EMAGE mouse gene expression spatial database

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EMAGE:14344

Tlk1 tousled-like kinase 1 ( MGI:2441683)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004209. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004209_01	euxassay_004209_02	euxassay_004209_03	euxassay_004209_04

euxassay_004209_05	euxassay_004209_06	euxassay_004209_07	euxassay_004209_08	euxassay_004209_09

euxassay_004209_10	euxassay_004209_11	euxassay_004209_12	euxassay_004209_13	euxassay_004209_14

euxassay_004209_15	euxassay_004209_16	euxassay_004209_17	euxassay_004209_18	euxassay_004209_19

euxassay_004209_20	euxassay_004209_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14344_wholemount_strong.wlz

14344_wholemount_moderate.wlz

14344_wholemount_weak.wlz

14344_wholemount_possible.wlz

14344_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14344_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7884
Entity Detected:	Tlk1, tousled-like kinase 1 ( MGI:2441683)
Sequence:	sense strand is shown >T7884 CCGCCCGGCGCGCTCCCTCGGCGGCGCGGCGCTCCCCTCCCAGCCCTCGCGCCCGCGCTCGCCCTCAGCG CGGCCCCCGCCATGACGGAGGCGGGTGCCGGTGCCGCCGCCGTCGCAGGGGGGGAGTCGGGCTCCCAGAA AGTAGCTTGATGAGTGTCCAAAGTAGCAGTGGAAGTTTGGAGGGGCCGCCATCTTGGTCCCGGCTCTCCA CGTCTCCAACCCCGGGCTCGGCGGCGGCGGCCAGGTCCCTGCTGAATCACACGCCGCCATCCGGGAGGCC CAGGGAAGGTGCAATGGATGAGCTTCACAGTCTGGACCCAAGAAGGCAAGAATTATTGGAAGCAAGATTT ACTGGAGTTGCTACTGGGAGTACTGGGAGTACTGGGAGTTGCAGTGTTGGAGCAAAAGCTTCAACAAATA ATGAAAGTTCTAATCACAGTTTTGGAAGCTTAGGATCTCTAAGTGACAAAGAATCAGAGACACCAGAGAA GAAACAATCAGAGTCATCCAGGGGAAGAAAGAGAAAAGCAGAAAGTCAGAATGAAAGTAGTCAGGGAAAA AGTATCGGGGGACGTGGCCATAAGATCAGCGACTATTTTGAATACCAAGGTGGAAATGGCTCGAGTCCAG TAAGAGGCATCCCTCCAGCAATCCGTTCTCCGCAGAACTCACACTCACATTCCACTCCTTCCTCATCTGT TCGACCGAATAGCCCTTCTCCTACTGCATTAGCTTTCGGGGACCACCCTGTTGTACAACCAAAGCAGTTA TCCTTTAAGATCACTCAAACTGATCTTACAATGCTGAAATTGGCAGCATTAGAAAGTACTAAAAATCAAG ACCTGGAGAAGAAGGAAGGCCGGATTGATGATCTGCTCAGGGCCAACTGTGATCTCAGGCGGCAAATAGA TGATCAACAGAAATTACTTGAAAAATACAAAGAACGATTAAATAAGTGCATATCTATGAGCAAGAAACTA CTTATTGAAAAGAGTACACAAGAAAAATTGTCTAGCAGAGAAAAGAGTATGCAAGATCGGTTGCGCCTTG GGCACTTTACCACAGTTAGACACGGCGCTTCCTTTACAGAACAGTGGACAGATGGTTTTGCATTTCAGAA TCTTGTGAAGCAACAAGAATGGGTGAATCAACAAAGGGAAGACATTGAACGGCAAAGAAAACTTCTAGGC AAACGCAAACCTCCCACAGCTAATAATTCTCAGGCACCTGCTACCAATTCTGAAGCCAAGCAAAGAAAGA CCAAGGCAGTCAATGGAGCGGAAAACGATCCCTTTGTTAGGCCAAATTTACCACAACTGCTGACTCTGGC AGAGTATCATGAACAAGAGGAAATTTTTAAACTTAGACTAGGACATCTCAAAAAGGAAGAGGCTGAAATC CAGGCGGAACTGGAGCGTTTGGAGAGGGTCCGGAACCTTCACATCCGAGAGCTGAAGAGAATAAACAATG AGGATAATTCACAGTTCAAAGATCATCCAACGTTAAATGAAAGATATTTATTACTTCATCTGCTTGGGAG AGGCGGCTTTAGTGAGGTCTATAAGGCATTCGACTTGTATGAGCAAAGATACGCTGCTGTGAAGATCCAC CAGCTTAATAAGAGCTGGAGAGACGAGAAGAAAGAAAACTACCACAAACACGCTTGCCGAGAGTATCGAA