EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14343

Scara3 scavenger receptor class A, member 3 ( MGI:2444418)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004188. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004188_01	euxassay_004188_02	euxassay_004188_03	euxassay_004188_04

euxassay_004188_05	euxassay_004188_06	euxassay_004188_07	euxassay_004188_08	euxassay_004188_09

euxassay_004188_10	euxassay_004188_11	euxassay_004188_12	euxassay_004188_13	euxassay_004188_14

euxassay_004188_15	euxassay_004188_16	euxassay_004188_17	euxassay_004188_18	euxassay_004188_19

euxassay_004188_20	euxassay_004188_21	euxassay_004188_22	euxassay_004188_23	euxassay_004188_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14343_wholemount_strong.wlz

14343_wholemount_moderate.wlz

14343_wholemount_weak.wlz

14343_wholemount_possible.wlz

14343_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14343_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 17 18 19 20 24

body-wall mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 14 15 16 17 18 19 20 24

cochlea

strong

regional

strong expression: see section 06 07 08 09 16 17 18 19

mandible

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 16 17 18 19 20

upper jaw skeleton

strong

regional

strong expression: see section 13

maxilla

strong

regional

strong expression: see section 10 12 13

clavicle

moderate

regional

moderate expression: see section 06 07 08 09 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7887
Entity Detected:	Scara3, scavenger receptor class A, member 3 ( MGI:2444418)
Sequence:	sense strand is shown >T7887 CGCGCAGAAGGGACCATGAAAGTGAGATCAGCTGGCAGTGACAGGGATGTACTGTGTGTCACTGAAGAGG ACCTGGCTGGTGAAGACGAGGACATGCCATCCTTCCCATGCACCCAAGAGGGCCGGGCAGGACCCCGCTG CAACCGCTGCCAGAAGAACCTATCTTTGCACACATCAGTGCGGATTTTGTACCTCTTCCTCACTCTGCTC CTGGTGGCCGTGGCTGTGCTGGCCTCTCTGGTTTTTAGAAAGGTGGACTCTCTCTCAGAAGATATCTCCT TGGCCCAGTCCATCTATAATAAGAAGCTTGTGTCAATGCAAGAAAATCTTCAAGGACTAGACCCAAAAGC CCTGATCAATTGTTCCTTTTGCCGTGAGGCTGAGCAGCTGGGGCAAGAGATCAGGAAGGTCCAGGAGGAG CTGGAGGGGCTTCAGAAGATGCTGCTAGCCCAGGAGGTCCAGCTGGACCAGACCTCCCAGACCCATGAGC TGCTCTCCACCAGAAGCAGTCAAATCTCCCAGGAGATGGGCAGTTGCTCCTTCTCCATTCACCAGGTCAA TCAGTCCCTGGGACTCTTCCTGGCACAGGTGAGGGGCTGGCAGGCCACCACAGCAGGCATGGACATCACC CTGAAGGACCTCACCCAGGAGTGCTATGATGTCAAGGCTGCTGTACACCAGATCAACTTCACGGTGGGCC AGACTGCTGAGTGGATCCACGGGATCCAAAGGAAGACAGATGAGGAGACCCTGACCCTGCAGAAGATAGT CACTGACTGGCAGAACTACACCAGACTCTTTGGGGGTCTGCGAACCACCTCTGCCAAGACTGGCGAAATA GTCAAGACCATTCAGACCACCCTGGGGGCCTCCTCACAGCGCATTAGCCAGAATTCTGAGAGCATGCATG ACTTGGTTCTGCAGGTCATGGGCCTACAGCTACAGCTGGACAACATCTCTTCCTTTTTGGATGACCATGA