EMAGE mouse gene expression spatial database

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EMAGE:14219

Tbl3 transducin (beta)-like 3 ( MGI:2384863)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004073. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004073_01	euxassay_004073_02	euxassay_004073_03	euxassay_004073_04

euxassay_004073_05	euxassay_004073_06	euxassay_004073_07	euxassay_004073_08	euxassay_004073_09

euxassay_004073_10	euxassay_004073_11	euxassay_004073_12	euxassay_004073_13	euxassay_004073_14

euxassay_004073_15	euxassay_004073_16	euxassay_004073_17	euxassay_004073_18	euxassay_004073_19

euxassay_004073_20	euxassay_004073_21	euxassay_004073_22	euxassay_004073_23	euxassay_004073_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14219_wholemount_strong.wlz

14219_wholemount_moderate.wlz

14219_wholemount_weak.wlz

14219_wholemount_possible.wlz

14219_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14219_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4124
Entity Detected:	Tbl3, transducin (beta)-like 3 ( MGI:2384863)
Sequence:	sense strand is shown >T4124 CGGGGAGAAAGGCTGCTGAACTTGGAGGCGCTGCCCTGGGACATGGCCGAGACTGCGGCGGGATTGTGCC GCTTCAAAGCCAACTATGCTGTTGAGCGAAAGATTGAACCTTTCTACAAGGGTGGGAAAGCACAGCTGGA CCAGACTGGCCATTACCTCTTCTGTGTCTGTGGTACCAAAGTTAACATCCTGGATGTGGCCTCAGGGGCT TTGCTTCGGAGCCTGGAGCAGGAGGACCAAGAGGACATTACCTCCTTTGACCTCAGTCCTGATGATGAAG TACTCGTAACAGCCAGCCGCGCACTGCTGCTGGCTCAGTGGGCCTGGCGGGAAGGCACTGTCACTCGCCT GTGGAAGGCGATACACACAGCTCCTGTGGCCTCCATGGCTTTTGATGCCACCTCTACACTGTTAGCCACA GGCGGCTGTGATGGGGCTGTACGTGTTTGGGACATTGTGCAACATTACGGGACACACCACTTTCGAGGCT CACCTGGTGTGGTGCATTTGGTGGCCTTCCATCCAGACCCCACCCGCCTGCTGCTCTTCTCCTCAGCTGT GGACACCTCTATCCGCGTGTGGTCACTGCAGGACCGCTCGTGTTTGGCTGTCCTGACTGCACACTATAGT GCTGTCACTTCTCTGTCCTTTAGTGAGGGTGGCCACACTATGCTCAGTTCTGGCCGTGACAAGATCTGCA TAGTTTGGGACCTTCAGAGCTACCAGACTACAAGGACGGTGCCAGTGTTTGAGAGCGTGGAAGCCTCTGT GCTGCTGCCCGAACAGCCGGCACCTGCGCTGGGTGTGAAGAGCTCAGGCCTGCACTTCTTAACAGCTGGT GACCAAGGGATCCTTCGTGTGTGGGAGGCTGCTTCTGGGCAGTGTGTATACACCCAGCCACAAATGCCAG GCCTACGGCAGGAACTGACCCACTGTACCCTGGCCCGTGCTGCTGACCTGCTCCTCACTGTCACTGCCGA TCACAACCTGCTCCTCTATGAGGCACACTCCTTACAGTTGCAGAAACAGTTTGCTGGCTATAGTGAGGAA GTATTGGATGTTCGCTTCCTGGGGCCCAGTGACTCCCACATCGTCGTGGCTTCCAACAGCCCTTGCCTGA AGGTGTTTGAGCTGCAGACTTTGGCCTGTCAGATCCTCCATGGTCACACAGACATTGTCTTGGCCCTGGA TGTGTTCCGGAAGGGGTGGCTCTTTGCTAGCTGTGCCAAGGATCAGAGCATACGCATCTGGAAGATGAAC AAGGCTGGCCAGGTGGCCTGTGTAGCTCAGGGTTCTGGCCACACACATAGTGTGGGCACCATCTGCTGTT CTAGGCTAAAAGAATCTTTCCTGGTGACTGGCAGTCAGGACTGTACTGTGAAGCTCTGGCCCCTCCCTGA AGCTCTGCTGGCCAACAAGAGCACAGCTGCAGACAGTGGCCCCGTGCTTCTACAAGCCCAGACCACTCAG CGATGCCACGACAAAGACATCAACAGTTTGGCTGTCTCCCCCAATGACAAGCTGTTAGCAACAGGCTCAC AGGATCGCACAGCTAAGCTCTGGGCCTTGCCTCAGTGCCAACTGTTAGGTGTTTTTACAGGCCACCGACG TGGCCTCTGGAATGTCCAGTTCTCACCTACGGACCAGGTGCTGGCCACAGCCTCTGCTGATGGTACCATC AAGCTCTGGGCACTGCAGGACTTCAGTTGTCTCAAGACATTTGAGGGACACGATGCTTCTGTACTGAAGG TGGCCTTTGTAAGCCGTGGCTCACAGCTGCTGTCTAGTGGCTCTGATGGGCTCCTGAAGCTGTGGACCAT TAAGAGCAACGAGTGTGTGCGGACCCTGGATGCTCACGAGGACAAGGTCTGGGGACTACACTGCAGCCAA CTGGATGATCATGCCATCACCGGTGGCAGTGACTCCCGAATCATTCTCTGGAAGGATGTGACAGAAGCTG AGCAGGCAGAAGAGCAGGCCAAACGGGAAGAGCAGGTGATCAAGCAGCAAGAACTGGACAACCTGCTCCA TGAGAAGCGGTATCTCCGGGCACTAGGCCTTGCCATCTCTCTGGATCGTCCCCACACTGTGCTGACCGTT ATCCAGGCCATCCGGAGAGATCCTGAGGCCTGTGAGAAGCTGGAAGCCACTGTACTCCGACTGCGAAGAG ACCAGAAAGAGGCCCTGCTGCGCTTCTGTGTCACGTGGAACACAAACTCCAGACATTGCCACGAAGCCCA GGCTGTGCTTGGTGTGCTGCTGCGGCACGAGGCTCCTGAGGAGCTGCTGGCCTACGACGGGGTGCGGGGG TCACTGGAGGCCTTGCTGCCCTACACTGAGCGGCACTTTCAGCGGCTTAGCAGGACACTGCAAGCAGCCA CCTTCTTGGACTTCCTGTGGCACAACATGAAGCTGTCCCCATGCCCTGCTGCAGTTCCCCCAGCCCTATG AAACAGAAAAGTTAACTTTCTAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4460615 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4460615 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14222 same embryo
	EMAGE:14220 same embryo
	EMAGE:14223 same embryo
	EMAGE:14221 same embryo
	EurExpress:euxassay_004073 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source