EMAGE mouse gene expression spatial database

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EMAGE:14221

Med24 mediator complex subunit 24 ( MGI:1344385)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004057. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004057_01	euxassay_004057_02	euxassay_004057_03	euxassay_004057_04

euxassay_004057_05	euxassay_004057_06	euxassay_004057_07	euxassay_004057_08	euxassay_004057_09

euxassay_004057_10	euxassay_004057_11	euxassay_004057_12	euxassay_004057_13	euxassay_004057_14

euxassay_004057_15	euxassay_004057_16	euxassay_004057_17	euxassay_004057_18	euxassay_004057_19

euxassay_004057_20	euxassay_004057_21	euxassay_004057_22	euxassay_004057_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14221_wholemount_strong.wlz

14221_wholemount_moderate.wlz

14221_wholemount_weak.wlz

14221_wholemount_possible.wlz

14221_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14221_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6143
Entity Detected:	Med24, mediator complex subunit 24 ( MGI:1344385)
Sequence:	sense strand is shown >T6143 CGGACGCGTGGGCGGACGCGTGGGTCGACCCACGCGTCCGCGGACGCGTGGGTGGCGCTGCCTACGATGT ACAACCGGTTTGTATGAACTCCAGTTCTCATCTGGCTCCGTTTTTAAACTCCCACCTTGTCACTCATCTC GCCTCCTTGGAGTGACATCATGAAGGTGGTGAACCTGAAGCAGGCCATTTTGCAAGCCTGGAAAGAGCGA TGGAGTGACTACCAATGGGCAATCAACATGAAAAAATTCTTCCCCAAAGGAGCCACCTGGGACATTCTCA ACCTAGCAGAAGCGCTCCTGGAGCAGGCTATGATTGGGCCTTCCCCCAATCCTCTGATCCTGTCGTACCT GAAGTACGCCATTAGTTCCCAGATGGTGTCCTGCTCCTCTGTGCTCACCGCCATCAGTAAGTTTGATGAC TTTTCCCGGGACCTGTGTGTCCAGGCTCTGCTGGATATCATGGACATGTTCTGTGACCGACTCAGTTGCC ACGGCAAAGCAGAGGAATGCATCGGGTTGTGCCGAGCGCTCCTTAGCGCCCTCCACTGGCTGCTTCGATG TACAGCAGCTTCTGCGGAGCGACTCCAGGAAGGGCTGGAGGCTGGCACTCCGGCCCCCGGGGAGAAGCAG CTTGCCTTGTGCTTGCAGTGCCTGGAGAAGACCCTCAGCAGCACCAAGAACCGAGCCCTGCTACACATCG CCAAACTAGAGGAGGCCTCCTCCTGGACTGCCATAGAGCATTCTCTTCTAAAACTTGGGGAGATCCTGGC CAATCTCAGCAACCCCCAGCTCCGGAGCCAAGCTGAGCGTTGCGGCACACTCATCAGGAGCATCCCCAGC ATGCTGTCCGTGCACTCCGAGCAACTGCACAAGACCGGCTTCCCCACCATCCACGCACTCATCTTGCTTG AGGGCACCATGAACCTGACCGGGGAGATGCAGCCCCTGGTGGAGCAGCTGATGATGGTGAAGCGCATGCA GCATATCCCCACACCTCTCTTTGTCCTGGAAATCTGGAAAGCTTGCTTTGTGGGACTCATTGAGTCCCCC GAGGGCACGCAGGAGCTGAAGTGGACAGCTTTCACCTACCTCAAGATTCCACAGGTCTTGGTGAAGTTGA AGAAGTACTTTCATGGAGAGAAGGATTTCACGGAGGATGTCAACTGTGCTTTTGAGTTCCTGCTGAAGCT CACCCCCTTGTTGGACAAAGCTGACCAGCGCTGCAATTGCGACTGCACGAACTTCCTCCTCCAGGAGTGC AACAAGCAGGGGCTTCTGTCTGAGGTCAACTTTGCCAGCCTTGTGGGCAAACGCACGGCAGATCGGGACC