EMAGE mouse gene expression spatial database

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EMAGE:14288

Ap1s2 adaptor-related protein complex 1, sigma 2 subunit ( MGI:1889383)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004097. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004097_01	euxassay_004097_02	euxassay_004097_03	euxassay_004097_04

euxassay_004097_05	euxassay_004097_06	euxassay_004097_07	euxassay_004097_08	euxassay_004097_09

euxassay_004097_10	euxassay_004097_11	euxassay_004097_12	euxassay_004097_13	euxassay_004097_14

euxassay_004097_15	euxassay_004097_16	euxassay_004097_17	euxassay_004097_18	euxassay_004097_19

euxassay_004097_20	euxassay_004097_21	euxassay_004097_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14288_wholemount_strong.wlz

14288_wholemount_moderate.wlz

14288_wholemount_weak.wlz

14288_wholemount_possible.wlz

14288_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14288_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

urinary system

strong

regional

strong expression: see section 04 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5581
Entity Detected:	Ap1s2, adaptor-related protein complex 1, sigma 2 subunit ( MGI:1889383)
Sequence:	sense strand is shown >T5581 GGCACCACGGCTTCCCTTCCACAGCACGGCGACGGGGGCTTCCCCTCAGCAGCTGCCGGCGCCTCAGTCT CCGCGGCCAGCCCAGCTCCGCCGCGTCCGCGGCCCGCCGCCGCCATGCAGTTTATGTTGCTTTTTAGTCG CCAGGGAAAGCTTCGACTGCAGAAATGGTATGTCCCACTGTCAGACAAAGAAAAGAAGAAGATCACAAGA GAACTTGTTCAAACCGTTTTAGCACGGAAACCCAAGATGTGCAGCTTCCTTGAGTGGAGAGATCTGAAGA TTGTTTATAAAAGATATGCCAGTCTATATTTTTGCTGTGCTATTGAGGATCAGGACAATGAACTGATTAC CCTGGAAATAATCCATCGTTACGTGGAATTACTTGACAAGTATTTTGGCAGTGTATGTGAACTTGATATC ATCTTTAATTTTGAGAAGGCCTATTTTATTTTGGATGAATTTCTCTTGGGAGGAGAAGTTCAGGAAACAT CCAAGAAAAATGTCCTTAAAGCAATTGAGCAGGCCGATCTCCTGCAGGAGAAAACAGAGACTATGTATCA CAGCAAGAGTTTCATTGGGTATAAGAAAGCGTACTAGAATACGTGCTGGAAGTACAGACATGCATCAAAA TGTAGTAGGATATATGAATTCAGTTCTAATTATCATTTTGTCAAGTTTATTATTTGTGTTTTGGTATTAT TTGGAAAATTAATTGCAATACCAATGGATAGCCCTATTATTTCATTAAATTGATGGGTTTTAGGAAATTT TCCCTGTAATTCATAATTGAGATTATAATTTACCTTTTATACAGAATATTGAAATTGGGTGAGACTCATT GACAAAGTATTCTTAAATATTGTAAATCAGTAATGTTATCACAGATGAAGCCTTCCCTGTGAGAACGAAA GCTTCTATAAAGTGAAGCACTGTTACTAATGTTGCTTTACTTGTTTTATCCTTGCTCTATTCTAAATGGG AAAGGGGCAGCTTTTGTAGCCTAATATTTTAGAGTTCTAACTTTAAAATTCATCGGGGATGCTGTAAGGT CCATTTCAGACCCCACCTTGGGATATTTTTGTCACTTTTTGATGTGAAAACATCTAACATCTTGTCAGAT ATATTTATAAGCAGATGTTTAAAAATTTTAGGGAGTTCCTTAGAATCATTTTGAGGACTTTCAAAAAAAT