EMAGE mouse gene expression spatial database

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EMAGE:14225

Ttc23 tetratricopeptide repeat domain 23 ( MGI:1914259)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003967. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003967_01	euxassay_003967_02	euxassay_003967_03	euxassay_003967_04

euxassay_003967_05	euxassay_003967_06	euxassay_003967_07	euxassay_003967_08	euxassay_003967_09

euxassay_003967_10	euxassay_003967_11	euxassay_003967_12	euxassay_003967_13	euxassay_003967_14

euxassay_003967_15	euxassay_003967_16	euxassay_003967_17	euxassay_003967_18	euxassay_003967_19

euxassay_003967_20	euxassay_003967_21	euxassay_003967_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14225_wholemount_strong.wlz

14225_wholemount_moderate.wlz

14225_wholemount_weak.wlz

14225_wholemount_possible.wlz

14225_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14225_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4166
Entity Detected:	Ttc23, tetratricopeptide repeat domain 23 ( MGI:1914259)
Sequence:	sense strand is shown >T4166 GTTTTGTTAAGTACCGGGTTGCTTTATGGAAAAATGCTGGGTTTTTTTTTTAGGACGAAACTGCTCTGTT TAGACTTGTCTGTGGTTATCAGGCTGTAGAATCATTAACTTGATACAGAGGGAGCCAAATTGGCAACTCA GATTTTCAAGAGAACTGTGAAAAGGAAAAGGACTCTCTCTCCATGGGACCGCTAAACTCACAAAGGATTT TACGACAAAGTTGCAGATACTGAGCCTTAAGGAAACTTTCCAGGTGTTTTGTTTTGCTAGTTTTAAAGGA ACGTCCAAAGAAAACCAGAGGGAACACCTCGTGCTGAGCGTGGCTGGAGGAAGAGGCATCAGAGCTCTTT TGTCTTTAGAATGAAGGTGGAAACGCAATTCCCAGGTTATCAGTGGAATTTTGCAAGAGGACAATGCGTA TGAGAATATGCAGGAGTCCCAGGACACCCACATGTCCAGCCACCTAGACGAAGTCGTTGCTGCTGTCAGC GTCACTTCTAAAAACAGGATCCCGAATAAGCTGCTTCAGACAGCACTGTTCCAGCCCCCTCGAGAGAAAC TCCACCTGTGTGAGGAGAGAGCAAAGTCCTACTCCAGCAGCCGAGAGTACAAACAGGCCATCCAGGAGCT TGTGCGCTGTGTGGCACTAACAAGAATTTGCTATGGCGACTGGCACTGGAAACTAGCGGAGGCGTATGTT AATCTGGCTCAGGGTTACCTGCAGCTGAAAGGGTTGTCACTACAAGCAAAGCAGCATGCAGAGAAAGCCA AGGAGATCCTGGCCAACTCCATCGAGAGTCCCTGTCACAACAAAACAGATATCTTCAAGTGTTCCCTGGA GCTTTTCTATACCCTGGGGAGAGCGCTGCTCTCCCTTCAAAAATTTAAAGACGCCTCAGAGAACTTGATA AAAGCAGAGAGACTTTCCAAGGAGATGCTGCAATGTGGGAACATTGTAAAGGAAGAGTGGATAGAAATCC AGTCTCGAATCAAGCTGTCGTTTGCACAGTTGTATCAAGGTCAGAAGAGATCAAAAGAAGCTTTCCCGTT CTATCAAAAGGCTTTGGAATATACCGAGATCACCAAAGATGAAAAAAGTCTTGAGTGTGTACAAGTCCTG AGGGAACTGGCAGGTGTGGAGCAAGCCCTAGGACTCTACGCCGCCGCCATCAGTCATTTCTCACGGGACC GACTGCCAACTCCCCAGCCTTGTCCCCTGGGACACAAGTGCTGCTGCCCCTCTCCGTTCTTGTCACCAGT CCTTAATGTCACATGGAGACCCTTCTACAGCAGTCAGGTGGATGAGGAAGAGGCACATCTCATCATCCTA AGTAAAGATCCCTCCCCGGAGGAGGCTGCAGACTCTGCACACTTCATCGCCCGGGCTGCTGCTGCTTCTG GGATGCACGACCACCGTGATGTGGCCGAGAAATACTTTCAGGAAAGCATGACTTCTATCAAGGATTCCGA AGGAGCGGAAAGAGCCAAGTTCCTTTCGATTCAAGATGAGTTTTGCAGTTTTCTGCAAACCACCGGACAA AAAGAAAGAGCGGCCATGATCCTGAGAGAGTCCCTGGAAGCCAAAGTAGGAGCATTTGGTGACTTCAGTC CTGAGGTGGCAGAAACGTACCGGGCCCTGGGCAGGGCAGACCTGGCGCAGGGAAACAACAGTGGAGCGTA CGCGAAGCTAAAGAAGTGTGTCCAGATTGAGACGTTCTTGTATGGATCACAGGACAAGAAGACCCTGGCC ACCCAGCACACCATAGACACACTGTCAAAGATCTCCGAGGCTGCCGGGAAGTCAAGGCAATCGGTGAAAG CCAAAGTAGCATTCTGCACCAGTGCCCCCCAGTACGGCATGCCAGGGAAGGGCAGGCACAGTGTAGCAGA CTGATGCGCCCATGTGCCGCCCCAAAGAAAGCTCCAAGAAAGACTTGGCATTGCTTGTAGGATGCTGAAC AAACCTCTCTCCAGCTCGAAGGTGAAGTTCAGCAGCCACTGCAGGTTTTTAAGGCTGTTTACTTCAGAAT GCAATGGCAGTACCATGAATCTTCCACACCCCAATTGTGAATGCCCTTTGGGGTGATATGGTGCTCTTGG TTATCTTCTTTCACCAAGGAGACTCTCCTCTGTCATCTCTGCCGTGGCTGAAACACTCTTCCCAAGTTGG AGCTTCTTGACAGGAAAGGAGACTCACCTGGTGACAGGCAGGGCTCCTGCTCCATCCAGAGCCACATCTG GGGCAGCGAGACCCTGCACAAAGCCTCCCTCATATGAAAGTAATAGTAACTGAGAAGAAACTACCAATAA AGAGATGCTTGTGCTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4485073 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4485073 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14227 same embryo
	EMAGE:14226 same embryo
	EMAGE:14224 same embryo
	EurExpress:euxassay_003967 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source