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EMAGE:1440

Nkx6-2 NK6 homeobox 2 ( MGI:1352738)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:1440
0326b.jpg This embryo was treated with ProteinaseK for 30min.

Expression pattern clarity: no stars
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
central nervous system
possible possible
Authors stated that in some instances they could not determine expression levels with certainty.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3506156
Entity Detected:Nkx6-2, NK6 homeobox 2 ( MGI:1352738)
Sequence:sense strand is shown

>MGI:3506156
TGCGTTTGTGCTGAGCAGCGCGCCTTTAGCCGCGCTGCACAACATGGCTGAGATGAAGACGTCGCTGTTC
CCCTACGCGCTGCAGGGCCCGGCGGGCTTCAAGACACCCGCCCTAGGCAGCCTTGGCGCGCAGTTGCCTC
TAGGCACTCCGCACGGCATCAGCGACATCCTGGGACGGCCGGTGGGCGCAGCGGGTGGCGGCCTCCTAGG
AAGTCTGCCCCGTCTCAACGGGCTCGCCTCGTCTGCAGGTGTCTACTTCGGGCCCGCAGCCGCCGTGGCT
CGGGGCTACCCCAAGCCGCTGGCGGAACTGCCTGGGCGCCCGCCCATCTTCTGGCCTGGGGTGGTGCAGG
GCTCTCCCTGGAGGGACCCGCGACTGGCCGGCTCCGCCCAAGCCGGCGGGGTCCTGGATAAGGATGGCAA
GAAGAAACACTCGCGGCCGACTTTCTCCGGCCAGCAGATCTTCGCGCTGGAGAAGACTTTCGAGCAGACC
AAGTATTTGGCAGGCCCAGAGCGCGCGCGGCTTGCCTACTCTCTGGGCATGACCGAGAGCCAAGTGAAGG
TGTGGTTCCAGAATCGGCGGACCAAGTGGCGCAAGCGGCACGCGGCAGAGATGGCGTCGGCTAAAAAGAA
GCAAGACTCGGATGCCGAGAAGCTGAAGGTGGGTGGCTCAGACGCGGAGGACGATGACGAATACAACCGG
CCCCTGGACCCCAACTCCGATGAC
nt 217 - nt 940 of L08074.1
Notes:The template DNA sequence was PCR amplified from E13.5 or P0 mouse brain, embryonic kidney or testis cDNA using: 5' primer: ccggaattctgcgtttgtgctgagcagcg 3'primer: cccaagcttgtcatcggagttggggtccagg
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Yu J., Tenzen T., Gray P.A., Fu H., Luo P., Zhao Q., Ferrari A., Yuk D.I., Tsung E.F., Cai Z., Alberta J.A., Cheng L.P., Liu Y., Stenman J.M., Valerius M.T., Billings N., Kim H.A., Greenberg M.E., Rowitch D.H., Stiles C.D., Ma Q., McMahon A.P. Indexed by GXD, Spatially mapped by EMAGE.
Principal investigator:Andrew P. McMahon, Molecular and Cellular Biology, Harvard, Department of Molecular and Cellular Biology Harvard University 16 Divinity Ave Room 1059, Cambridge, U.S.A MA 02138
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1126/science.1104935] [ PMID:15618518] Gray PA, 2004 Mouse brain organization revealed through direct genome-scale TF expression analysis. Science (306):2255-7
Acknowledgments:This work was supported by the Charles Dana Foundation, the National Institute of Neurological Disorders and Stroke (QM, CDS, DHR & APM), the National Institute of Dental and Craniofacial Research (QM), the National Institute of Diabetes and Digestive and Kidney Diseases (APM), a Ford Foundation Postdoctoral Fellowship for Minorities (PAG), a Parker B. Francis Fellowship in Pulmonary Medicine (PAG) and the Pew Trust (QM).
Links:MGI:3508856 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE