EMAGE mouse gene expression spatial database

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EMAGE:14336

Ibsp integrin binding sialoprotein ( MGI:96389)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004183. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004183_01	euxassay_004183_02	euxassay_004183_03	euxassay_004183_04

euxassay_004183_05	euxassay_004183_06	euxassay_004183_07	euxassay_004183_08	euxassay_004183_09

euxassay_004183_10	euxassay_004183_11	euxassay_004183_12	euxassay_004183_13	euxassay_004183_14

euxassay_004183_15	euxassay_004183_16	euxassay_004183_17	euxassay_004183_18	euxassay_004183_19

euxassay_004183_20	euxassay_004183_21	euxassay_004183_22	euxassay_004183_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14336_wholemount_strong.wlz

14336_wholemount_moderate.wlz

14336_wholemount_weak.wlz

14336_wholemount_possible.wlz

14336_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14336_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

humerus

strong

regional

strong expression: see section 01 02 03 22 23

fibula

strong

regional

strong expression: see section 01 02

tibia

strong

regional

strong expression: see section 01 02 23

femur

strong

regional

strong expression: see section 04 05 06 20 21

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 15 16 17 18 19 20 21

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 16 17 18 19 20

premaxilla

strong

regional

strong expression: see section 10 11 12 15 16

axial skeleton cervical region

strong

regional

strong expression: see section 08 09 moderate expression: see section 10

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 20 21 22 23

clavicle

strong

regional

strong expression: see section 04 05 06 07 08 09 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7856
Entity Detected:	Ibsp, integrin binding sialoprotein ( MGI:96389)
Sequence:	sense strand is shown >T7856 GAGAACAATCCGTGCCACTCACTCGAGCCAGGACTGCCGAAAGGAAGGTTAAGAAGAAATTGCAAAATGA AGACTGCTTTAATTTTGCTCAGCATTTTGGGAATGGCCTGTGCTTTCTCGATGAAAAATTTCCATCGAAG AATCAAAGCAGAGGATTCTGAAGAAAACGGGGTCTTTAAGTACCGGCCACGCTACTTTCTTTATAAGCAT GCCTACTTTTATCCTCCTCTGAAACGGTTTCCAGTCCAGGGAGGCAGTGACTCTTCAGAAGAAAATGGAG ACGGCGATAGTTCCGAAGAGGAGGGGGAGGAAGAGGAGACTTCAAACGAAGAGGAAAACAATGAAGATTC TGAGGGGAATGAAGACCAGGAGGCGGAGGCAGAGAACTCCACACTTTCCACACTCTCGGGTGTAACAGCT AGCTACGGAGCAGAGACCACACCCCAAGCACAGACTTTTGAGTTAGCGGCACTCCAACTGCCCAAGAAGG CTGGAGATGCAGAAAGCAGGGCTCCAAAAGTGAAGGAAAGCGACGAGGAAGAAGAAGAAGAAGAGGAAGA GGAAGAAAATGAGAACGAAGAAGCAGAAGTGGATGAAAACGAGCTGGCGGTCAACGGCACCAGCACCAAC TCCACGGAGGTGGATGGCGGGAATGGCAGCAGCGGAGGAGACAACGGAGAAGAAGCCGAAGCAGAGGAGG CAAGCGTCACTGAAGCAGGTGCAGAAGGAACCACAGGGGGCAGGGAACTGACCAGTGTTGGCACCCAGAC AGCTGTCCTTCTGAACGGGTTTCAGCAGACAACCCCACCGCCCGAAGCCTATGGGACCACCTCGCCACCG ATCAGAAAAAGCAGCACCGTTGAGTATGGGGGAGAATACGAACAAACAGGCAACGAATACAACAATGAGT ATGAAGTCTATGACAACGAGAACGGGGAGCCTCGTGGCGACACTTACCGAGCTTATGAGGATGAATACAG CTACTACAAGGGGCATGGCTATGAAGGCTACGAGGGTCAGAATTATTACTACCATCAGTGAAGCCCCCTC CCCGGGGGGAATTTCCCCATTTGAATTCCCCATTCTGTCTTATCATCTGGCTACCATTTTCAAAATTCAA CTCAGGAAGGTGCAATATAACAGATGTGCATTTTATAGTGTGGAATGGTGCTACAGCCCCAGAGTGATTT CCGCAAATGCTTTTGTTTGTAGTGGGCTTCTTCTTTAAAAAAAACAAATTTCCCAGGTGTGTCATTGAAG AGTCTTAGTAAATCAGGGCTATTGATCAAGCAGCACACAGATCTATGAGCTGGGACTAGATGGATACTTT GGGATTATAGCTCTATAGTGTTTGTGCTATTGCTAACTGCGCAAACATACCCTGTATAAGAAGGCTCCTA ATGAGAGATTTATTAACAACACTATATATGATGTGTGACGTTCCTTTATCACACCCCCTATCTAATACTG TATTAGGTTTAATTTGCATCCTCATGTATTTTATGTAACCTGTGCACATGCATTTTTATCACAAATAAAT ATGCAATCTTTCACATGTGTTATAATTAAGAAGAAAGAGATTTAGAATGAGTGTGGGTTTAAGGAGCAGG GAACAATTCAGCTAAAAAGCAACACTTATTTCCATCAGGGTTTTAAACTCAGAGGATCTGGAAGCATTCT TTCTTCAATACATCTGAATGGCTAAGGTTTGACTAAGGAGGGCACAGTCACTGTATACAGGAGGAAGTAA ATGTCTCCCCTTAAAGTTGCCAGATTTAACAAGCATGAATACATGTTATAAGATGCCCACTTCAGTTTGA ATTCCAGATAAACAATAAGTGGCTTTTAATACAACCCCTCCCCCCATGAATGATTTGTCTCTGTGAAACA TCTGGAACAAACTTATGCTAAATAAATGACTTATTCTTTATCTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6391115 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6391115 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14338 same embryo
	EMAGE:14337 same embryo
	EMAGE:14333 same embryo
	EMAGE:14335 same embryo
	EMAGE:14334 same embryo
	EurExpress:euxassay_004183 same experiment
	MGI:4825496 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source