TAATCTTTTAGCTTCAAAGTCACATTTTTTTAAAGTAAGTCAACAATTAAAAGCTTTCATACTTAACAAT ACCATATGATGGCTAGTTTTAACTTGTTGGGAGGAAGCCTTTTCTCTGGACCTCTCCTTAGTGTAATAGG ACCCTGATGAATGTGACACACTGGTGAAAGGGAGTTTTCTAACTGAACTTGCCACTAATTAGCATTTACT TAAACTGGCAATCTTCTCAGATTAGTAGTAAGTACAGTGCTTTAGGATTCTGTGAAATGTAGGTTATCTT GTTTACTGTTACATACCATGAACTTGAAAAAGATAAATCTGAACTGGGCATTAAGAACTTAAGTGTTCAG CCACAAACCAAAGAACAAAAGCAAATCTGATGCTTTAGATCATTTTACTGTGAACTAAACATTCTGATTG TGTCTCCATCAGCTTGTGTACTGTGTGTAAGAATATATGCATTTCTTTTGTAGCCACGCCATGAATATTT TAATGTCCCTGTGTACTAATGACTGCTCATCGACTATGCAGACTTTCACAGAGGAAATAGAGAACTGATA TACTTATGAAAGAAAATGTCCTCAGCTTGGTGACTAATTCTCCTGTAAAATAATATATAGCATTTTATAG CTAGTACCTTTATGATTGTATTTTTCTGTGCCTATTCCTAATTCTATTTATTCTGTATATGATATGACAT AGTTTTTTCTGTCTACTACTGATTTCTTCTTTACAAAATATGACTTGTGAATGTTTTAATATTCTAAACA CATACAAGTTATCATTAGTAAATCATCTGTGATTAATCTTATGAGGGTATAAGAGAAAGACCGGGAACAA TCACAAATGTTTACCAAAGAAAATAGTTAGGAACTCAGGCAAAATTAGGAGCACCACAATATTTAATGTA GTTTGATCTTCCACTGTTGAAGAAAACCAAGCTTTATTTAGCACTAAGATTACTTTATAGTCTGTACAAG CTAATATGTTGGACAGTATCACATAAAAATGTGTTGAAGCAATATAAGAAAAAAATCAATTGTATAATTC TCTAAGATCTAAGGTTAAAACATTTCAATTTTTAAGAAATTTTGTATTGCCAATATTTATTAAGCCACAT TTATGCAGTGTCAAAAGTCCTTTAAGAACTATCAGAAAGCTATGTCACAAAGTTTCTTGTATTGTTGCTC AGTAACAGCCCTTGAGTCAGTAGTACTGAGCCTACTTATGTGATACAAAATTCTGATATTCATTTTCAAT ATTCAAACATTTATACTTATTAGTTAATGGCTTCCTAATTAATATATTAAGCCTTCTTCTAAACACTCAG TGCTGTGTTAACAATGGTTGTATATTTTGGAATACTAAAAGTTTTTAATATTAATTTTATAAAAATATGC TTTTAATAATATTTATCAAATTCTACTATTAAAATACCAGATAACTCATTTGTCTATCTAGAGAGTTTAA AACCTAAGTAAATTGTTACTAAACAGTAACTTAAGTGGCATGGTAGTGCATGCCTGTCATCCTAGTACCT GGGAGGCTGATGCAGGAGCAAGATGAGTTACTATAGTACCTAGGGCATCCCTGTCTCAAAAGACAAAAAC TAAATATGCCAAAAACTCTAGGAACTTCAGAAGGTACCCAAATGAGTTTCCCACTGAAAGAAGATCTATA AAATGTCTTAGGAAATGTAGTGTTAGGCAGCTGTACAGGTCAGCTCTCTCCATTACCATCTGAAACTTCA CAAAACATTGGTCTTTCATTTCCATTTTTCCCATCCTCCCACACTACATGTTGTTCACCTGATTTTAATG TTGCATAAATATGCATTGTCTCATGCCTGTTTGTTTTGGATGCTGTGTGCTGAGCTGTAACAAAATGTTG TGTTTTTGTGAGGGGGAGGGGGTATCTGTATACAGCTGCTTGCTTTAAATTAGCACATTCTCAAATAGCT TTTCCTGCATGTCTGAAATAAAGTATTGCATGTAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6485320 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6485320 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14290 same embryo
	EMAGE:14287 same embryo
	EMAGE:14291 same embryo
	EMAGE:14289 same embryo
	EMAGE:14292 same embryo
	EurExpress:euxassay_004097 same experiment
	MGI:4823160 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source