EMAGE mouse gene expression spatial database

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EMAGE:14223

Stxbp1 syntaxin binding protein 1 ( MGI:107363)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004040_01	euxassay_004040_02	euxassay_004040_03	euxassay_004040_04	euxassay_004040_05

euxassay_004040_06	euxassay_004040_07	euxassay_004040_08	euxassay_004040_09	euxassay_004040_10

euxassay_004040_11	euxassay_004040_12	euxassay_004040_13	euxassay_004040_14	euxassay_004040_15

euxassay_004040_16	euxassay_004040_17	euxassay_004040_18	euxassay_004040_19	euxassay_004040_20

euxassay_004040_21	euxassay_004040_22	euxassay_004040_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14223_wholemount_strong.wlz

14223_wholemount_moderate.wlz

14223_wholemount_weak.wlz

14223_wholemount_possible.wlz

14223_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14223_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4131
Entity Detected:	Stxbp1, syntaxin binding protein 1 ( MGI:107363)
Sequence:	sense strand is shown >T4131 GCGGGGCGCGCGGCCCGGGGGAGGCGACGGTGTCGCGGGAGGAGCATCGGAGCCCGAAGACTCGAAGAAC GCCATGGCCCCCATTGGCCTCAAGGCGGTGGTCGGAGAGAAGATCATGCATGATGTGATCAAGAAGGTGA AGAAGAAGGGCGAGTGGAAGGTGCTGGTGGTGGACCAGTTAAGCATGAGGATGCTGTCCTCCTGCTGCAA GATGACAGACATCATGACCGAGGGCATCACAATTGTGGAGGATATCAACAAGCGCCGAGAGCCACTCCCC AGCCTGGAGGCCGTGTACCTCATCACCCCATCTGAGAAGTCTGTCCACTCTCTGATCAGTGATTTTAAGG ACCCGCCGACTGCTAAATATCGGGCTGCGCATGTGTTCTTCACAGACTCGTGTCCAGATGCCCTATTTAA CGAGCTGGTAAAATCCCGAGCAGCCAAAGTCATCAAGACGCTGACGGAAATCAACATTGCGTTTCTCCCC TATGAGTCCCAGGTGTATTCCCTGGACTCCGCTGACTCTTTCCAAAGCTTCTACAGCCCTCACAAGGCGC AGATGAAGAATCCGATACTGGAACGCCTGGCAGAGCAGATCGCAACCCTGTGTGCCACCCTGAAGGAGTA TCCAGCTGTGCGGTATCGGGGGGAGTACAAGGACAATGCCTTGCTGGCTCAGCTGATCCAGGACAAGCTG GATGCCTATAAAGCCGACGATCCAACAATGGGGGAGGGTCCCGACAAGGCACGGTCCCAGCTCCTGATCC TGGATCGTGGCTTTGACCCCAGCTCCCCTGTGCTCCATGAACTGACATTCCAGGCTATGAGTTATGACCT GCTGCCTATCGAAAATGATGTTTACAAGTATGAGACCAGCGGCATTGGAGAGGCGCGGGTGAAGGAGGTG CTACTGGATGAGGACGATGACCTGTGGATTGCGCTGCGACACAAGCACATCGCAGAGGTGTCCCAGGAAG TCACCCGGTCTCTGAAGGACTTTTCCTCTAGCAAGAGGATGAACACTGGCGAGAAGACCACCATGCGGGA CCTGTCCCAGATGCTGAAGAAAATGCCCCAGTACCAGAAGGAGCTCAGCAAGTATTCGACTCACCTGCAC CTTGCTGAAGACTGCATGAAGCATTACCAAGGCACTGTAGACAAACTCTGCCGCGTGGAGCAGGACCTGG CAATGGGCACAGATGCTGAGGGGGAAAAAATCAAGGACCCCATGAGAGCCATTGTCCCCATCCTGCTGGA TGCGAACGTCAGCACTTACGACAAAATCCGTATCATCCTTCTCTACATCTTCCTGAAGAACGGTATCACT GAGGAGAACCTAAACAAACTCATCCAGCACGCTCAGATACCCCCAGAGGACAGCGAGATCATCACCAACA TGGCTCACCTCGGCGTGCCCATCGTCACGGATTCCACACTACGCCGCCGAAGCAAACCGGAGCGGAAGGA