EMAGE mouse gene expression spatial database

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EMAGE:14290

Zfp747 zinc finger protein 747 ( MGI:2443581)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004077. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004077_01	euxassay_004077_02	euxassay_004077_03	euxassay_004077_04

euxassay_004077_05	euxassay_004077_06	euxassay_004077_07	euxassay_004077_08	euxassay_004077_09

euxassay_004077_10	euxassay_004077_11	euxassay_004077_12	euxassay_004077_13	euxassay_004077_14

euxassay_004077_15	euxassay_004077_16	euxassay_004077_17	euxassay_004077_18	euxassay_004077_19

euxassay_004077_20	euxassay_004077_21	euxassay_004077_22	euxassay_004077_23	euxassay_004077_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14290_wholemount_strong.wlz

14290_wholemount_moderate.wlz

14290_wholemount_weak.wlz

14290_wholemount_possible.wlz

14290_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14290_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5578
Entity Detected:	Zfp747, zinc finger protein 747 ( MGI:2443581)
Sequence:	sense strand is shown >T5578 CGGAAATCTCCGCTGTGCTCCTATCAGTTTTCACTTTTTCCCTACGTTAAGGTGCACACGGACCTGGTTC CTGTCCTTTTTCAGCCCTGGTACCCGAAAACGGACACGTTGGACTGGGCAGGCAATCCCAGGAGTGCGGG GCTGACCCAGGGACTGTGCGGGGAGTCTACATGGCGCAGGTGATGGCCCCGGGAAGGGCCTCGGTACCGC CTGTGTTCTGTAAACCTGGGACTGTGAGCTTCGCGGACGTGGCCGTGTACTTCTCCCCGGAGGAGTGGAG GTATCTGCGGCCGGCGCAGAGAGCCCTGTACCGAGAGGTGATGCGCGAGACCTACGGTCTCCTGACCTAT CTCGACGTCGGATGCGCCAAACCAGCGCTCATCAGCTGGGTGGAGGAAGAGTCCGAAGTGTGGGGACCCG GTGCCCAGGATCCAGAGGTGGCCATGTGCAGGACAGAAGTCCACTCAGATTGCAGGCACGAGAAGGAAAG GAAGAGACCAAGAGAAGAGACTGAAGCAATGCAGAAGACATTTTCTCCAGAACCTGGGCAGAAGGAGCCA CAACTCTCATTTGCTGTCTCCGCTAGTGGAATTCAAGCCACGGTCCTAAGGGCAGACAAGCGTCACGGGT GCCATTTGTGTGGGAAGAGTTTTGCCCGGCGGTCCACGTTGGAGGCACACATGTACACTCACACAGGTGA AAAGCCGTTTCAGTGCCCAGACTGCAACAAACGATTTGGCCGGTATTCTTCCCTGAGCACTCACCGAGCC ATACACCGAGGGGAACGGCCGCACCAATGTCCTGACTGTGGGAAGGGTTTTACCCAGCGGTGCACTTTGG TGGCACACATGTACTCTCACACAGGTGAAAAGCCGTTTCAGTGCCCAGACTGCAACAAATGCTTCGGCAG GTATTCCTCCCTGAGCATGCACCGAGCCATACACCGAGGGGAACGGCCACACCAGTGTCCTGACTGTGGG AAGGGTTTTACCCAGCGGTGCGCATTGGTGGAGCACATGTACTCTCACACAGGCGAAAAGTCATTCAATT GCCCTGATTGCTGCAAGTCCTTTGGTCGGCCATCTTCCCTGAGCAGGCACCGGGCCATACACCTCGAGGA GCGGCCTCATCGCTGCTCTCACTGTGGCAAGACTTTTACACAGCGCTCTTCACTCACCAGCCATCTCCGT GTCCACACTGGGGAGAAGCCTTACTGTTGTGCTGATTGTGGACGCTGCTTCAGCCAGCGCTCTAGGCTCC ATGAGCACCAGCGAGTGGTACACAGCGGTGTGACCCCCTTTTCTTGCACTCACTGTGGCAGTGCCTTTGC GGATTCCAAATATCTTCGGCGCCACATGCGCATCCATACAGGTGAGAAGCCTTATTCATGCCCTGACTGG TCGCTGCTTTCGCCAGGGCACCGAAATTCCAGCCCATAGGAGGACCCACACTGGCGAGCGTCCTTACCCC TGCCCTCAATGTGGCAGGCAGTTTCGCACAAAGTCAGCTATGACAAGCTACCAGTGGGTCCTTAGACCAG GATTAAAGGCCATAAGGACAAAAAAGCCAGTCAGTTATCTATATCCCTGGACCCTAGACAAGAGGACCCG GATCCACCTGTGGGCTTCTAGCATTACCCAGAGTTATTCCAAAAGTGCGGGTGATGGTCTTAAAGGCGCC AACATAGAGGGTGAAATATGTACATTGTTTGAGGCCCAGAGGATTGAACCTATGGCATCTTCCTGAAGTC ACAGACGTTCTAGGTACTGCTGGTCTGGGCACCGGGTACAGTCTTCTAGGAGGTTTCCAGATGCATCTGC ACACACTCCGGATGTACTGTCGTCTTGGCGTCTATTGGGCTGGGCTGGGCTGGGCAACCCCACTAGAGCT CCATGAGAAGCGAGGGCCTTTTCCTGGCTCAGAAGTATTGAGAAGCCTGAGGATGAAGAGAGCCTCGTTG AAGTCATTGTGTCTTTAATGCAGAGGCTAAAGTTGATGGCTCTGTGTGGGTTGAGCCAGCTGCATTCTCC GGGATTGAGTTACTGGTCAAGAGCCTGGTGTGGTGGTTCCTGCCTGTGATTCCCCCACTGGGGAGGTAGA GACAGGAAGACCAGGAATTTGAGACCAGTTTGGGTCACCTGAGACTGCATATAAACATAAATAAAATTAT TTATTTCTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6484930 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6484930 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14288 same embryo
	EMAGE:14287 same embryo
	EMAGE:14291 same embryo
	EMAGE:14289 same embryo
	EMAGE:14292 same embryo
	EurExpress:euxassay_004077 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source