EMAGE mouse gene expression spatial database

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EMAGE:14398

Eif1ad eukaryotic translation initiation factor 1A domain containing ( MGI:1917110)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004240. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004240_01	euxassay_004240_02	euxassay_004240_03	euxassay_004240_04

euxassay_004240_05	euxassay_004240_06	euxassay_004240_07	euxassay_004240_08	euxassay_004240_09

euxassay_004240_10	euxassay_004240_11	euxassay_004240_12	euxassay_004240_13	euxassay_004240_14

euxassay_004240_15	euxassay_004240_16	euxassay_004240_17	euxassay_004240_18	euxassay_004240_19

euxassay_004240_20	euxassay_004240_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14398_wholemount_strong.wlz

14398_wholemount_moderate.wlz

14398_wholemount_weak.wlz

14398_wholemount_possible.wlz

14398_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14398_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7199
Entity Detected:	Eif1ad, eukaryotic translation initiation factor 1A domain containing ( MGI:1917110)
Sequence:	sense strand is shown >T7199 GCTTTTGTTTCTCCAGCGGCCCTTCCGGTCTGTGGGATCGAGACCTCCGAGGAGAATGCGTTTTCCTGCC CCGCACTCGGAACTTAGGCTTGCCTCACTTGATGGAGGGAAGAGTTAAAGGGACGGACGAAGAGGGGGCG GGAAATCCGGAGGACCTCTGAGCGCCCCGGAATCGCAATTCCCGGCGCGGTTTCCTCAGGTCTGTCACCT CAGACCGGCAGACGGGAGGGAGGAACAACGGAGGATTGGGGCCGAGCGTCCAGACCGGCGTTACCGTCGG GGCCACCTGCAGGTCACAGTTGTTTGCTCTTTGGTCCCTCGCTGTCGAGGCGCGTCGTGGACGAGCTACT GTCGCCGTCAACCTGGAAAGCGGAGCGTGCTGGTGTGGCGCCGAGCGGGGCGAGGTGGCACTTTTTCGTA TAAGGCTGCTTCTCTTGAAAGCAGTCCTGGCTTCTAAGGCTGGCATCCAAATAATTCTGGAAGAAGAACT GACAGTCTGTGCACCCTAACAACCCTGGACAGAAACCAGCATGTCTCAGGCCAGCAAGAGGAAGCATGTG GTGCAGGAGGTGCTGGGGGAGCACATGGTGCCTTCTGACCACCAGCAAATAGTGAAAGTCCTCAGGACTC CTGGGAACAATCTGCATGAGGTGGAAACAGCACAGGGGCAGCGCTTCCTGGTGAGCATGCCCTCCAAATA CCGCAAGAACATCTGGATCAAGAGAGGGGATTTCCTCATTGTTGACCCTATTGAAGAAGGAGAAAAAGTG AAGGCTGAGATCTCTTTTGTGCTCTGCAAGAACCATGTCCGCTCTCTACAGAAGGAGGGTCACTGGCCTG AGGCCTTCTCTGAGGTAGCTGAGAAACAGAACAATATGAACAGAGAGAGTCAACCAGAACTCCCTGCTGA GCCACAGCTGTCAGGAGAAGGGTCAAGTTCTGAAGATGATTCTGACCTCTTTGTCAACACCAATCACAGA CAGTATCATGAGAGTGAGGAGGAGAGTGAAGAAGACGAGGAGGAGGAGGAGGAGGCAGCCTGAGCCCCAG GACCCACTTCTCTATTTGCTCAGGGACTACCCCTTGGCTTTTCTGGGCTTGGACATCCCCAGGGTGCCCT GCAGATCTTTTCCTTTGGATGAGGACAAAGATGGCCCCTTTCTGAACTGACCTAAAAGAATTAAACCCCT GGTGGGTGATGACTTACACTGGTGTCTGAGTGGCTCTCTGTGGAGGGAAGCTCATGGTGAAGTCTCTTCG GTGGTGGAAGCCTTGGTCACGTATGAGGAGAATGGAAAGGAAATGGTTTGGCCTAACTCTGCTGCATGTG TCTGGTTCACAGTACCTCTCTGTACACTTTATGCCTCGCTCTATACTGTGCCTTACTGGGTCTTCTTCAT ACCTGCTTTCGTTTAGTCGTGATTCCAATAGTTAAGCTTGTTGCCTAGGACCTAGTACTCACTTGCCTCT TAAGCACAGCTGAGGACAAACCTCTTGGTGCCTTGTTGTCCCTTATTGCAACAGTGCATACTACCTGCTG CCATACAAACAACACCCATGCACCCTGTCTCTCTTCACCTTAGGCACCTGTCATTTCCAGAATGTGTCTG TGGTCAGGATAACCTCCTTACCGTCTCATGTTTTTTTAATTTTTCCAATTTCCTCAGGGTATAAATTTCC TGTAATCTTGAGATGGTCTGTAACGTAGGTTTGCTTGGGAATCACTGTATAGCCTAGGCTGACCAACTCA ATGGCTGTCCTGCCCACAGGCATGAACCTGTGGGGCTCCTAACCTTTTATTAGAGGCTATTGCAACGCCC TTACCCCTTGTACCTCAGCATCAAAAGGCTGATCGTGGCAACTCAGACCTCTGCTCATGACTGCCTCCAT TCATTTACTCACTCTCATTGCCTTAGTTCACAGATCCTTCAGTAGCCCAGGTACTCACTAAACTTCGAGC TTCTGTGAACTGTGTCTGTCCTCGCTTGTTGGAAGGCTTTTGCTTCTGGCTATACACTGCTAGCATTAAC CCAGCCCCTGCTGAATACCATTGAAATTGACCTCTCAGCTCAGTATTGCCCCTGCTGGTCATTTGCTCCA TCCCACAGTGGAACTCTTCAACCCTGTTGCCCACTCCTAATGTGTGTGTGTGTGTGTGTGTGTGTGTGTG TGTGTGTGTGTTTTCCGTGTGCACTTGTACTAATGGAACTTCTACTTTGGAGCCTTTCTGAGGGATTTTG TTTTTCTGTTGTGGGTAGTTGGTTGAGTGTGGAGGCATGTATTAGCTTCATATGCTATATAGAATCTCTT ATTTTTGAACAAATCTTATGATCATACCCTCTCTGGCTTTCATTTATGTCCGTCATGTTTGCTTTTTGTG ATAGTAAATGTATACTTGGGTATACACATGTATAAAATACAGATGTATATACATGGAAAAATAAACTAGA TGGGTTAAAGATGAACCTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4038046 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4038046 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14401 same embryo
	EMAGE:14400 same embryo
	EMAGE:14397 same embryo
	EMAGE:14396 same embryo
	EMAGE:14399 same embryo
	EurExpress:euxassay_004240 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source