GCGTATCAGTGAGCAGACCTACCAGCTCTCACGATGGACCCCGATCATTAAAGACATTATGGAGGACACT ATCGAAGACAAGCTGGATACAAAGCACTACCCATACATCTCTACCCGCTCGTCCGCGTCCTTCAGCACCA CTGCTGTGAGTGCCCGCTATGGACATTGGCACAAGAATAAGGCCCCCGGGGAGTACCGCAGCGGTCCCCG CCTCATTATTTTCATCCTTGGGGGTGTGAGCCTGAATGAGATGCGCTGTGCTTACGAAGTGACCCAGGCC AACGGCAAGTGGGAAGTGCTGATAGGTTCTACTCACATTCTCACTCCCACCAAATTCCTCATGGACCTGA GACACCCCGACTTCAGGGAGTCCTCTAGGGTATCTTTTGAGGATCAGGCTCCAACAATGGAGTGAGAGCC AAAGAGACAAAGATCCACGCACATTCTCACCCCACAGAAACTGCTGGACACGCTGAAGAAGCTGAATAAA ACAGATGAAGAAATAAGCAGTTAAAAAATAAGCTGCCCCCCAAAACCCCGGCTCCCTTCCCAAAATGCTC TGCAGCTCCCCCGTGCGCCACCTCGGTTACTCTGCTGCCTCCCCAGCCCTGCACGCCCTGGCCACCCCGT TGCCGTGCTGAGTTCTTCTCCTGTGCGATGACACCCCATCTTGTCCTCTGAAAAGCAAGAGAGTAATGTG TTGTTTTTTAAAAATGAGCATCTTCTGTATGTATCCCACAGTAAGTTCACATGCAAGCTCCACACTGCAG AAGCGTCAGAACTCCGGACCGAGTGAATTCTCCCTTATTTATGACCCCGTGACCTGTATATAGCCCTGTC CCGCGTGTGCACATTGCTTGAATATGGAAAGGTAGATGTGTGGGTGTCTCTCCAAGCTTGGTTGGATTCA TTTCTGTCCTTGTTGGTGTTTGTTCCCCGGATAGGACATGCTGAGGGAGTGATGTTCTCGCTAGCCCCTG CTCGCTCCCTGTTCTCAGCGATGAGCAGACACCTCTGGAGGCTGGCGTGGAACGAGCCTCCTCTTTGCAC CTATGGGGGAGGCTTAGGGTGTCCACAGGAAGCCAGTCTGAGTGCCGGCCAGTGTGGTCTCCAGAGCCTG GCACTGCTTTCCCTGATCTGTGTCCATACTGTTGTAACAAGTTAAGCCCTTCAGGCTAAATCAGCCTGCC TAGTGCCCTCGGAGCCTCCAGAGTTAGGTCTGACCAGCCCCCTGCTTGAACACAGTTTGGATAGAGGCCA AGGGTCAGGGGTGGGCTGGAAGCTGTGAGTTTGGCACTCTGGTCAAGGGTGCTTTGCTGTAGGAGCTAGG CCTGAAGAATGGGGGCCCTGCTGCTTAGTCAGAGTCCCCTCAGTTTAAGATACTTCATCAATCTTAAGTT TGTGTAGTGTACAGTCATGTGTCATTGTGGTTGTATGAAAAGGATCATTTTATTCTTTGTATTAGTCATC ACTGTATAAAACATAGCTAGCTATAAAGCAGAAATTCCAGAAGCCGATGCTGGAAGGATGGTCTCCACCC TCAGGACGCAGCAGCCCCCTGAGCATGCTGCTCACCCTGCTGCTGTAGTCGTGAAACAAAGACAGTGGAA GTCACAAAAATGTCCCCAGCCCAGTCCCCGCCCCTCCCTCCCGCAGATTTGTACGTATTACTGTGTCTCG TGCTGTCTTCGCAAACGTGGTGTACGCCTGCCGCAGGTGTCCTGTGCCCTTCTCCCTCCCTGACTCTAGA GTCTCTCTTCTCCTTAGTTCTCAGGCCTCTCCCCTGCTCCTCTCCAGTGAACCTTTTCCCTTAGGACTGA ACCACACTAGCACCGGTTGATTTCTTCTGTAGCGCTTCTCCCATCCCTTCCTCCGGTCAAGCAATGCTCA TGCTTCAGGATCTTGTTTGTCGAACATGTGGGGTTTCCTTTATGTTATTTATATAAATAATTTCTCAAAT GGATATTTAAAAAAAAGCTAGTCTGTCTTGAAACTTGTTAACTTGAAACTCTTGAATCTCAGTGTTTAAA GTATGGAAGCACAACCGTGTACCGCTCTGTACCGTCCTGTACTGCAGCATTTGAGTCTAATAAAGACGTC AGCTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4480978 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4480978 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14222 same embryo
	EMAGE:14220 same embryo
	EMAGE:14221 same embryo
	EMAGE:14219 same embryo
	EurExpress:euxassay_004040 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source