EMAGE mouse gene expression spatial database

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EMAGE:14315

Sv2a synaptic vesicle glycoprotein 2 a ( MGI:1927139)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004125. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004125_01	euxassay_004125_02	euxassay_004125_03	euxassay_004125_04

euxassay_004125_05	euxassay_004125_06	euxassay_004125_07	euxassay_004125_08	euxassay_004125_09

euxassay_004125_10	euxassay_004125_11	euxassay_004125_12	euxassay_004125_13	euxassay_004125_14

euxassay_004125_15	euxassay_004125_16	euxassay_004125_17	euxassay_004125_18	euxassay_004125_19

euxassay_004125_20	euxassay_004125_21	euxassay_004125_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14315_wholemount_strong.wlz

14315_wholemount_moderate.wlz

14315_wholemount_weak.wlz

14315_wholemount_possible.wlz

14315_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14315_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5633
Entity Detected:	Sv2a, synaptic vesicle glycoprotein 2 a ( MGI:1927139)
Sequence:	sense strand is shown >T5633 CGCCGCTGCCGCCTCAGCCCTGTGGCTGGACCCCCTCCCTCACCCGGGGACTCCCTGACCCGGGGAACCA AGCTCAGGTCTTCAGAGCCTCTCAGAAGAAAAATAGGCAGCCCTCCCTGAAATATCTTGGATCCTCAGTT TAGCCTCTCCAACCTGGCTCCCCATTCCCAATCCCTCTCCCTCCCCCTCCCCCTCCCCCACTACTCACCC TACTGAACTGGGTGCAGAACAAAGCTCCTTTCTTCCTTTTTCCCCATCTGGACCTCACTGTCCAGGCCCT AGTTCCTCTCAGTCTGAGCCCAAAGATATTGGAACACAGTTCATCTGGAGAGAGGGTCTTCTTGACCCCT CCGATTCACTGAGCAAAGGGCTGAAAAAGAAGCAGAGAGTAAGGTAGATCCAGTGAAGTGCCCCAAGCCC CATCATGGAAGAAGGCTTTCGAGACCGAGCAGCGTTCATCCGTGGGGCCAAAGACATTGCCAAGGAAGTG AAGAAGCATGCGGCCAAGAAGGTGGTGAAGGGCCTTGACAGAGTCCAGGATGAGTATTCCCGAAGGTCCT ACTCCCGCTTTGAGGAGGAGGACGACGACGATGACTTCCCTGCCCCTGCGGACGGCTATTACCGCGGAGA AGGGGCCCAGGATGAGGAGGAAGGTGGCGCTTCTAGTGATGCCACCGAGGGCCACGATGAAGATGATGAG ATCTATGAGGGAGAATATCAGGGCATCCCCCGGGCAGAGTCTGGGGGCAAAGGCGAACGCATGGCAGATG GGGCACCCCTGGCTGGAGTGAGAGGGGGCTTGAGTGATGGGGAGGGTCCCCCTGGGGGTCGGGGGGAGGC GCAGCGGCGTAAAGATCGGGAAGAATTGGCTCAGCAGTATGAAACCATCCTCCGGGAGTGTGGCCATGGC CGCTTCCAGTGGACACTCTACTTCGTGCTGGGTCTGGCGCTGATGGCAGATGGTGTAGAGGTCTTTGTGG TGGGCTTTGTGCTGCCCAGTGCTGAGAAAGACATGTGCCTGTCGGACTCCAACAAAGGCATGCTAGGCCT CATTGTGTACCTGGGCATGATGGTGGGGGCCTTCCTCTGGGGAGGCCTGGCTGATCGGCTGGGTCGGAGA CAGTGTCTGCTCATCTCACTCTCAGTCAACAGCGTCTTCGCCTTCTTCTCGTCCTTCGTCCAGGGTTATG GAACCTTTCTCTTCTGCCGCCTCCTTTCCGGGGTCGGGATTGGCGGTTCCATCCCCATAGTCTTCTCCTA TTTTTCGGAGTTTCTGGCGCAGGAGAAACGTGGGGAGCATTTGAGCTGGCTCTGTATGTTCTGGATGATC GGTGGAGTGTATGCAGCTGCAATGGCCTGGGCCATCATCCCTCACTATGGGTGGAGTTTCCAGATGGGCT CTGCTTACCAGTTCCACAGCTGGAGGGTGTTTGTCCTCGTGTGTGCCTTTCCCTCTGTGTTTGCCATCGG GGCTCTGACTACGCAGCCAGAGAGTCCCCGCTTCTTCCTAGAGAATGGGAAGCATGACGAAGCCTGGATG GTACTGAAGCAGGTTCACGACACCAACATGCGAGCCAAGGGCCACCCTGAGCGCGTCTTCTCAGTGACCC