TACACAAGGAACTGGACCACCCCAGAATAGTTAAACTCTATGATTACTTCTCCTTGGATACAGATACGTT TTGTACAGTGCTAGAGTACTGTGAAGGCAATGACTTGGACTTCTACCTGAAGCAGCACAAGTTGATGTCT GAGAAAGAAGCTCGGTCTATTGTGATGCAGATTGTGAATGCCTTACGCTATCTCAATGAAATCAAACCTC CTATTATACATTATGACCTTAAGCCAGGAAATATCCTTCTGGTAGATGGAACAGCATGTGGAGAAATCAA AATCACTGATTTTGGTCTGTCCAAGATTATGGATGACGATAGTTACGGTGTTGATGGGATGGATCTGACT TCCCAGGGGGCAGGCACTTACTGGTATTTACCTCCTGAGTGCTTTGTAGTTGGAAAAGAGCCACCGAAGA TCTCCAACAAGGTTGACGTGTGGTCAGTTGGGGTCATCTTCTTCCAGTGCCTCTATGGTAGAAAACCATT TGGTCACAATCAATCTCAACAAGACATTCTACAGGAAAACACAATACTGAAAGCCACAGAAGTCCAGTTC CCTGTAAAACCTGTTGTAAGCAGTGAAGCCAAGGCCTTCATAAGACGCTGTTTGGCTTACCGAAAAGAAG ATCGATTTGATGTGCACCAGCTGGCAAATGACCCATACCTTCTGCCACACATGAGAAGATCAAATTCTTC AGGAAACTTACATATGTCTGGGCTGACAGCAACTCCCACACCTCCTTCTTCAAGCATAATTACTTACTGA CTCCTCTAAGATTGGCATGACCTCTTCTCTTTGATTGGCTTCCAGATGCACACTTGAATTTGACTGCATT GGACTGTTTGTGGTATTTTTACATGGACATGGTTAAGAAGTGTTTGTGAACTAAGCTCTCCAAGGAGTCC TTGTGTGAGAGTGGATCATGGACAGTGACTAAGTACACACTCACTCCTCACTGCCCTGGAGCAGCACGAA ACAGGAATACCATGTTACAGTAGAACAAGGGGACTCTGTAAATAACGTTTATAATACTTAAATATATTTG ATTGTTACCAGAGAGTTGTAATATGAAGGGTAGTTTTTCATTATTTAAGAACACATGGAAAAATGAGGAG ACATTTGTAACCCGGGAGCTACAGTGCCTGGTACAGCGTCAGCACTCGGCAGCTGATCTACAGAAACCAA AGCAAGAACTGAGTGACAGCAGCCGCTGTGTTTATAACAGAATGTGAGAGTTAGGGTCTTTGGACAGTTG GAGACTTTGTCATTTGCCTATTCTGACTTTTACCAAGTTGTACCTCGAGATCACGTGTCAGTGGTCCGCC GCCCTACCCCCGACCCCTTAACCGGTTATGTTAAAGGCAAGCTGTCGTTTCTGGACACTTTACACCTTTA ACACAAAGAGCTGCCTATTTCAAATCAGTTGAAGTTACTAAATGATAGTATTTCCAGGGCTTTCAGTGGG TTAGATTAACAGGCGTTTTTCTTTTGTTTTCAGGCCTGGTTCATTAAAACAGAAGACAGTGTGTAACCAA AAGCAGGCCAGTTGTATGCAGGATAATGATGAATTCCCTTCTGGCTCTATTGTAAACTACTGTACAGATG TCGGATTACAATTACTGAGTTTCAGCAGCTTATTCTTGTACAAATGTTTAAAAATACGGCTTTTCTAATT GGATATTGTATTTTTTTTAAGTCTTCTTGAAGGCGCTTTAATTGCTGCTAAATGATATTGCTTTTTTGCT ATAAAAAATTGAATGACCTTGAAGGCACAAGTTGACTGTACATCATAGAGAGATATTAAAAGCAGGCACT CGTTAACAATCAAGGCATGTGCAGTGATCCATGCTGGGCATATTGAGTTCTTCAAATCAGTTTTTTAGGT TCTGGAGCTGTGGAACACATAGAAACTAGATTTATAAATTGTTCATGGTTATTCTACATTTCTAGAAAGT TCTTAGCATCAAGGTGGGATGATAGTCCCATAGAATGTTTCTCGTGGTTTGTACAGATTTGTTAAAATGT GAACATTTTCTAACTACCTTGTGTGGTAGAAACCAATATTTTTTTGGGGATGCTGTATTTCATTCTTGAA AGGATTTTTTTTTCTTTATTGTCAAATTCATAATGCCGAGACTTACATGTAAGTTGTCCATGGTGCAGAC AACAGGCTATCCCTGCCAGCCGCCCCTAACATGTACAGTTACACTGTGGCTCTTACCTGGTGTTGCAAGC CATTTCGTTTGTTTGTTTTCATTGTACATAGACACACTGTCTGTTTAAGATGCTGCTGGAATTATAGAGA ATGTAGCCAAACAGTAATAAACAATGACAGTAAAAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6507968 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6507968 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14339 same embryo
	EMAGE:14340 same embryo
	EMAGE:14343 same embryo
	EMAGE:14341 same embryo
	EMAGE:14342 same embryo
	EurExpress:euxassay_004209 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source