EMAGE mouse gene expression spatial database

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EMAGE:14358

Ccnt2 cyclin T2 ( MGI:1920199)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004177. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004177_01	euxassay_004177_02	euxassay_004177_03	euxassay_004177_04

euxassay_004177_05	euxassay_004177_06	euxassay_004177_07	euxassay_004177_08	euxassay_004177_09

euxassay_004177_10	euxassay_004177_11	euxassay_004177_12	euxassay_004177_13	euxassay_004177_14

euxassay_004177_15	euxassay_004177_16	euxassay_004177_17	euxassay_004177_18	euxassay_004177_19

euxassay_004177_20	euxassay_004177_21	euxassay_004177_22	euxassay_004177_23	euxassay_004177_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14358_wholemount_strong.wlz

14358_wholemount_moderate.wlz

14358_wholemount_weak.wlz

14358_wholemount_possible.wlz

14358_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14358_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7890
Entity Detected:	Ccnt2, cyclin T2 ( MGI:1920199)
Sequence:	sense strand is shown >T7890 CGCGGAGTCCCGGAGGCAGGCGGGGAGGGGGCGGGCTCAGGGCGCGGGCGGAGGAGGAAGTGTCATGGCG TCGGGCCGTGGAGCTTCTTCTCGCTGGTTCTTTACTCGGGAACAGCTGGAGAACACGCCGAGCCGCCGCT GCGGAGTGGAAGCGGATGAAGAGCTGTCGCATCGCCAGCAGGCGGCCAACCTCATCCAGGACATGGGGCA ACGTCTCAATGTCTCTCAGCTTACAATAAACACTGCGATTGTTTATATGCACAGGTTTTATATGCACCAT TCCTTCACCAAATTCAACAGAAATATAATATCACCTACTGCATTGTTTTTGGCTGCAAAAGTGGAAGAGC AGGCTCGAAAGCTTGAACATGTTATCAAAGTAGCACACGCTTGTCTTCATCCCCTAGAGCCACTCCTGGA CACAAAATGTGATGCTTATCTTCAGCAGACTCAAGAACTGGTTTTACTTGAAACCATAATGCTACAAACA CTGGGTTTTGAGATCACCATTGAACACCCACACACAGATGTGGTGAAATGCACCCAGTTAGTAAGAGCAA GCAAGGATTTGGCACAGACATCCTATTTCATGGCTACCAACAGTTTGCATCTTACGACCTTCTGTCTTCA GTACAAACCCACAGTGATAGCATGTGTATGCATTCATTTGGCTTGCAAATGGTCCAATTGGGAGATTCCT GTGTCAACAGATGGAAAGCACTGGTGGGAGTATGTGGACCCCACGGTTACCCTAGAGCTGCTAGATGAAC TAACACATGAGTTTCTACAAATATTGGAGAAAACGCCTAGTAGGTTGAAGAGGATTCGAAACTGGAGGGC TATGGCTAAGAAACCAAAAGTAGATGGACAAGTATCAGAGACACCACTGCTTGGTTCATCTTTGGTCCAG AATTCTATTTTAGTAGATAGTGTCACTGGTGTACCTGCCAACCCAAGCTTTCAGAAACCATCAACATCAA CGTTCCCTGCTCCAATACCTCTAAATTCAGGAAGTACTTCTGTTCAAGATAGCCGTGCATCTGATAATTT ATCAGTGCTAGCAGCAGGAATGCCCAGTACCTCATACAGTTTGTCATCACACCAAGAGTGGCCCCAGCAT CCAGATTCAGCCAGGACAGACCCAGTGTACACACAGAAACAGGAGGCCACTCTCTCTGGGAGCCAGTACA TAAGCTTCCAGCAGGGACCTTCAATGGCACTGCATTCTGGATTACATCACAGGCCGGACAAAGTTGCTGA TCATTCATCAGCTAAGCAAGAATATACTCACAAAGCTGGGAGCAGTAAACACCATGGTCCCATACCTGCT