Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14253

Abi3 ABI gene family, member 3 ( MGI:1913860)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253
"Pseudo-wholemount" of euxassay_004063. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004063_01 euxassay_004063_02 euxassay_004063_03 euxassay_004063_04
EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253
euxassay_004063_05 euxassay_004063_06 euxassay_004063_07 euxassay_004063_08 euxassay_004063_09
EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253
euxassay_004063_10 euxassay_004063_11 euxassay_004063_12 euxassay_004063_13 euxassay_004063_14
EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253 EMAGE:14253
euxassay_004063_15 euxassay_004063_16 euxassay_004063_17 euxassay_004063_18 euxassay_004063_19
EMAGE:14253 EMAGE:14253 EMAGE:14253
euxassay_004063_20 euxassay_004063_21 euxassay_004063_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14253Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14253_wholemount_strong.wlz
14253_wholemount_moderate.wlz
14253_wholemount_weak.wlz
14253_wholemount_possible.wlz
14253_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14253_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 09 weak expression: see section 08 10 11 12
heart ventricle
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5558
Entity Detected:Abi3, ABI gene family, member 3 ( MGI:1913860)
Sequence:sense strand is shown

>T5558
GCCATGGCGGAGCTGCAGCAGTTACAGCAGCTGCAGGAGTTTGATATCCCCACGGGCCGGGAGGCTCTGC
GGGGCAACCACAGCGCCCTGCTACGGGTGGCCAACTACTGCGAGGATAACTACTTGCAGGCCACAGACAA
GCGGAAGGCGCTGGAAGAGACGATGGCTTTCACCACCCAGGCCCTAGCCAGTGTGGCCTACCAAGTGGGT
AACCTGGCCGGGCATACTCTTCGAATGCTGGATCTACAGGGTGCTGCCCTGCGGCAGGTAGAAGCCAAGA
TGAGCACACTGGGCCAGATGGTAAACATGCATATGGAGAAGGTAGCTAGAAGGGAGATCGGCACTTTGGC
CACTGTTGTGCGGCTGCCCTCTAACCAGAAAGTCATCCCTCCTGAGAGCCTGCCTTCCCTCACTCCCTAC
CACAGAAAACCCCTCAACTTTGCCTGCTTGGATGACATTGGCCACGGAGTCAAGGACTTGAGCACGCAGC
TGTCACGGACCGGGACCCTGTCTCGCAAGAGCATAAAGGCGCCTGCTACACCTGTTTCCGCCACGCTGGG
GAGACCACCCCGGATCCCTGAGCCGGTGCAGCTCCCAGCCGTGCCAGACGGCAAGCTCTCCGCTGCCTCC
TCTGCCTCTTCCTTGGCCTCGGCAGGCAGTGCGGAAGGTGCCAGTGGGATCCCCCAGTCCAAGGGACAGG
TAGCGCCTGCAACCCCGCCTCCTCCACCTGTAGCACCTGTAACCCCGCCTCCTCCACCGTTGTCTGCCGA
GGTCTTCTTGCCGCCCCCTCCGCTGGAGGTGTCCCAGCCCCCTCTGGAAGCAGAGTTGCCCCTGCCTCCT
CCCCCAGCTCTAGAGGGGGATGAACTGGGGCTGCTGCCTCCGCCCCCACCAGGTTTTGGACCAGATGAGC
CCAGCTGGGTCCCTGCTAGCTACTTGGAGAAAGTGGTGACCCTGTACCCATACACCCGGCAAAAGGACAA
TGAGCTCTCCTTCTCTGAAGGCACCGTCATCTGTGTCATTCGCCGCTACTCGGATGGCTGGTGTGAGGGT
GTCAGTTCAGAGGGCACTGGATTCTTCCCAGGGAACTATGTGGAGCCCAGCTGCTGACAGCCCAGGATCT
ATCCCTGCACCAACCCTGCACTGCCTCGGTGGGCCTCTCGAGCCCCAGGAAGCCAGCTTCCACAGTCAAA
GCTGGACTAAGGACCTGTCTACCTCTTGGGCTGTGAACTGTGTTCAGTCCCTGCTCCATACAGCAGTGGG
AAGAGGGTGGGCTAGAGAGGGGTGGTACTCAGGGGGGACTGCTGCAGATGGCATAAAGAATACAAAAACA
AACAAACCTAGAAGGCAAGGAGTTTAAGCACCTTGCCCAGAGGACCCCCAGCTCATGCTCAGAAGATCGC
CAGCTTTGAATAAAACTGCTGACCTCCAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6474610 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6474610 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14250 same embryo
 EMAGE:14249 same embryo
 EMAGE:14252 same embryo
 EMAGE:14248 same embryo
 EMAGE:14251 same embryo
 EurExpress:euxassay_004063 same experiment
 MGI:4822882 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS