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EMAGE:14381

Mpdu1 mannose-P-dolichol utilization defect 1 ( MGI:1346040)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381
"Pseudo-wholemount" of euxassay_004268. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_004268_01 euxassay_004268_02 euxassay_004268_03 euxassay_004268_04
EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381
euxassay_004268_05 euxassay_004268_06 euxassay_004268_07 euxassay_004268_08 euxassay_004268_09
EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381
euxassay_004268_10 euxassay_004268_11 euxassay_004268_12 euxassay_004268_13 euxassay_004268_14
EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381
euxassay_004268_15 euxassay_004268_16 euxassay_004268_17 euxassay_004268_18 euxassay_004268_19
EMAGE:14381 EMAGE:14381 EMAGE:14381 EMAGE:14381
euxassay_004268_20 euxassay_004268_21 euxassay_004268_22 euxassay_004268_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14381Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14381_wholemount_strong.wlz
14381_wholemount_moderate.wlz
14381_wholemount_weak.wlz
14381_wholemount_possible.wlz
14381_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14381_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7217
Entity Detected:Mpdu1, mannose-P-dolichol utilization defect 1 ( MGI:1346040)
Sequence:sense strand is shown

>T7217
ATGGCGGGGGAGGCCGACGGGCCGTTCAAAGGGCTGCTGGTGCCAATTCTTTTACCTGAGAAATGTTACG
ACCAGCTCTTCGTGCAATGGGACTTGCTTCATGTTCCCTGCCTCAAGATTCTCCTCAGCAAAGGCCTCGG
GCTGGGCATCGTGGCTGGGTCACTTCTCGTCAAGCTGCCCCAGGTATTTAAACTCTTGGGAGCCAAGAGT
GCAGAAGGACTGAGTCTCCAGTCAGTAATGCTGGAGCTAGTGGCACTGACCGGAACCGTGGTCTACAGCA
TCACCAACAACTTCCCCTTCAGCTCTTGGGGTGAAGCGCTGTTCCTGACACTCCAGACCGTTGCCATCTG
CTTCCTGGTCATGCACTACAGAGGAGAGACCGTGAAAGGAGTCGCTTTCCTTGCCTGCTATGCCATGGTC
CTGCTGGCGCTGCTCTCCCCGCTCACGCCTCTGGCTGTAGTCACTCTGCTCCAGGCCTCCAATGTACCTG
CCGTGGTGGTGGGGAAGTTGCTTCAGGCAGCCACTAACTACCGCAACGGACACACAGGCCAGCTTTCAGC
CATTACAGTGTTTATGCTGTTTGGGGGCTCCTTGGCCCGAATCTTCACTTCTGTTCAGGAAACTGGAGAC
CCCCTCATGGCTGGAGTCTTTGTGGTCTCTTCTCTCTGCAATGGCCTCATTGCTGCCCAGGTCCTCTTCT
ACTGGAACGCAAAGGCTCCCCACAAACAGAAAAAGGAGCAATAGAGCTGAGCTCGCTTCTAGAACAATTC
CATTTCCACTCATCCTCAGAGTCCTCCCCACATGACTTGATCTGCGGGTGCAGTGTAAGCACCCTCCATT
CCTCTAACTGCAGCCTCCTGAGCCAGGGTTGTGGGAGCAGTAAAGCTTGTGTAGGCCCTGCCTTCACTCA
CTCACTTCCCCAGATCCCTCCTTGTGAGCAGCTGGTTTACTGGAGCAGATGCTCTGGCCCTGTCCACTTA
CCTTCCTTTTGGACCAAGGACAGAGCCTAGAAAACTGTGGTTGGAAGCTAGTGGCTGCTTCCCTGTGCTG
CCCGGGTGGGATGGTCTATTGGAGCCCAGTTCTGGGGGTGAGGGAGAGTGACTCAGATAAGGGCCCTGGG
GTTGGGTGGGGAGGTTCCTGCCGAGGCAGGTCCTGGCGGAAGGGTGTGTGAAGGCGCTGGTTGCTGCTGT
AGGGAGAGAATACAAATGGGACAATAAAAGACTCCGAAACATGGTTTTAATTGTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4159355 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4159355 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14379 same embryo
 EMAGE:14378 same embryo
 EMAGE:14380 same embryo
 EMAGE:14377 same embryo
 EMAGE:14376 same embryo
 EurExpress:euxassay_004268 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS