EMAGE mouse gene expression spatial database

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EMAGE:14361

Hmgxb4 HMG box domain containing 4 ( MGI:1918073)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004182. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004182_01	euxassay_004182_02	euxassay_004182_03	euxassay_004182_04

euxassay_004182_05	euxassay_004182_06	euxassay_004182_07	euxassay_004182_08	euxassay_004182_09

euxassay_004182_10	euxassay_004182_11	euxassay_004182_12	euxassay_004182_13	euxassay_004182_14

euxassay_004182_15	euxassay_004182_16	euxassay_004182_17	euxassay_004182_18	euxassay_004182_19

euxassay_004182_20	euxassay_004182_21	euxassay_004182_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14361_wholemount_strong.wlz

14361_wholemount_moderate.wlz

14361_wholemount_weak.wlz

14361_wholemount_possible.wlz

14361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7891
Entity Detected:	Hmgxb4, HMG box domain containing 4 ( MGI:1918073)
Sequence:	sense strand is shown >T7891 GCGGCCGTCGGCGGCGCTGAGGCGGGATCCGAGCGAGCCGAGTGAAGACCTGGTCCTGTAGACGGGAAGG AGCCTGGACACAGTGACACAACACATTCTCAAAGGCCCTGCAGGACCACCATGGCTTATGATGACTCCAT GAAGAAAGAAGATTGCTTTGATGGTGATCACAGCTTTGAGGACATAGGACTTGCAGCTGGCCGAAGCCAA CGAGAAAAGAAGCGGTCTTACAAAGACTTCTTAAGGGAAGAGGAAGAAGTCGCCGCGCAGGTCAGGAGCT CTTCCAAGAAGAAGTCAAAGGACAGTGATTTGTTCTTCTTGGGGACGGACACACACAAGAAGAAGAGGAA GCACTCCCCTGACGATTACTACTATGGAGACATTTCGACTTTGGAATCATCACAGAAGAAAAAGAAGAAG TCTAGCCCACAGTCTCCGGACACAGCAATGGACCTGTTGAAAGCTATCACCTCCCCACTGGCAGCGGGTG CCAAGTCCTCTAAGAAGGCAGGGGAGAAATCCTCCAGCTCTTCAAGCCATCCAGAGAGCAGGAAGGAGCA CCACAAGAAGAAGGGCAATGGCAGCAGTGGAGAGCTCTCCCTGGATGACAGCAATGCCCACAAATCCAAA AAGATGAAGCCACTCTATGTGAACACAGAGACACTGACTCTTCGTGAGCCAGATGGCTTAAAGATGAAAC TCATTCTCTCACCAAAGGAGAAGGGAAGCAGCTCAGTGGAAGAGGAGGCTTTTCAGTACCCTGCCCAACA AGCTTCGCTGAAGAAGCCCTCGAAGAAACTAGCTCGGGACGAGCAAGGTGCTTTACTCCTAGGGCACGAG CTACAGAGCCTCCTGAAAACGGCCCGGAAGAAGCACAAGTCATCCTCAGACTCACACTCCTCTCCTGGCC CAGAAGGCTGTGGGTCTGACGTCTCCCAGCTTCCAGAACCCCACAGTGCCAATCTCGACATCACAGGGCT TGACCCAATTCTAGTAGAGTCCGAATCGTCCTCAGCTGGGGAGCTGGAGGCCGGGGAGCTGGTGATAGAT GACTCTTACAGGGAGATCAAGAAGAAGAAGAAGTCAAAAAAGAGCAAAAAGAAGAAGGACAAAGACAGAC ACAAGGAGAAGCGGCACTCCCGGACCAAGAGAAGCTCTGCCAGAGAGCACGGTACTGCTCGGGAGCACGG GCTGATACCTGGCCCTGCCTCCAGTGTCCCCACCCTGCCACTTCCTGCTCTACACACAGATGGACACGGG GAGAAGAAAAAGAAAAGGGAAGACAAAGACAGAGACAGAGAGAGGGGAGAAAAGCCGAAAAAGAAGAACA TGTCTGCCTACCAGGTATTCTGTAAGGAGTACCGGGTAACCATTGTGGCTGACCATCCAGGTATAGATTT TGGGGAACTTAGTAAAAAATTGGCTGAAGTATGGAAGCAGTTGCCAGAAAAAGACAAACTGATCTGGAAG CAAAAGGCTCAGTATCTGCAGCACAAACAGAACAAAGCTGAAGCCACAACTGTGAAGAGAAAAGCATCCA GCGCAGAAGGTTCCATGAAAGTCAGAGCCTCTTCTGTAGGAGTACTGTCACCCCAAAAGAAGTCCCCACC