EMAGE mouse gene expression spatial database

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EMAGE:14234

Dapp1 dual adaptor for phosphotyrosine and 3-phosphoinositides 1 ( MGI:1347063)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003992. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003992_01	euxassay_003992_02	euxassay_003992_03	euxassay_003992_04

euxassay_003992_05	euxassay_003992_06	euxassay_003992_07	euxassay_003992_08	euxassay_003992_09

euxassay_003992_10	euxassay_003992_11	euxassay_003992_12	euxassay_003992_13	euxassay_003992_14

euxassay_003992_15	euxassay_003992_16	euxassay_003992_17	euxassay_003992_18	euxassay_003992_19

euxassay_003992_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14234_wholemount_strong.wlz

14234_wholemount_moderate.wlz

14234_wholemount_weak.wlz

14234_wholemount_possible.wlz

14234_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14234_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 08 09 11 12

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 13 moderate expression: see section 08 10 12 14 15

liver left lobe

strong

single cell

strong expression: see section 01 02 03 04 05 moderate expression: see section 06 07 08 09

liver right lobe

moderate

single cell

moderate expression: see section 10 11 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4178
Entity Detected:	Dapp1, dual adaptor for phosphotyrosine and 3-phosphoinositides 1 ( MGI:1347063)
Sequence:	sense strand is shown >T4178 CAGAGACTCAGCCCCAGCCGGCTCCTGCCTTAGCGAGGAGGAGCAGCGTGTCCAGCAGCAGCCGATCCCA TCTCTCTCTTCATACAGCACCTGGCGGGGAGTCAGCCATCAGAAGCAAAGGAACTTATGGGCAGAGCAGA ACTTCTAGGAGGGAACATGAGTACCCAGGATCCCTCAGAGCTGTGGGGCAGAGCAGATGGAGGCACAGAC CTGCTCCAGGATTTGGGGTGGTATCACGGCAACCTCACCCGCCATGCTGCCGAAGCCCTTCTGCTCTCCA ATGGACGTGATGGTAGCTACCTGCTGCGGGACAGCAATGAGCAGACTGGGCTCTACTCTCTCTCCGTGAG AGCCAAAGACTCTGTCAAACACTTTCATGTTGAATACACTGGGTACTCGTTTAAATTCGGCTTTAATGAG TATTCATCTCTAAAGGATTTTGTCAAGCATTTTGCAAATCAGCCTTTGATTGGAAGTGAGACAGGGACTC TGATGGTTTTGAAGCATCCCTATCCAAGGGAAGTGGAAGAACCTTGCATTTACGAATCAGTCCGGGTTCA CACAGCGATGCAGACAGGAAGGACAGAGAACGACCTCGTGCCCACTGCACCTTCTCTGGGCACCAAAGAA GGCTATCTCACCAAGCAGGGAGGCCTGGTAAAGACCTGGAAAACAAGATGGTTTACTTTGCAAAGGAACG AACTGAAATATTTCAAAGACCAGATGTCACCAGAACCAATTCGGATCCTAGACCTAACAGAGTGCTCAGC TGTTCAATTTGATTACTCACAGGAACGAGTAAACTGTTTCTGCCTAGTGTTTCCATTCAGGACATTTTAT CTCTGTGCAAAGACGGGAGTTGAAGCCGATGAATGGATCAAAATACTGAGATGGAAGTTGTCAAAAATAA GAAAACAGCTGGATCAAGGAGAAGATACCGTCCGATCTAGGTCATTTATCTTCAAATAGACTTGTGTGCT CAGGGGCACGGTTGACTGTTCCAGGTGTCTGATCTGTGGCAGACTTCAGAGGAAAACTCATTGAAGTATT TCCTCTAAAAACAAATCAAAAGTAGATCGTGGTTGGGAGCTGTCTGCTGTCTGCTGATCCATGGAAAACA GCTGGCAGGACCTGGCCATCTGCTCGAGAACAGAAGCAGCGCCCTGCTGATGGCAACTGCCTAGCTTCCA GGTGGAGCCACTCTCAGACACAGCGCCAGACAAGAGCTCATTGGCTGTCCCAGTGGCTCAGGCTCATTGA CTCTAGTTTCCTGGAGTTCAGCCCCAAGTCATGGTCAGCACAACTAAACTTCAGGACTTGCTACAAGCAC AAGGAAACAGCAGTGCATCAGGGAGCTCGGGCTGGGCTTTAGGAAGCAGATTAATAAACACCAAGGACAT TCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4486834 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4486834 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14235 same embryo
	EurExpress:euxassay_003992 same experiment
	MGI:4824233 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source