EMAGE mouse gene expression spatial database

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EMAGE:14337

Rtp2 receptor transporter protein 2 ( MGI:2685451)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004166. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004166_01	euxassay_004166_02	euxassay_004166_03	euxassay_004166_04

euxassay_004166_05	euxassay_004166_06	euxassay_004166_07	euxassay_004166_08	euxassay_004166_09

euxassay_004166_10	euxassay_004166_11	euxassay_004166_12	euxassay_004166_13	euxassay_004166_14

euxassay_004166_15	euxassay_004166_16	euxassay_004166_17	euxassay_004166_18	euxassay_004166_19

euxassay_004166_20	euxassay_004166_21	euxassay_004166_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14337_wholemount_strong.wlz

14337_wholemount_moderate.wlz

14337_wholemount_weak.wlz

14337_wholemount_possible.wlz

14337_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14337_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 weak expression: see section 08 13 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7855
Entity Detected:	Rtp2, receptor transporter protein 2 ( MGI:2685451)
Sequence:	sense strand is shown >T7855 GCTTTTTGGACCACAGAGACTGCCTGGGCTGCATCTATCTAAGCTTTCTGTTCTTCCTTGGGCTGAGTTC ACCCTTCTATCCCAGCCAACAGGACCATGTCCACCAGCCTGACCACTTGTGAGTGGAAGAAGGTCTTCTA CGAGAAGATGGAGGTGGCCAAGCCAGCGGACAGCTGGGAGCTCATCATAGACCCCACCCTCAAGCCCAAT GAGCTGGGCCCTGGCTGGAAGCAGTACCTGGAGCAACATGCCTCAGGCAGGTTCCACTGTTCCTGGTGTT GGCACACATGGCAATCTGCCAATGTCGTCATTCTCTTCCACATGCACCTGGACCGTGCCCAGCGTGTTGG CTCAGTGCGCATGCGCGTGTTCAAGCAGCTGTGCTATCAGTGCGGCACGTCGCGGCTGGACGAGTCCAGC ATGCTGGAGGAGAATATCGAGGGCCTGGTGGACAACCTCATCACCAGTCTGCGCGAGCAGTGTTACGATG AGGATGGTGGCCAGTACCGCATCCACGTAGCCAGCCGGCCAGACAGCGGATTGCACCGCAGTGAGTTCTG CGAGGCCTGCCAGGAAGGCATCGTGCACTGGAAGCCCAGCGAAAAGCTGCTGGAGGAGGATGCCGCCTAT ACCGATGCCTCCAAGAAGAAGGGCCAGGCTGGTTTTATCTCCAGCTTCTTCTCATTTCGTTGGTGCCTGT TCTGGGGCACCCTCTGCCTGGTCATTGTCTACCTGCAGTTCTTCCGAGGCCGCTCTGGCTTCCTTTAGTG GTAGGGGTGGGGAAAAGGGACTCATGGGATTGAAAATGAGAAGGATTTGGTCTCCTCCTGAGTCTGCTAC GGGTTCTATCTATGACTCTGGTTATCTGCACACTGCTCTCTAGTCTGCACTAGGAGAAACTTAGTGGTCC TTCCACTAATTTAATAGCAACGGAGGGAAAGTAGAAGGAATAGGGATTTGGAAGTTTGCTGGAGTTTAGT GAGTCTGAAATCTGCAGCTGCCACCTCCAGCCTTTACTTTACATTTCTAGCCTTAGGTCCCATCTGGACA CTGTCGCTTTCAGCTTTATGTGCCCTGCATGGTTCCCCTTGGAGGGAGCAGAGCCACCTCCAGCCTCCAT CGAATACATAGGGACAGCCTGCTTCTTCCATTCTGAAATGCTGAGGATTTCACTCAGGCGCTAGGGCTGG GATGAGGATAGAGGTGGGGGGTGGTGGAAGTGGGGATGGGCTGAGGGGGTTGGAATGGTCAAGGGCGCAT TGTCCAATGGATTAGCTATTGGCCATATGTGGCTGCTCTGATTAAGATTAATTAATATGAAATAAAAGTG TTCCTTAGTAGTTCCAAACTCAGTCAGGCTTATACAGAATTTGTCCCCATAGAGGCCATTGCTCAATGGA AGACAAGGGTGACTTCTAGGAAGATGAACACCAAGGGTTTGGGCATCAAGTGCTCCCCAGAGGTCTTCCA CCCTCTGCTTTGTCTACCCTGGGAACCCCTACGCAGCGCTGAACAACAGGACAGGAAAAACTAGGCGCCA GCTTGCATCTCTTGCTAAAATCTGTTTTGTGTCTAGAAATAGTTTGCCAAAACTCTGTCAAGAAAATAGG TCATGTCTCAGAGGGTCAGTAAAGCCAGGGTGTCTGACCACCGCATCCGAAGGAGGAAATGCGGCTGAGG ATGGCTGTGAGGGCAAGGAAGCTAGAATAGTTGGAGAGAAACGAGGCCGGGAATATCTCCAGAAGCCCAG TTTCCTCGATAAACAGTGATTTCTGCATTTTCATGTTAAATTGGAGTGACTGGTCCCTGACTCGGGGTCC CTTCCCAACCCGCTGCAATGATTTTATCTCCTCAGGATTGAGGGAGTAGGTTATATTAGTTTCCAAGACT TGTTTCCTCTTTAGTCCATGGAAATCTCATTTCTACATCCCTAGAGCCAGCCTTCCATACTTCACTACCA AAACATTGGCTGCTTTTCTGTTTGTGTCTGCCACATTTTATAGTCCTCTTTCTGTAATTGTTCTGTTCAT TGAAAACGTGGCTGTCACTCTAATATTTTCCTTACATGTATTCTGGTCATATTCAAAGATATATCACCTC TTCTGACCCCCCCAGCAACTGGTTCTCTCTTCTCTGAATGATCCCTTTCCTGCTTTTGTATCTATGTTTA AAAGTCTCCTTTTTACACGTGAGGGAACACAAATGATATTTTTCTCTTTTAGCCTAACTGATTTAGCTTA ACATGATCTCCAGTCCCACCCACTTCCCAGAAAGTGACATTTTTATTTTTCTTTATAACCAAATATATTC CATTTTGTACACATGACATACTTTTTCATCCATTGATCAATCTGTTAATGGGTATATCATCTGATTTCAT AACTTGGCTATTGTAAATAGTGTCATAATAAATGCAGATATGTAGGTAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6390582 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6390582 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14338 same embryo
	EMAGE:14333 same embryo
	EMAGE:14336 same embryo
	EMAGE:14335 same embryo
	EMAGE:14334 same embryo
	EurExpress:euxassay_004166 same experiment
	MGI:4827833 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source