EMAGE mouse gene expression spatial database

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EMAGE:14273

Gne glucosamine ( MGI:1354951)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004086. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004086_01	euxassay_004086_02	euxassay_004086_03	euxassay_004086_04

euxassay_004086_05	euxassay_004086_06	euxassay_004086_07	euxassay_004086_08	euxassay_004086_09

euxassay_004086_10	euxassay_004086_11	euxassay_004086_12	euxassay_004086_13	euxassay_004086_14

euxassay_004086_15	euxassay_004086_16	euxassay_004086_17	euxassay_004086_18	euxassay_004086_19

euxassay_004086_20	euxassay_004086_21	euxassay_004086_22	euxassay_004086_23	euxassay_004086_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14273_wholemount_strong.wlz

14273_wholemount_moderate.wlz

14273_wholemount_weak.wlz

14273_wholemount_possible.wlz

14273_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14273_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 06 07 08 18 19 weak expression: see section 16 17

rectum

strong

regional

strong expression: see section 12

rectum lumen

strong

regional

strong expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5575
Entity Detected:	Gne, glucosamine ( MGI:1354951)
Sequence:	sense strand is shown >T5575 GCTAAACCAGAGGCCAGACGGCAGCTCAGGAGTCCGACCACACCTCAGGAAACAGCTGTGCCACAGGATG GAAACACACGCGCATCTCCACAGGGAGCAGAGCTACGCAGGACCTCATGAACTCTATTTTAAGAAACTCT CAAGTAAAAAGAAGCAAGTCATGGAGAAGAACGGGAACAACCGAAAGCTCCGGGTTTGCGTTGCCACCTG CAACCGAGCTGACTACTCCAAACTGGCCCCGATCATGTTCGGCATCAAGACAGAGCCCGCGTTCTTTGAG TTGGACGTGGTGGTGCTCGGCTCCCACCTGATTGACGACTATGGAAACACATACCGCATGATTGAGCAAG ATGACTTTGACATTAACACCAGGCTCCACACGATTGTTAGAGGGGAAGATGAAGCGGCCATGGTAGAGTC GGTAGGCCTAGCGCTCGTGAAGCTACCGGACGTCCTCAATCGCCTGAAGCCCGACATCATGATTGTTCAC GGAGACCGATTTGACGCCCTTGCTCTGGCTACGTCTGCTGCCTTGATGAACATCCGCATCCTTCACATTG AAGGAGGCGAGGTCAGCGGGACCATTGATGACTCTATCAGACACGCCATAACAAAACTGGCTCACTACCA TGTGTGCTGCACTAGAAGTGCAGAGCAGCACCTGATCTCTATGTGCGAGGACCACGACCGCATCCTGTTG GCAGGCTGCCCTTCCTATGACAAACTGCTCTCCGCCAAGAACAAAGACTATATGAGCATCATTCGGATGT GGCTAGGCGATGATGTAAAATGTAAGGATTACATCGTTGCCCTGCAGCATCCCGTGACCACTGACATTAA GCATTCCATAAAGATGTTTGAGCTAACACTGGATGCCCTGATCTCGTTTAACAAGAGGACCCTAGTTCTG TTTCCAAATATCGATGCAGGCAGCAAGGAGATGGTTCGAGTGATGCGGAAGAAGGGCATCGAGCATCACC CCAATTTCCGTGCAGTCAAGCACGTCCCGTTTGACCAGTTCATACAGCTGGTCGCCCACGCTGGCTGCAT GATTGGGAATAGCAGCTGCGGCGTGCGAGAGGTTGGCGCTTTCGGAACACCCGTGATCAACCTGGGCACA AGGCAGATAGGAAGAGAAACCGGGGAGAATGTTCTTCATGTCAGGGATGCTGACACCCAAGATAAAATAT TGCAAGCACTACACCTCCAGTTCGGCAAACAGTACCCTTGCTCAAAGATATATGGGGATGGGAATGCTGT TCCAAGGATTTTAAAGTTTCTCAAATCCATTGACCTTCAAGAGCCACTACAGAAGAAATTCTGCTTCCCC CCTGTAAAGGAGAACATCTCTCAAGACATTGACCACATCCTGGAAACTCTGAGTGCCTTGGCTGTTGATC TTGGCGGGACAAACCTGAGGGTGGCAATAGTTAGCATGAAGGGTGAAATCGTTAAGAAGTACACTCAGTT CAACCCTAAAACCTATGAAGAAAGGATTAGTTTAATCCTGCAGATGTGTGTGGAAGCTGCCGCGGAAGCT GTGAAACTCAATTGCAGAATTCTGGGAGTAGGCATCTCCACAGGTGGCCGCGTGAATCCCCAGGAAGGAG TTGTGCTGCATTCAACCAAGCTGATCCAGGAATGGAACTCCGTGGACCTCAGGACACCCCTCTCCGACAC CCTGCATCTCCCCGTGTGGGTGGACAATGACGGCAACTGTGCCGCCATGGCAGAGAGGAAGTTCGGCCAA GGAAAAGGACAGGAGAACTTCGTGACGCTCATCACGGGGACAGGGATCGGTGGGGGGATCATCCACCAGC ACGAACTGATCCACGGCAGCTCCTTCTGCGCGGCGGAGCTCGGCCATCTCGTGGTGTCCCTGGACGGTCC TGACTGCTCCTGTGGAAGCCATGGGTGCATCGAAGCGTACGCCTCTGGAATGGCCTTGCAGAGGGAAGCA AAGAAACTCCATGATGAGGACCTGCTCTTGGTGGAAGGGATGTCAGTACCAAAAGACGAAGCTGTGGGTG CCCTCCATCTCATCCAGGCTGCCAAGCTGGGCAACGTGAAGGCCCAGAGCATCTTACGAACAGCTGGAAC TGCTTTGGGACTTGGGGTTGTGAACATCCTCCACACTATGAATCCTTCCCTGGTGATCCTGTCTGGAGTC CTGGCCAGTCACTACATCCACATCGTGAAGGACGTCATCCGCCAGCAAGCCTTGTCCTCCGTGCAGGATG TGGACGTGGTGGTCTCAGACTTGGTGGACCCGGCCCTGCTTGGCGCAGCCAGCATGGTTCTGGACTACAC AACGCGCAGGATCCACTAGGTCTCCCGGGAACGGACACGGACAGAGACTCGGGAGCTGCTTAGAGTGGAA CCATGCTCTTCTAGATCAGTGTTTCTGCGAAGGCAAATTTGGGGGGAGGGCTGCTGAGACAGCTCAGTGG TTAAGAGCCTGCCCTGCTCCTGCCAGTCCCCAGCACCCATGTCAGGCAGCTCAGCTGCCTGGAAGCCAAG CTCCAGGGGACCCAATGCCTCTCTGCCGGGGGCAGCTGCACTCAGATGTACATACCCCTCTCCACACACA TACAAATAAAGCTTATTTTTCAAAAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6478532 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6478532 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14277 same embryo
	EMAGE:14274 same embryo
	EMAGE:14275 same embryo
	EMAGE:14276 same embryo
	EurExpress:euxassay_004086 same experiment
	MGI:4825134 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source