EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14384

Ptgis prostaglandin I2 (prostacyclin) synthase ( MGI:1097156)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004313. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004313_01	euxassay_004313_02	euxassay_004313_03	euxassay_004313_04

euxassay_004313_05	euxassay_004313_06	euxassay_004313_07	euxassay_004313_08	euxassay_004313_09

euxassay_004313_10	euxassay_004313_11	euxassay_004313_12	euxassay_004313_13	euxassay_004313_14

euxassay_004313_15	euxassay_004313_16	euxassay_004313_17	euxassay_004313_18	euxassay_004313_19

euxassay_004313_20	euxassay_004313_21	euxassay_004313_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14384_wholemount_strong.wlz

14384_wholemount_moderate.wlz

14384_wholemount_weak.wlz

14384_wholemount_possible.wlz

14384_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14384_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 03 04 17 18 19 moderate expression: see section 01 02 20

diaphragm

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04

foot mesenchyme

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

tongue

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15

mandible

strong

regional

strong expression: see section 05 06 07 08 09 10 14 15 16 17 18 19 20

maxilla

strong

regional

strong expression: see section 06 07 08 09 10 11 12 16 17 18 19 20

genital tubercle of male

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19

clavicle

moderate

regional

moderate expression: see section 06 07 08 15 16 17

tail mesenchyme

moderate

regional

moderate expression: see section 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7910
Entity Detected:	Ptgis, prostaglandin I2 (prostacyclin) synthase ( MGI:1097156)
Sequence:	sense strand is shown >T7910 CATCCACGTTTTCCAGTCGGACCCCTAGAGTCGGGGCCTTTGGGGCTGGCTGGGTTGAGAATCCTGCGGT CCGGTGTTGGTGGCGGTGACTTGTTGCCACCCTGCAGCCGCACACCCTCGAGCCATGTCCTGGGCTGCTC TGCTCGGCCTCCTGGCCGTGCTATTGCTGTTGCTGCTGCTGCTGAGCCGCCGGCGCGCGCGGAGACCTGG TGAGCCTCCGTTGGACCTGGGCAGCATCCCCTGGCTGGGCCATGCCTTGGAGTTTGGGAGAGATGCTGCC AGCTTCCTTACCAGGATGAAGGAAAAGCACGGTGACATATTTACTGTGCTGGTGGGGGGCAGATACGTCA CTGTTCTCCTGGACCCACACTCTTACGACACAGTGGTGTGGGAGCTGCGTACACGGCTGGACTTCCATCC CTATGCCATCTTCCTCATGGAGAGGATTTTTGATCTGCAGCTTCCAAATTTCAACCCCAGTGAGGAGAAG GCCAGGATGAAGCCGACGCTCATGCACAGAGACCTGCAGGCGCTCACAGAAGCCATGTATACCAACCTTC GCACGGTCCTGCTGGGTGACTCCACAGAAGCAGGCAGTGGCTGGCAGGAGACAGGTCTCCTTGAGTTCTC CTACAACGCCCTGCTCAGCGCCGGCTACCTGACCCTATATGGAGTGGAGGCCTCACCACGCACCCACGAG AGTCAGGCCCAAGATCGTGTCCACTCAGCGGATGTTTTCCATACCTTCCGCCAGCTGGATCTGCTGCTCC CCAAACTGGCTCGAGGCTCCTTGTCAGCGGGGGATAAAGACCATGCATGCAGTGTCAAAAACCGCCTGTG GAAGCTGCTGTCCCCAGCCAGGCTGGCCTCCAGGGCTGACCGGAGCAGCTGGCTGGAGAGTTATCTGAGA CATCTTGAGGAGATGGGGGTGTCAGAGGAGATGCAGGCCCGGGCTCTGGTGTTGCAGCTCTGGGCCACAC AGGGGAATATGGGTCCCACGGCCTTCTGGCTCCTTCTTTTCCTCCTCAAGAATCCGGAAGCCCTCGCCGC TGTCCGTGCAGAGCTGAAGCACACGGTCTGGCAAGCAGAGCAGCCTGTTTCACAGATGACCACACTCCCA CAGAAGATTCTAGACAGCATGCCTGTGCTAGACAGTGTGCTCAATGAGACACTCCGGCTCACGGCTGCCC CCTTCATCACCCGTGAGGTCATGGCAGACCTGGCCTTGCCTATGGCAGACGGGAGGGAATTCTCTCTTCG ACGTGGTGACCGCCTTCTCCTCTTTCCCTTCCTGAGTCCCCAGAAGGACCCAGAAATCTACACAGAGCCT GAGGTGTTTAAATACAACCGATTCTTGAACCCAGATGGATCTGAAAAGAAAGATTTTTACAAAGATGGGA AACGGCTGAAGAATTACAACATGCCATGGGGCGCAGGGCACAACCAGTGCCTGGGGAAGAGTTATGCCAT CAACAGCATCAAACAATTTGTCGTCCTACTGCTGACTCATTTCGACCTGGAGCTGGGCAGCGAGGACACA GAGGTTCCTGAGTTTGACCTCAGCAGATATGGCTTCGGTCTGATGCAGCCAGAGGAAGACGTGCCCATCC GGTACCGTGCCAGGCTGTGACCTATGGAGCAGACTCACCTGGGGCCTTCTGACTTCCTGTTGTTCCTCTA GAATGCTATGTCCAGCGGAGGGGACACTGAGGACAGTGAGAGCAGGAAAAGCTAATAAAAGCTCTGTATC TCGCTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6512164 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6512164 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14382 same embryo
	EMAGE:14385 same embryo
	EMAGE:14383 same embryo
	EurExpress:euxassay_004313 same experiment
	MGI:4827509 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source