EMAGE mouse gene expression spatial database

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EMAGE:14348

Nap1l5 nucleosome assembly protein 1-like 5 ( MGI:1923555)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004121. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004121_01	euxassay_004121_02	euxassay_004121_03	euxassay_004121_04

euxassay_004121_05	euxassay_004121_06	euxassay_004121_07	euxassay_004121_08	euxassay_004121_09

euxassay_004121_10	euxassay_004121_11	euxassay_004121_12	euxassay_004121_13	euxassay_004121_14

euxassay_004121_15	euxassay_004121_16	euxassay_004121_17	euxassay_004121_18	euxassay_004121_19

euxassay_004121_20	euxassay_004121_21	euxassay_004121_22	euxassay_004121_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14348_wholemount_strong.wlz

14348_wholemount_moderate.wlz

14348_wholemount_weak.wlz

14348_wholemount_possible.wlz

14348_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14348_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

forebrain

weak

regional

weak expression: see section 05 06 07 08 09

diencephalon

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

telencephalon

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19 20

hindbrain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

midbrain

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5620
Entity Detected:	Nap1l5, nucleosome assembly protein 1-like 5 ( MGI:1923555)
Sequence:	sense strand is shown >T5620 GGACCAGCCGCAATCACAGCTGCAGGACCTCTCTGGACCAGCTCTATTGCAGACTGCGCAACCACCAGAC CACTGCGGCAAACAAGCCCAGCTGAGCCGAGCAGTAGCCATGGCCGACCCCGAGAAGCAGGGACCCGCTG AGAGCCGCGCCGAGGACGAGGTAATGGAAGGCGCCCAGGGTGGCGAAGATGCCGCGACCGGTGACAGTGC CGCTGCTCCCGCGGCCGAGGAGCCCCAGGCCCCCGCGGAGAACGCGCCCAAGCCCAAAAAAGACTTTATG GAGAGCTTGCCCAATTCCGTGAAATGCCGGGTTCTGGCGCTCAAAAAGCTGCAGAAGCGCTGCGATAAGA TCGAGGCCAAGTTTGACAAGGAATTCCAGGCTCTGGAGAAGAAGTACAACGATATCTACAAGCCCCTGCT CGCCAAGATCCAGGAGCTCACCGGAGAGATGGAGGGCTGCGCGTGGACCCTGGAGGGAGAGGATGATGAA GATGACGAGGAAGAGGACGACGAGGAGGAGGAGGAGGAAGAAGAGGCTGCAGCTGGCGCAACTGGGGGTC CCAACTTCGCCAAGAAGTGAGCACAGCAAGCTGACAGGTGATAAATCCCCTGCCCCTTTTATTTCCAATG TCGGCAGACTTTAAAAGGCTCAGGATTTGCCCAAAATAAAATGTGAATCTATTCTGACATTCCTAAAATT AAATTGAATCAAGTAGATTCTGGCTAGCCCGTTTCTAATCTGACTTTTATTTGTGTAAAACAGATGCTAA AAGACGTTTTTTAGAAAAAAAAAAAAGCCTAATTAAACTTTTTTTTTTCTCCCTGCTCTTCTGTGAGAAC TGGACTTGAGACTGTTGAAAGGTTGTCAGTTAGATGGGAATTAAAAGTGCGTCACACGTTGAAATCCATT CATCACACTACACCAGAACATCCAAGCTAAGACAGAACTCTTCTCAATGCTTAATTCTTCAGTTTCTTTA CATTTTCCAGCGCAGAGGAAAAGGAACCCAGGAACGACGTCATCTTTAAGACTTTTGCTTTTGCAAACCC AGACATCAGCTTTACACTCCAGAGGAGCCTTGGCATGGAGCAAGCCTGGACTGACAGCATTTACTGATTA TGTGGCTAGGAAAACTGCCATTTCAAGTTGTGAAAAAATGTTTTAAATATTTGCATTTACAGAAAGAACA TGGTTTCAAAAATGAGGGTGTATTCCATGTATAATATTGTCAACACGTGTTCATCTGTTATGGTCCCGTG TTACCTGTTTTCTTGGTAGTGTTTGTTTACAAAATCGTAAAAAATTACCCCAAATGTTTTAAAGTGTTAA ATTACCTTATAGCATTTTAGAAATATAATTTACTTGAAGAGATGTAGAATGTAGCAATTCTGTAAAGCAT GTGTATCCAGTGTTGCCTAGTTTGACCTTTGTGAAGTCTTTTTGTCTTGTAGCTTTTAGCAAGTAGCTGT GAAAACCATCAGAACTCCTCAATGAAGCTAATGTTTGGAAAAAGTATATACTTGAAGAACAAACCCAAGT GTGTACCCCCAACCCCAGCTCAGAAATAGAAAGGGTTTAAGTTTGCTTGTATTAGCTGTGCCTTCATTAT TTTGCTATGTAAATGTGACTTAATAAATGGTGCAAAATTAAATTTTATTGCTTGAGGACAGACGGGCATA AAGGAAGGGAAAAGACATTTAATGTAAAGGCCTCTAGCTTCTTTGTGGGTTTTGAGTTGTCTGTGAACCA ATGTTCTGTAAAGAAACACAAACTTAAAGTTGTTTACCACTGTGGTGTTAACAAAACAGTATTTTCAAAA ATAAAAAAATCCTTGTTATTCTGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6491916 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6491916 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14350 same embryo
	EMAGE:14347 same embryo
	EMAGE:14346 same embryo
	EMAGE:14349 same embryo
	EMAGE:14345 same embryo
	EurExpress:euxassay_004121 same experiment
	MGI:4826596 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source