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EMAGE:14396

Flywch1 FLYWCH-type zinc finger 1 ( MGI:2442638)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14396 EMAGE:14396
euxassay_004249_01 euxassay_004249_02

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14396Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14396_wholemount_strong.wlz
14396_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14396_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T7149
Entity Detected:Flywch1, FLYWCH-type zinc finger 1 ( MGI:2442638)
Sequence:sense strand is shown

>T7149
CCACGCGTCCGGGTAAAGGGCACAAGGTCAAAGACAGCCCAGGTAGGCACCCAGGTACCTGAGCAGGCAC
AGTGGGATTTCAGCCTCTTCATTCTCCGGCGTACCCAGTTTCAGTACCTCTCTCCTTACTTATCCTAGTA
GCTGTTCACCTTGTAGTACTGATGCTGGAAGGGCTCTGTGCTGGACATTGATGAGGCTTCTAAGGTAGAA
ACTCAGTAACTTAGCATGTTAGTCAGTGGCAGCCCCAGAGAGGCACTTCCTGTGCAGTCAGTTTACACGT
GACAGGGAGGTTTGGGTGGGGCTGTCGGGAAAGGTCCTGATAGAGCATGGCCCAGGTCCCTTTCCTCTTC
AGCCTCTCAATCCCACGGTACCAAGCAAGGTCCATATCCCCGAGCTCCATCCCTGCTCCATTCGTTAGAG
CCCCAGGCCCAGGAGCCCCCTCCACTGACTGCCTTGGCTCTTGTCTTAGGACGGAGGCTCTGGATTCCCA
CATTCTGAAGAGAGACCCATCTGCCAGAGGGCTGAAGGTGGCCTTGAGGGCCATGCTCTGGGTCTCAGGA
TGCCCCTGCCTGAGCCCAGTGAGCAGGACTGTGAGAGCCTGAGAGCTGGCCAGGAGCCATCTGTTGGAGC
CAGGAAGCCTCAAGAGTCCTCCAATCTGGTCCCGGCCAGGGATAAAGAGAGACCCAAGCCGACAGATGTC
GCGTCCCAGGAGACGTCTAGCACTGCAACCCTACCGAACAATACTCTCCAGGTTGCACCAGTCAAGAAGC
AAGGACGGATAATTCACCGGAAACGTAGTAGGGTAGATGCAGTTCCTCCCCAGCCTCTGGAGTTCCTGAA
GACCCCATTTGGGGGCCGCCTTCTGGTGCACAAGTCATTCCTGTACAAGCAAGAGAAGGCTGTGGGGGAC
AAGGTGTACTGGAAATGCCGCCAGCACTCAGAGCTAAGCTGCCGGGGACGGGCCATCACCCGTGGCTTTC
GAGTCACAGAGATGCGGGACCACTGTCACCCACCTGAGAAGGAGGGCCTGGACAGGAAAAAGCGCCACAG
GGGAAGGCCGCCCAGCTCAGCCCTGCCTGAGGGCGCTGAAGTCCAAGAAGATGAAGTGAGCCTATGGTTG
TACCCTGTGGAGCCAGAACCTACTCCTCAGCCAAGCACTGAAACCCCAGAGGAGGAGCAGGGGTACCGGT
CCCTGGCACTGCAGAGCCTGCCTCCCAAGAAACGCCCTACACCAGGAGTGGTACGGTATCGGCCTCTTGA
GTTCCTGAAGACTTGCTATGGGGGTACCTTCCTGGTTCACCAGTCCTTCCTCTACAAGCGGGAAAAGACG
GTAGGAAGCAAGGTGTACTGGACCTGCCGGGAACATGCAGTCCATGGCTGCCGTAGCCGAGCCATCACCC
AGGGCCAGCGTGTGACAGTGATGCGCAGCCATTGCCACTCACCTGACATAGAAGGCTTGCAGGCCCGGCG
ACAGCAGGAGAAGACCATAAAGAAGATACAGGCAAGGCGAATTGGGGCTGGGGACTTGGAGGATTGCGAT
GACATAGAGGACTCACTGCTGCAAGGAGTGGACAGCCTGTTCTACCGAAGAGGGCAGGGCACACTGACTC
TCAGTAGGTCCAAGTCCAAGTCCAAGTCCAAATCCAGGTCCAAGTCCAAGTCCAAGTCCAGGTCCAGGTC
CAGAAAACGAGCAAAGAAGCAGCAGGAATCTTCGCAGGAGCCCCCCGAGGAGGACCAGGATGTAGACCCC
AGAGGCCCAGAGTTCCTAAAAACCCCTCTAGGGGGCAACTTCCTGGTGTATGAGTCCTTCCTCTACAGAC
GGGAGAAAGTGGCTGGTGAGAAGGTGTACTGGACCTGCCGGGACCAGGCACGCATGGGTTGTCGCAGCCG
CGCCATCACTCAGGGCAGGCAGGTGACTGTGATGCGAAGCCACTGTCATCCACCAGACCTGTTAGGATTG
GAGACTCTGAGGCAGCGGGAGAAACGCCCAGGCCCCTCTCAGTGGGATGGCCCAGAAGGGCCTGAGTTCC
TAAAGACCCCTCTAGGAGGCAGCTTCCTGGTATATGAGTCCTTCCTCTACAGACGGGAAAAAGCAACTGG
TGATAAGGTGTACTGGACCTGCCGGGACCAGGCACGCATGGGCTGTCGCAGCCGAGCTATCACACAGGGC
CAGCGGGTGATGGTCATGCGCAGGCACTGCCACCCACCGGACATGGGTGGCCTGGAAGCCCTTCGGCAGA
GGGAGAACTTCCCTAACCTGACCCACTGGGAAGGCCCAGAGCCCCTGCAGCCACTGGAGTTCCTGCGGAC
ATCCCTTGGAGGCCGCTTCCTGGTGTATGAGTCCTTCCTGTACAGGAAGGAGAAGGCTGCTGGTGAGAAA
GTGTACTGGATGTGTCGAGACCAGGCTCGGCTGGGCTGCCGCAGCCGAGCCATCACCCAGGGCCGGCGGG
TCATGGTGATGCGGAGCCACTGCCACCCACCTGACCTGGCTGGCCTGGAGGCCCTGCGGCAGCGGGAGAA
GGCCCCCAGTGCAGCCAAGAAGAAAAAGAAGAAGAAGAAGAAGAAGAAAGGTATACACTGAGTGACCAGG
GCTTTTGCCTAACTTGTGCATTTTCAGAACCTTTGCCTCTCATTTCCTCTGGTCCCTTCCCATTGCCATG
AAACTGTACCTGCTTCTTCCTGGGCCTTTGCCCAGCAATCTTTCCAAACCCATCTTCCTCTCTTATTCTG
AGACAGCTCAACACTTAGAGCTGGTTGTCTCTTGAAAAATTTAATGAAAAATATAGACTACCACAAAAGA
AAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4021754 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4021754 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14401 same embryo
 EMAGE:14400 same embryo
 EMAGE:14398 same embryo
 EMAGE:14397 same embryo
 EMAGE:14399 same embryo
 EurExpress:euxassay_004249 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS