGAAG AAATCCAGGACTAACAGGGATTCTTCCTGGCTCCTTGTTCCGGTTTGCAGTCCCACCAGCACTCGGCAGT AATCTGGCTGACAATGTCATGATTACTGTAGATATTATTCCTTCTGGTTGGAATTCAACTGATGGGAAAA ATGATAAAGCTAAAAGTGCACCTTCAAGAGACCCAGAAAAACTTCAGAAAATCAAAGAAAGCCTCCTTTT AGAGGACTCTGATGATGAAGAAGAAGGGGACTTATGTAGAATTTGTCAGATGGCAGCAGCGTCATCATCT AATTTATTGATAGAGCCGTGCAAATGCACAGGGAGCCTGCAGTACGTCCATCAAGAGTGTATGAAAAAGT GGTTACAAGCCAAAATTAATTCTGGCTCTTCATTAGAGGCTGTGACTACCTGTGAACTCTGTAAAGAGAA GTTGCAACTTAACCTGGAGGATTTTGATATTCATGAACTACATAGAGCTCATGCAAATGAACAAGCTGAG TATGAGTTTATCAGCTCTGGTCTCTACCTAGTTGTCTTACTGCACTTGTGTGAACAAAGCTTTTCTGATA TGATGGGAAATACAATTGAACCAAGCACTCGTGTCCGATTTATTAACCTTGCAAGAACTCTTCAGGCACA TATGGAAGATCTCGAAACTTCAGAGGATGAATTCTGAAGAAGATGGAGACCATAAGAGAATGCTTGATAT TGCCTAACTTCATTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4935124 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4935124 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14216 same embryo
	EMAGE:14217 same embryo
	EMAGE:14215 same embryo
	EurExpress:euxassay_004033 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source