EMAGE mouse gene expression spatial database

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EMAGE:14320

Cdh26 cadherin-like 26 ( MGI:2685856)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004137. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004137_01	euxassay_004137_02	euxassay_004137_03	euxassay_004137_04

euxassay_004137_05	euxassay_004137_06	euxassay_004137_07	euxassay_004137_08	euxassay_004137_09

euxassay_004137_10	euxassay_004137_11	euxassay_004137_12	euxassay_004137_13	euxassay_004137_14

euxassay_004137_15	euxassay_004137_16	euxassay_004137_17	euxassay_004137_18	euxassay_004137_19

euxassay_004137_20	euxassay_004137_21	euxassay_004137_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14320_wholemount_strong.wlz

14320_wholemount_moderate.wlz

14320_wholemount_weak.wlz

14320_wholemount_possible.wlz

14320_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14320_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7845
Entity Detected:	Cdh26, cadherin-like 26 ( MGI:2685856)
Sequence:	sense strand is shown >T7845 CATCAGCCCACCTTCTCTGACAAACGAAAGCAGGGCAGGGAACAGAGAACTCAGCAAGGTCAAGTGTCCA GGCTCAGGTACCCAAGAGAGTGATTAGAAGTGATGTCATTGGCTGGAGAGGAGATGCCTGGCTCCTGGTG AGGCCACCAGGCACCAAGGACTGTTCTGTGCCCTCCCTAGCATGGACACCAGAGGCTGTGCCTGGTTGCT GCTGCTGCTGTCATTACCGCAGGGACAGAGCCACCAGCCTCTACATCGATCCAAGAGAAGATGGGTTCTC ACCACCCTGGAACTGCAGGAGGAAGACCCAGGGCCCTTTCCTAAACTGGTTGGGGAGCTCTTCAATAATA TGTCAAACAATGTGTCACTGATATATTTAATCAGAGGACCTGGTGTGGACGAATTTCCAGAGATTGGTTT ATTCTCTATAGAAGATCACCAGAGTGGAAAAATATATGTTCACCGCCCTGTCGATCGAGAAGTGACACCG TCTTTTATGGTGCACTTTGATGCCGTAGATCGATCCACAGGGAAAGTTGTGGATGAATCCCTGATTTTCA ACATTAGAATCAGAGATGTGAATGACCATGCACCCCAGTTTCCTGAGAAGGAATTTAACATCAGTGTGAA AGAGAGCCAAGCCGCAGGTCAACCTATTTTTCAGTTGTTAACGGTCGATTTGGACCAAGAAAACACTCCA AACTCTCAAGTCCTTTATTTCCTCGTCTCTCAAACGCCATTACTGAGGGAAAGTGGCTTTCGGATTGATC TCATCAGCGGGGAAGTGCGACTCTCGGGATGCTTACATTATGAGACTGCTCCTCTGTTCACACTGATCGT CAGAGCAAGTGATTGTGGAGAGCCATCCCTGACATCCACGGCCACCATTCATGTCAGTGTGGAAGACAGC AACAACCACATGCCCACATTTATGGAAGACCGTTATGAGATTCGGATTTCTGAAGGTCAAGTCGAACAGG GTGTGTTGTATCTCCCTGTCCAAGATGGAGATTCTCCATTTACACCAGCATGGAGAACACAATTCAACAT ATGGGATGGCAACGAGGAGGGCCATTTTGATATTGTGACTGACCCTGAGACCAACCAAGGGTTGTTAAGT ATTATCAAGCCTTTGGATTATGAATCTCAAGTAGCCCATAGCCTCGTCGTTGTTGTGGAGAATCAGGAGC AGCTCTTCGTTTGTACGGAGGGCCAGCTGCAGCCATTGAGGAAGGCGATGGCCAGCACCATGGTGAGCGT GCAGGTGCTGGACACCAATGACCCACCAGCCTTTCACCCTCAGAGCTTCATTGTCAGTGAGGAGGATGGA GCCTGGCCCGCCATCCAGCTGGGATACTTCAATGCTACAGACCCAGACAGGGCTGACAGCCAGATAAGAT ACAAACTGGTTCATGATCCAGAAAATTGGGTGACCGTGGATGAGAAGTCAGGAGTGGTGACCACCAAGAA GCAAATAGACCGGGAGTCCCCTCATGTGAATGACAGTTTCTATACAATCATCGTCCATGCAGTTGACAAC GGCCTTCCCCCGCTGACTGGCACGGGGACTCTGATGCTTTTCCTATCTGATGTCAACGACAATGCTCCAA CCCTCCGGCCCCATTCCCGACACTTGGAGGTCTGTGAGTCGGCTGGGAGCCAGCCCCTGCTGATAGAAGC TGAGGATGCAGATCTGGACCCCTACGCTGACCCCTTTACTTTTGATTTGGACAATGCTCAGGGAGACGTG GAAGAGACTTGGATGCTGAGGACAAAGCAGGGGGAAGGCCACTCTGCTGAACTCACAATGCTGAGAAGCG TCCCGCCCGGGGATTACTTGGTGCCACTCTTCATAGCTGACAGACAGGGCCTCGCCCAGAAGCAGACAGT CCACGTGAGAATCTGCTCCTGCCGCAGTGGCTCTGAATGTGAGGAGCCTTCGGACACATGGCTCCTGTGG TGGGCCCTGTCCCCAGTTGGTGCAGCACTTATGGTTCTGTCAGCTGCTCTCCTTTGCCTGCTGCGGTGCT CCTGCACATTTGGACCCAAGAGGCTCAGAGGCTTCATCCCAAGTGACAGTGGCCACCAGACACTGATTGT GTATAACGAGGAAAGCAAAGTCCCTTCAGCCCAGGGTTGTGACACTTTCTTTGAGCCAAGAGGAGTGAAA ACTTTGCTGTCTTCCACCCCGGTCTATCTGGATCGTATGGTACCCAGACAGCAGCCCCTGCAGCTGCTGG AAGGAAGAGTGGTGGAGGCATGGAGCCAGAAGCTCCAGAGTATTGATGTTCTGGAAGGTGACACTGGCTA CCTGCCTCATGTTTACAGAGAGGAGGGCGAGTGTGAAGGAGCTGAGACCCTCAGCTCTCTAACCTTCTTG GAGCAGGACCTCTCCCCCAAGTTGCTGGGCTGCTCGGGCTCAAAATCTACTCCCTCTGAAGCAATGTGTT TTACATCAAGAGTTCCTTCATAAAGGTGTATGAGGGGCATGATTTGGGAAATTGATCACTATTCATGGAC CTCTCCTTTTGTTCTTTATTCTTAGTCACTTCCTAGTCTGCGGTGGTTTGTGTTACAGTCACTTGGGAAA CATCTGGGAGATGTACCTCTGGCATGCCAGTGGGGGTTTCTCTTGTTTGGGTTAAAAGAGATGGGAAGAG CTGTCCACTGTGGAGGGCGACATTCCCTAGGCTGTGGGTGATGCCATTCCCTGGCTGGGATCATGGACTG TGTACATGGAGAAAGCAAGCTGAGCATCAGCGTTCACCACTCTCTGCTTGCTGATCTCAGAAGAACATGA CTGGCTGCTTTAGACTCCTGCCACCTCGACTTCCCGCCCTGATGAACTGTACCCTTGAACTGTGAGCCAG AATAAACCCTTCCTCCTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6337144 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6337144 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14322 same embryo
	EMAGE:14321 same embryo
	EMAGE:14319 same embryo
	EurExpress:euxassay_004137 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source