GGAGAACATGCATGACCTCCAATACCACACCCGATATGCCCAGAATCGCACAGTGGAAAGGTTCGAGTCT CTGGAAGGACGTATGGCTTCTCATGAGATTGAAATCGGCACTATCTTCACCAATATCAATGCCACCGACA ACCACGTGCACAGCATGCTCAAGTACCTGGACGACGTGCGGCTGTCTTGCACACTGGGCTTCCACACCCA TGCTGAAGAGCTCTACTACCTGAATAAATCCGTCTCCCTCATGCTGGGCACCACAGATCTGCTCAGGGAG CGCTTCAGTCTGCTCAGTGCCCGGTTAGACTTCAATGTCCGAAACCTCTCCATGATCATGGAGGAAATGA AGGCTGTGGACACACACCACGGAGAAATCCTTCGCAATGTCACCGTCATCCGAGGTGTCCCCGGCCCTCC AGGACCAAGAGGACTCAAAGGAGATACAGGCGTGAAAGGACCTGTTGGTAGCAGAGGACCTAAAGGGGAT CCTGGCAATCTGGGACCCCCAGGACCTCAGGGTCCTCAGGGGCAGCCTGGGGAGCCAGGACCTGTGGGAG AAAGAGGGCCAGCTGGCCCCAGAGGCTTTCCAGGCCTCAAAGGCTCAAAGGGCAGCTTTGGCACTGGAGG ACCAAGGGGGCAGCCAGGCCCCAAGGGGGATGTGGGGCCCCTAGGGCCAGAGGGGCCCCCAGGGTCTCCA GGACCTTCAGGGCCTCAGGGAAAGCCAGGAATTTCAGGAAAGACAGGGTCACCAGGCCAGCGAGGGGCCA CAGGGCCTAAGGGTGAACCAGGCATCCAGGGTCCCCCTGGACTACCTGGACCTCCAGGTCCACCAGGGAA CCAGAGCCCCTACTGAAAAAAGTCTTTGGCCTGATGCTGCCATGCAGGTTGGAGAGGTGAGGGTCAGAGA AAGCAGCCAGAAGGGTTGCTACAATCATTGTTCTTTGATCCTAATACGAGGGTGTCTCTTAGAGCTGACT GCATAGCCTTAAGCTTCCCAATAATGACTGTACCATAGGAAAACAGCTCACCTCCCCCTACTTTTTTCTG CTGACTAAAGGGGAGTCTATTGGGTTTCTATGGCTGGTTAGAAGGAAAGGCTGTGAGAGCCTGCTGGTGG GGGAAGGGATAGCTACCTGGGGAAGAATGTCTTAGATCTCTTGGATGGTAACTACTCTGGTCTCTGCAAT GACTTAACACCCATGCGAGCCAAGTCTTGAAGAAAAAAAAAATCCAGGGAAACTTTTGGGGCATTATAAA TGAAACTCCAGGGATAGCAGCCTCCCCTCTTTCCCCCTCCCTCGACACATAACAGTCACGGTAGAATCAT GTGGAGCTGCCTTGATCCACACAGGGCAAGAGAAGTCTGCCTGGCTGGCATTCCCAGGCCTTATGCATGG ATACTGACCCTGACAAGTTCAGAGAGTTTTTATTCACATGTCCAGAAAATTCCTCTTTTCAGTCTTACCT CTGCATCTCAAGAGTGGCCTGGAAGAAACCCAAGCAGAGTTTCATCGGCAGGTCAAGAACCTACAGGTCC CAGAGCACTCTGCTTGACTTCTATCCAGCTCCCAGGAAGACCACAGGGCTGGAGGTGGGAGGACATATCA GGGGAAAACTCCCCTCTCCAAAGCCACCACTTGTTCAACCTACTCTGCCTTGTCTTACTCCCAGTAATAG CCAAGGAGGAAGAAGCTGGTAACACGGCAGCTCTTGCCTCCTCCTCTTGGTTGACCTGAGACAGATGCAC AATGACCTCACCCCACAATGACCTAAGATGGCTGGAGTTCTTTCCCACATGAACAGCAAGCTGGACCAGG CATGCGAAAGGCTGAACGTGCACAAGGAACCTTAGATGTTACTTCTCGTCTTTCATGAACTTCTTGAATA CACTGGCAGAAAGGGATGCTGGGAAAGGCCTGCATTTCCTTGACTCATTTTGTAGAGCTGCCCTGATCCT TCATACTCCAGCAGACTCAAACACTCCTGGGCAGAGGCTAGAGCTGTGTCCTCTCTTGCCCTGCCTCAGA GACCTGTCTTCTTCTTCCTGTTCAAAGGCTCTGTGCTACCTTCTTCATCAATATCAGTGTGCAACTGGCT TGCACGACCCATCTACCCCTGCTGTATAATTGTCCTGTCTACATATGAAAATATCATGCAACACAATATC GCTGACCCAGTAAAGAGCTGTTTCCAGTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6508271 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6508271 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14339 same embryo
	EMAGE:14340 same embryo
	EMAGE:14341 same embryo
	EMAGE:14342 same embryo
	EMAGE:14344 same embryo
	EurExpress:euxassay_004188 same experiment
	MGI:4827886 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source