CGCAGCTAAAATCATCAGAAAATGCTAACATCCAGCCGAATCCTGGCCTGATCCTGCGGGCAGAGCCCAC GGTCACAAACATTCTCAAGACAATGGATGCAGATCACTCCAAGTCCCCGGAGGGACTGCTAGGGGTCCTG GGACATATGCTGTCTGGGAAGAGCCTGGATTTGCTGCTGGCTGCTGCCGCCGCCACTGGGAAGCTTAAAT CCTTCGCCCGGAAATTCATCAATCTGAATGAGTTCACGACCCACGGCAGTGGAGAGAGCACGAAAACAGC CTCGGTCCGAGCCTTGCTCTTTGACATTTCCTTCCTCATGCTATGCCATGTGGCCCAGACCTATGGCTCA GAGGTGATCCTCTCGGAGTCGAGCTCAGGAGAAGAAGTCCCCTTCTTTGAGACTTGGATGCAGACCTGCA TGCCCGAGGAGGGCAAGATTTTGAACCCTGACCATCCCTGCTTCCGCCCTGACTCCACCAAAGTCGAGTC CTTGGTGGCCCTGCTCAACAACTCTTCAGAGATGAAGCTAGTACAGATGAAGTGGCACGAGGCCTGCCTC AGCATTTCAGCAGCCATTTTGGAAATCCTCAACGCTTGGGAGAATGGGGTTCTGGCATTTGAGTCCATCC AGAAAATCACCGATAATATCAAGGGAAAGGTATGCAGTCTGGCGGTGTGTGCTGTGGCTTGGCTCGTGGC CCACGTCCGGATGCTGGGGCTGGATGAGCGTGAGAAGTCACTGCAGATGATCCGCCAGTTGGCAGGGCCA CTGTACAGCGAGAACACCCTGCAGTTCTACAATGAGAGGGTGGTGATTATGAACTCAATCTTGGAACACA TGTGTGCGGACGTGCTCCAGCAGACAGCCACGCAGATCAAGTTTCCATCCACAGGCGTGGACACGATGCC CTACTGGAACCTGCTTCCTCCCAAGCGCCCCATCAAGGAGGTCCTGACAGACATCTTTGCCAAGGTACTG GAGAAGGGCTGGGTGGACAGCCGCTCCATCCACATCCTTGACACCCTGCTGCATATGGGCGGCGTCTACT GGTTCTGCAACAACCTGATTAAGGAGCTGCTGAAGGAGACTCGCAAGGAGCACACGTTAAGGGCGGTCCA GCTTCTCTACTCCATCTTCTGTCTGGACATGCAGCAAGTGACCCTGGTCCTGCTGGGTCACATCCTGCCC GGGCTGCTTACTGACTCTTCCAAGTGGCACAGCCTTATGGACCCTCCTGGCACTGCGCTGGCCAAACTGG CTGTGTGGTGTGCCCTGAGCTCTTACTCTTCCCACAAGGGACAGGCGTCCTCCCGCCAGAAGAAGAGACA CAGAGAGGACATTGAGGACTATGTCAGCCTCTTCCCCGTGGAGGACATGCAGCCCTCGAAGCTGATGCGA CTCCTGAGCTCCAGTGACGATGATGCCAACATCCTGTCAAGTCCCACTGACCGCTCCATGAACAGCTCCC TCTCAGCCTCCCAGCTCCACACAGTCAACATGAGGGACCCTCTGAACCGAGTCCTGGCCAACCTGTTCCT GCTCATTTCCTCCATCTTGGGTTCACGCACCGCTGGCCCCCACACCCAGTTTGTGCAGTGGTTCATGGAG GAGTGTGTGGGCTGCCTGGAGCAGGACAGCAGGGGCAGCATTCTGCAGTTCATGCCCTTCACTACCGTAT CAGAACTGGTGAAAGTGTCTGCTATGTCCAGTCCCAAGGTTGTTCTGGCCATCACAGACCTCAGCCTGCC CCTGGGCCGGCAGGTGGCCGCCAAAGCCATTGCTGCACTCTGAGGAGCTCCGTGTGGCCTGCAGTGGGTC CCAGCCCCATCATCCAAGCAGGGAGCCAGGAGAGTGAGACCTTCCCACCCCTGCCCTGACTCATCATGGC CCTACACATCTCCCAGTGGCTTTTGTAATTTCTGTCCTTTGAACCAGCACCCAAAGACTCTGAGCAGTCC CTATGGTCACCCCAAGTAGCTGTACCTCCAGGAGGGACTTTATAGGGCCACAGTGTGTATATAAGTTTTA TTTTTATAACCCATGAATGGGGAGTCCCCCTTGGTTCCTCTGTAAATAAAGATCCTTGTGGCTGTGAAAA AAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3496140 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3496140 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14222 same embryo
	EMAGE:14220 same embryo
	EMAGE:14223 same embryo
	EMAGE:14219 same embryo
	EurExpress:euxassay_004057 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source