EMAGE mouse gene expression spatial database

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EMAGE:14277

Agpat4 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) ( MGI:1915512)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004078. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004078_01	euxassay_004078_02	euxassay_004078_03	euxassay_004078_04

euxassay_004078_05	euxassay_004078_06	euxassay_004078_07	euxassay_004078_08	euxassay_004078_09

euxassay_004078_10	euxassay_004078_11	euxassay_004078_12	euxassay_004078_13	euxassay_004078_14

euxassay_004078_15	euxassay_004078_16	euxassay_004078_17	euxassay_004078_18	euxassay_004078_19

euxassay_004078_20	euxassay_004078_21	euxassay_004078_22	euxassay_004078_23	euxassay_004078_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14277_wholemount_strong.wlz

14277_wholemount_moderate.wlz

14277_wholemount_weak.wlz

14277_wholemount_possible.wlz

14277_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14277_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5572
Entity Detected:	Agpat4, 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) ( MGI:1915512)
Sequence:	sense strand is shown >T5572 CTCAGGCTCCTGCCTCGCAGTTCTGGCTTGTGAGCACCAATGCAAAGAACTGCCCGGAGCGAGACACTTT CCTGAGCCCCGGATCTTTCCCCCTTCTGGAACTATCCCACCATACGGATTTATCTCTTGAGAATCCCCAC ACCATGGACCTCATCGGGCTGCTGAAGTCCCAGTTTCTATGTCACCTGGTCTTCTGCTACGTGTTCATCG CCTCGGGGCTCATTGTCAACGCCATCCAGCTGTGCACGCTGGTCATCTGGCCCATCAACAAGCAGCTGTT CCGCAAGATCAATGCCAGACTCTGCTACTGCGTCTCCAGCCAGTTGGTGATGCTTCTGGAGTGGTGGTCG GGCACGGAGTGTACCATCTACACCGACCCGAAGGCCTGCCCCCACTACGGGAAGGAAAACGCCATCGTGG TCCTCAATCACAAGTTTGAGATTGACTTTCTCTGTGGCTGGAGCCTGGCTGAGCGCCTGGGGATCCTGGG GAACTCCAAAGTCCTGGCCAAGAAAGAACTGGCTTATGTCCCAATCATTGGCTGGATGTGGTACTTCGTG GAAATGATCTTTTGCACACGCAAGTGGGAGCAAGATCGGCAGACGGTTGCCAAGAGCCTGCTGCACCTCC GGGACTACCCAGAGAAGTATCTGTTCCTGATCCACTGTGAGGGCACACGGTTCACAGAGAAGAAACACCA AATCAGCATGCAGGTGGCCCAAGCCAAGGGGCTGCCCAGCCTCAAACACCACCTGCTGCCGCGCACCAAA GGCTTTGCTATTACTGTGAAGTGCTTGCGAGATGTTGTCCCAGCTGTATATGACTGTACACTCAATTTCA GAAACAATGAAAACCCAACACTGCTGGGAGTCTTAAATGGAAAGAAATATCACGCTGACTGCTACGTTCG GAGGATCCCCATGGAGGACATTCCGGAGGATGAGGACAAGTGCTCTGCCTGGTTACACAAGCTCTACCAG GAGAAGGATGCCTTTCAGGAGGAATACTACAGGACAGGGGTCTTCCCAGAGACTCCCTGGGTTCCCCCAC GGCGGCCCTGGTCTCTGGTCAACTGGTTGTTCTGGGCATCGCTGCTGCTCTACCCTTTCTTCCAGTTCCT AGTTAGCATGGTCAGCAGCGGTTCCTCGGTGACGCTGGCCAGCTTGGTCCTCATCTTCTGTATGGCCTCC ATGGGAGTTCGATGGATGATTGGCGTGACAGAAATCGACAAGGGCTCTGCCTACGGCAACATCGACAACA AACGGAAACAAACGGACTGATGTGGGAAAGTCACCTCCAGAGGGAACCGTGGGGGACGGCTGGCCTCTGC TTAGCCTTTGTAGCAGGGCTCAGTGATGGAGACTGGGGGGCCCTTGCTGGGACAAACACAAACCACAGCC CCTCTGGTCACGGAGTTTTGCCTCAGCGCTGGATGGGAAAGGAAGACGGGTTTAGACCTTTCCCCTCCCC TCCTCCCTGTGTGATATGGTTTCGGTTATGTCTCTTTTCAGTTGCGCGTGCGTGCGTGCGTGCGTGTGTG TGTGTGTGTGTGTGTGTGTGTGTGTGTGAGTGTGTGAGAGAGAGAGGGAGAGAGAGAGAGAGAGAGATGG GGTGAGGAGAATGGGTGTGTGGTTGCGTGTGCACTTGTGACCCTGTAACAGTAGGGTCTGGGAGGCTGCA AGGAAGGGCAGGGCCAGGGTGGAAGGGAAGGTGTCCCTGTCACCCCATGGTGCTGCGTCTTCTCTAACCC TCGATTGCCGGAGACAGAGTGAAAAGTGCTTTGGGTAAGATGACTAAATTATGCCTCCAAATAAGAAAAA GAATTAAAGTGTTTCTCTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6477257 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6477257 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14274 same embryo
	EMAGE:14273 same embryo
	EMAGE:14275 same embryo
	EMAGE:14276 same embryo
	EurExpress:euxassay_004078 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source