ACTCCTGGAATGCTTCCTCAGAAAATGTCTTTAGATAAGTACAGAGAGAAGCGGAAGCTGGAAACCCTTG ACGTGGACACAAGGGATCACTATCTAGCTGCCCATGCAGAACAGCAGCACAAGCATGGGCCGGCTCAGGC AGTCACGGGCACTTCTGTCACTTCTCCCATCAAGATGAAGCTCCCCCTCACCAACTCGGACAGGCCTGAG AAACACGTGGCAGAGAAAAAGGAGAGGAGTGGATCACTGAAGCTCCGCATTCCCATCCCCCCGCCTGACA AAGGTCCCAGCAAAGAGGAGCTGAAGATGAAAATCAAAGTGGCATCCTCGGAAAGACACAGCTCTTCTGA TGAAGGCAGTGGGAAGAGCAAGCACTCAAGCCCACACATCAGCAGAGACCACAAGGAGAAGCACAAGGAG CACCCTGCCAACCGCCACCACAGCAGCCACAAGTATTTGCACATGCACAGTGGAGGCAGTAAGCACACAG CGGATGGAATGCCTCCCACGGTGCTCAGGAGTCCTGTGGGCCTGGGCCCTGAAGGCGTCTCCTCCGCCTC CAGTGCAAGGAAGAAGCTGCATAGCAGCGAAGCCTCCCACAACCACCACTCCAAAATGAGCAAAAGTTCC AAAAGTGCAGGTAGTTCATCTAGTTCTTCCTCTGTTAAGCAGTATCTATCCTCTCACAGCTCTGTTTTTA ACCATCCCTTACCCCCTCCTCCCCCTGTCACATACCAGGTGGGCTACGGACATCTCAGCACCCTCGTGAA ACTGGACAAGAAACCAGTGGAGCCCCACGGTCCTGAGGCCAATCACGAGTACAGTACAAGCAGCCAGCAT ATGGACTACAAAGACACATTCGACATGCTGGACTCGCTGCTAAGTGCCCAAGGAATGAATATGTAACCTT TGTTTAGGTCAACTTTTCCTTGCCTTTTTAATTTAAAAATGGTTGGAATGGAAAACGCCCTCCTGGTCCG GCGGTGGTGCCACTGCTTCCATATTTGTAAGTGCTGCTTTGTTCTTCATTCTGAAAGAAGAGATGATAGT AAACAAGCCTATCTCCACATACGATAGTGTTACCAGGACTGTAACCGCATGGAAGATGCAGCTGAGAACA TTAGGATGAATGGCTGGCTGCTGCTAGGAGCCTAAGCTGCTTCAAGCTTCTCTTATTTATGATTTGAATA TTGTAGCTGTTTTTTGTTGTTGCTTGGCTTTGAATGAGTGTCAGTTGTTTTCTTTTGTGTATTTATACTT GTATGTATGGTTTGCATGTCTCAGTGATAAAGGGATAAAACAGTATACTGACAGCTGTTTACAAGAAAGT GGAGAAAAATGTACATACATTTTTGTATGTTTAGATATTACCATAAATACTCAGGATTGGAGCTGCTTGT AAGAATAACAATATACAGAATAACTTTATTTTCTCTCGTCAGAGTCCATCACTAATCTAAAACACAAGTG GCACTTTTTTTATGTTAACCTTAATTAAACTCTAGGCCTTACTGGAAGCCGCTGAGGGGACACTTCACTA CTAGACGGGAATGTGGTGCCATGGGTGGTCCTTAACACTGGGAAGCACTCAGTTTGCCTTCAGAGGTCCT GACCAGATTGGCTGCAGAATCAGCCGTTGATTTCTGAAGGCTTGAAGAGGAAAACACAATAAAGTTTACA TACTCTTGATGTGAAGTGCATTTAAATGTTTGTTGGCTTGTTGCAGTGCTGTAGAAAGCAGCTAATAATT GGTGTGAGGATGACTGTGACTTTGCTCACAGCAGCTCCCAGTGAAGATGAGTCCATTTCTTCAGGAACGC TGACACTACAGATTTTAAGAGTGAACAGGGGTCACTTTTCCTTTAGCATTGCAGTGACACCATAGTCTTG AATATACTCTTCCCATGGAGAGAGAGAGTGAGTGTGTATGTGAGAGAGAGTGTGAGTGTGTGGGTATGGG TGTGAGGGAGAGATACCATATTTCTTTTTCAGGTAAATGCAGTCTTCCTTATAAAAATGAAATTAAACCT ATGCTCTCTTGATTCTTTTATATTCTAACAATAAATAAAAAAGAAAAGATTGCTGACTGTGAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6509024 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6509024 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14362 same embryo
	EMAGE:14359 same embryo
	EMAGE:14357 same embryo
	EMAGE:14360 same embryo
	EMAGE:14361 same embryo
	EurExpress:euxassay_004177 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source