CACCACAATGCTTTTGCCAGCCTCACCAGCCAAAGCTCCTGAAACAGAGCCCATTGATGTCGCTGCTCAT CTCCAGTTGCTGGGAGAGTCCCTCAGCCTCATTGGACACCGCCTACAGGAGACAGAGGGCATGGTGGCAG TGTCTGGAAGCTTGTCAGTGCTCCTAGATTCCATTATCTGTGCTCTTGGCCCTCTGGCATGCCTGACCAC ACAACTACCTGAACTGAACGGCTGTCCTAAACAGGTCTTGTCAAACACGCTGGACAACATTGCATACATT ATGCCTGGACTGTGACTTCCAAGACGCCTGGGACAGAAGACTGTCTACCCCTTCGCTAGTCTATGTATAG ATGACTGTTCCAGACCCTCCACTGACCTCTGGGTGTAGGTTTTAAATTTTTATAATCTATATATACGTAT ATAATGTACAGTATATACATAGGATATAACTACTTTGCTTTTAATAATTTTATGAAATGGCAAAGGTTTA GCTAAAAGGAAACTTTAGCATGAATGTGGGCTTGGAATTATTTTTTTGTGAAAAAAAAATCTGCCTTAAA AAAAATCAGTGTAATTTGGGGACTAGGGGCTTATTCTGGGTGCCATGTATATCTCCTCATAGACTGCTAA AATGTGATTTTTTTTTTTTTTCCAAGCTTGGGTTTACCTCCAGACCCATCACTGACCATTTGCCTTACCT GTCTTATAATCAGAAATGTAGTAAAGATCTTGGGAGGAGGCCAGCTGACTACAAACTTTTGGACATGGAG TAACCTGGGTGGGGAGGTGGAGCTTAGGTGGACTGGGTAGGGGACTCATTCCAATTCCTACTAAAGAGAT ACCTGTGTCTCCAGTTTTATTGTAACCACGGTTCCACAGAGTCATCGGTACCATCTATAGCCTCAGCTAT GTGATTCTTGGCAGCAGAGAAATAAATACCTTGCAGAAACCAAAGCTGTTGGTGCCACCTGTTGAACCTG CTTGTCTGGCCAGCAAGGCTTGGTGTATAAAAACCAAAGCTGCCATCTTCCACTAGCAGCATTGGTTGCC CAACAAATCCTGAGGATTCTGCTGCTTAGCTGTTTGGTGGTTCTTGGTTCAGCTCTGGTTCCCCTGGTGG GTACATTGTGCTCTGCATTCCACACAGAGCTGGATCACAAAGAAGTTCTTGGTTCTCAGGAGGGTTATCT ATATGTGCGTGTGAGTGTATATTTTAATTATGTGTATTTAACTCTTTAGAATTTCTTAGATTTTAATTTA TCCTGTAAATTTCTGTATATATAATTTAATAACAAGCCTCCACCAGCAGGAGCATGTGGAGCATACAGAT TTGTCATTTCTTAATGTCTGTCTAAAAGCTCCAAGACGTCCATATGTTAAGGTGTTGTGACTCAGAAAGG AACTTCATGGCCCTCAATAATGTTAGGAAAGTGAAATAGAAAGGAATGTCACCATTCCTAATTTCCCTTA TTCCCAAAGACCCAGACCTGCTTCCCCATTAGAGAGAAGATAGCTGAGTGCTCCAGGGTTGCTTGGGAAC TCGGTGAGAGATTCCCTTCCCTGCCAAAACCAAGCTGAGTTAATAACCATGCTTTCTTCTAATGGCCTGG ATGGAACGAGGCAGCATCACAGTAAACCTTTGGGGACCATGTAGCCAGCTGGCATCCTAGGGAACTCCTT CCCATAGCCCGACTGCTGTCACAGCTAAGCTTCTCACAGGACTGTGTCAGCTCAGAGCAGTTTTGAGATT TAATCTGAGAAATAGGGAAAGGAACAGAAATATGTGGGAACTGAAATCAAGCAGAGCAGAAATTTCCATT TGAGTTAACATGTCAGAACCTCATTTGACCCTGCAGAAGTTTCCTGTGAGAAATGGTATCAAGGAGAGGC CAGGGAAGACATGTGTGGTAGAGGAAGCAGCCAGTCCTCCATTGCCCTTGCTACAGACAGTAGTGAACTC CTAAGGAGAACCGGCTTTTTCGGGTTTGTTTTCCTCTGAAGCCTAGCCCTGTTGTATTTGTATTAAAGAT TTGTACACATTTGTATAATAATCTGTACCTTGTGGTATTTGGTACTATTAGAGGAAAAATTTTGGATTCA GGCCTTCTTGCTGTTTTTATATCATTGTTTTATTTTGTTTTTTTAAATAAATCCTAGTGGTTTGATCCAA ATCCTATTACAGTTGTATAAAGAAACAATATTTTGTACTTATATTAAAGATCTCATTTTTAATAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6509446 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6509446 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14362 same embryo
	EMAGE:14359 same embryo
	EMAGE:14357 same embryo
	EMAGE:14358 same embryo
	EMAGE:14360 same embryo
	EurExpress:euxassay_004182 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source