ACATTAAAACGATTCATCAAGAGGATGAATTGATTGAGATCCAGTCTGACACAGGAACCTGGTACCAGCG CTGGGGAGTACGGGCTTTGAGCCTTGGGGGTCAGGTTTGGGGGAATTTCCTCTCCTGCTTCAGTCCAGAG TATCGGCGCATCACGCTGATGATGATGGGTGTGTGGTTCACCATGTCTTTCAGCTACTATGGTTTGACTG TCTGGTTTCCCGACATGATCCGCCATCTCCAGGCTGTGGACTATGCAGCCCGAACCAAAGTGTTCCCAGG GGAGCGCGTGGAGCATGTGACGTTTAACTTCACACTGGAGAATCAGATCCACCGAGGGGGACAGTACTTC AATGACAAGTTCATCGGGCTGCGTCTGAAGTCAGTGTCCTTTGAGGATTCCCTGTTTGAGGAGTGTTACT TTGAAGATGTTACATCCAGCAACACATTCTTCCGCAACTGCACGTTCATCAACACTGTGTTCTATAACAC TGACCTATTTGAGTACAAGTTCGTGAACAGCCGGCTGGTGAACAGCACGTTCCTGCACAATAAGGAAGGC TGCCCGCTAGACGTGACGGGGACAGGCGAAGGTGCCTACATGGTGTACTTTGTCAGCTTCTTGGGGACAC TGGCTGTGCTTCCTGGAAACATTGTGTCTGCTCTGCTCATGGACAAGATTGGCAGGCTCAGAATGCTTGC TGGTTCCAGTGTGTTGTCCTGTGTGTCCTGCTTCTTCCTGTCTTTTGGGAACAGCGAGTCAGCCATGATC GCTCTGCTCTGCCTTTTTGGGGGAGTTAGCATCGCATCCTGGAACGCGCTGGACGTGCTGACCGTTGAGC TCTACCCTTCCGACAAGAGGACTACTGCCTTTGGCTTCCTGAATGCCCTGTGTAAGCTGGCAGCTGTGCT GGGGATCAGCATCTTCACATCCTTTGTGGGCATCACCAAGGCTGCTCCCATTCTCTTTGCCTCGGCTGCT CTTGCCCTTGGTAGCTCTCTGGCTCTGAAGCTGCCTGAGACCCGGGGACAGGTGCTGCAGTGAGGGATGG GGGCTGTCTCAGGGGCTTTAGGGATGGCAGGCACACTGTGAGACCAATAATTCCTTTTATCCCTACCCTG CCCTGCTGTCCTGGTCCTCACTCCTGTGTTTGGTGTCTTAGCTGTGTGTGCCTGTGTGCATGTGTGTGAC CCTGATGGGCAGGGACTACAGGGACGGTCCCTTCGTTCCACTTTTGGGATGAGGTACTCCTTACCTGCTG CCACCCTCAACTTTGCACAAGGAGAAGGCTGCGCTGCATCCTTCTCTCCCCCAGTGTTAGCAGGGGGGCT GTTTCCCTGCTCCGAGGGTTCCGGAACTTCTGCCTTCCCCTTCTTTCCCTCCGCCTAGGCCCTGGTGAAC CACAGGTATGGAGTTATAATGGGGGCTGAGGCTTGGACCAAAAGAACTTCTTGAGTGGGAAGGCCTTGGG TGCCCTCTAGCCTCCCAAGGATGCTGGGGAGTAGCAATAAACCTCAGCCCTCTGGCCACCATTTTCACCT CAATTCAGGCTACAAGTGTGAAGCCTGGATTTAATTTATGGAATTAGTTTTCTGAGTTTTATTTATATGT AAGTTCTGAGGCAGCTTAGCTGGACTGTGTGTGGATGTATACATACACTCGTGTGTGTGCGTGCGCATGT GTGTGTGTGTGTACCATGGGGTAGGGGTACCACTCTACTGTCTAATATAAGCCAAGAGTAGTAGTTTCAG TGAACACACACAACACTGTTTTTCTATAGTACCTCCCAGAATCTTGTATCTGTGCTGGGGCTGGAGGCAG AACCCCTAGGCCAGGCTGGGATGAGTCCTGCAATCTTGGGGGACCTGAGGGCACCTGACAAGGACTCACT CTCTCCTTGCTCTAGAGAGGTTCTACCCACTAGCCACAGCCCTCCATCTGACCTGTTCACACAGGCAGTG GACCAGAGGAAAGAAAGGGAGAATAACCAGGCACGTATGGTCAAACCAGCAGGTCCGAAAGCACAAGGAG CTGGGGCAGGAAGCAGGGGTCCGTCCCTAACCCTTCTCAAAAAGGCTGGGTCGTGAGGGACCCCTAATGC AGGGACCAGAAGCCTCAGTTTCCCCGTTTTGCCCTTCCACAGAACAGCCTTGTAGGTAGAGCTGCCCCCG TCCTACCCTACTCTGTGTGGCTGCTTTCTTTGGTACTCTCTCTCCACTCCCAACGTAGCTGTGACGTGTT GTAGTTTTTAGCTGTTTGTAAAATGTTAAAAAAAAAAAAAGTTAAAAGGAAGTTAAACTACCCCCAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6493509 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6493509 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14316 same embryo
	EMAGE:14318 same embryo
	EMAGE:14317 same embryo
	EurExpress:euxassay_004125 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source