EMAGE mouse gene expression spatial database

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EMAGE:14275

Krt14 keratin 14 ( MGI:96688)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004087. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004087_01	euxassay_004087_02	euxassay_004087_03	euxassay_004087_04

euxassay_004087_05	euxassay_004087_06	euxassay_004087_07	euxassay_004087_08	euxassay_004087_09

euxassay_004087_10	euxassay_004087_11	euxassay_004087_12	euxassay_004087_13	euxassay_004087_14

euxassay_004087_15	euxassay_004087_16	euxassay_004087_17	euxassay_004087_18	euxassay_004087_19

euxassay_004087_20	euxassay_004087_21	euxassay_004087_22	euxassay_004087_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14275_wholemount_strong.wlz

14275_wholemount_moderate.wlz

14275_wholemount_weak.wlz

14275_wholemount_possible.wlz

14275_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14275_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 06 07 15 16 17 18

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 weak expression: see section 21

vibrissa

strong

regional

strong expression: see section 01 02 03 04 05 06 16 17 18 19 20 weak expression: see section 21

naris

strong

regional

strong expression: see section 08 09 11 12 13

nasal cavity respiratory epithelium

strong

regional

strong expression: see section 08 09 12 13

lower jaw incisor

strong

regional

strong expression: see section 08 09 10 12 13

lower jaw molar

strong

regional

strong expression: see section 06 16

oral epithelium

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

upper jaw incisor

strong

regional

strong expression: see section 08 09 10 12 13

upper jaw molar

strong

regional

strong expression: see section 06 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6174
Entity Detected:	Krt14, keratin 14 ( MGI:96688)
Sequence:	sense strand is shown >T6174 GCTCTCTCTTCTCTCTGCCGACTCACTCGCTCACTTGCTCACCTCTCTCAAAGCCATGGCCACCTGCAGC CGCCAGTTCACCTCCTCCAGCTCCATGAAGGGCTCTTGTGGTATCGGTGGTGGCTCTAGCCGCATGTCCT CCATCCTGGCTGGAGGATCCTGCCGGGCTCCCAGCACCTACGGGGGCATGTCGGTTACCTCCTCTCGCTT CTCCTCTGGGGGAGCCTGTGGGATTGGAGGTGGCTATGGTGGGAGCTTCAGCAGCAGCAGTTTTGGTGGA GGACTTGGTAGTGGATTTGGTGGTCGATTTGATGGATTTGGTGGTGGTTTTGGTGGTGGTCTTGGTGGTG GTTTTGGTGGTGGTCTTGGTGGTGGTCTTGGCGGTGGTATTGGTGATGGGCTCCTGGTGGGCAGTGAGAA AGTGACCATGCAGAACCTCAATGACCGCCTGGCCACCTACCTGGACAAGGTGCGTGCCCTGGAAGAGGCC AACACTGAACTGGAGGTGAAGATTCGGGACTGGTACCAGAGGCAGCGGCCCACTGAGATCAAAGACTACA GCCCCTACTTCAAGACCATCGAGGACCTGAAGAGCAAGATCCTGGCAGCCACCGTGGACAATGCCAATGT CCTCCTGCAGATCGACAATGCCCGCCTGGCTGCCGATGACTTCCGGACCAAGTTTGAGACGGAGCAGAGC CTGCGCATGAGCGTGGAGGCCGACATCAATGGCCTGCGCAGGGTGCTGGATGAGCTGACCCTGGCCAGAG CCGACCTGGAGATGCAGATTGAGAGCCTCAAGGAGGAGCTGGCCTACCTGAAGAAGAACCACGAGGAGGA AATGGCCTCCATGAGAGGCCAGGTGGGTGGAGACGTCAATGTGGAGATGGACGCCGCCCCTGGTGTGGAC CTGAGCCGCATCCTGAACGAGATGCGGGATCAGTACGAGAAGATGGCGGAGAAGAACCGCAAGGATGCTG AGGAATGGTTCTTCAGCAAGACAGAGGAGCTGAACCGCGAGGTGGCCACCAACAGCGAGCTGGTGCAGAG CGGCAAGAGTGAGATTTCTGAGCTCCGGCGCACCATGCAGAACCTGGAGATCGAGCTGCAGTCCCAGCTC AGCATGAAAGCATCCCTGGAGAATAACCTGGAGGAGACCAAAGGCCGTTACTGCATGCAGCTGGCCCAGA TCCAGGAGATGATCGGCAGTGTGGAGGAGCAGCTGGCTCAGCTGCGCTGCGAGATGGAGCAGCAGAACCA GGAGTACAAAATCCTGCTGGATGTGAAGACAAGGCTGGAGCAGGAGATCGCCACCTACCGCCGTCTGCTG GAGGGAGAGGACGCCCACCTTTCATCTTCCCAATTCTCCTCATCCTCTCAATTCTCCTCTGGCTCTCAGT CATCCAGAGATGTGACCTCCACCAACCGCCAGATCCGCACCAAGGTCATGGATGTGCACGATGGCAAGGT GGTCTCCACCCACGAGCAGGTCCTGCGCACCAAGAACTAAAGCTGCTACATGCTGCTCAGGCTTAGGAGG CTCCTGCGTGGACGCAGATACTGCTGGAAGAGCCCCTGTATTGTCCCTATAGGCTTCACTCTTTACTTGA CCCTTGTCCTTCCTGGCAAGCAATAAAGCTTCTTTTTCTGAGTTGCACAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3497492 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3497492 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14277 same embryo
	EMAGE:14274 same embryo
	EMAGE:14273 same embryo
	EMAGE:14276 same embryo
	EurExpress:euxassay_004087 same experiment
	MGI:4825827 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source