EMAGE mouse gene expression spatial database

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EMAGE:14401

Mtmr14 myotubularin related protein 14 ( MGI:1916075)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004211. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004211_01	euxassay_004211_02	euxassay_004211_03	euxassay_004211_04

euxassay_004211_05	euxassay_004211_06	euxassay_004211_07	euxassay_004211_08	euxassay_004211_09

euxassay_004211_10	euxassay_004211_11	euxassay_004211_12	euxassay_004211_13	euxassay_004211_14

euxassay_004211_15	euxassay_004211_16	euxassay_004211_17	euxassay_004211_18	euxassay_004211_19

euxassay_004211_20	euxassay_004211_21	euxassay_004211_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14401_wholemount_strong.wlz

14401_wholemount_moderate.wlz

14401_wholemount_weak.wlz

14401_wholemount_possible.wlz

14401_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14401_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7146
Entity Detected:	Mtmr14, myotubularin related protein 14 ( MGI:1916075)
Sequence:	sense strand is shown >T7146 TTTTAGAGCCGGAGTCCGAGCTTCAGGTGCCATGGGCTGGGGGAAGGCGCCCTGAGGGGACTCGAGGCTG TGCATGGCGGGTGCTCGGGCCGCCGCCGCCGCCTCGGCGGGTTCCACCGCCTCGTCGGGCAGCCCGCCGC CTCAGGAGCCCGGGCTCTGGGAGCTGCTGGAGGAGTTCTCCCGGACTCAGTACCGTGCCAAGGACAGCGG CGGGAAGAGCGGCTCTAAGGTTGAACGCATTGAGAAGAGATGTCTGGAGCTATTTGGCCGTGACTACTGT TTCAGCGTGATTCCAAATGTGAATGGTGATATCTGCGGCCACTACCCCCGCCACATCGTATTCCTGGAGT ATGAGAGTTCTGAGAAAGAGAAAGACACATTTCAGAGCACTGTGCAGGTGAACAAGTTGCAAGACCTCAT TCACCGAAGCAAGATGGCCAGGTGCAGAGGTCGGTTTGTCTGCCCAGTGATCCTGTTCAAAGGCAAGCAT ATTTGCAGGTCGGCCACACTGGCTGGATGGGGAGAGCTATATGGACGCTCGGGATACAACTATTTATTCT CAGGGGGAGCAGATGATACCTGGGCAAGTACAGAAGATGTCACAGAAGAGGACTTTGTTCTTCGGAGTGG TGACACACACCTTTTTGATAAGGTGAGAGGCTATGATATCAAGCTGCTTCAATACCTGTCAGTCAAGTAC ATCTGTGACCTGATGGTGGAGAACAAGAAGGTGAAGTTTGGCATGAATGTAACCTCCTCTGAGAAGGTGG ACAAAGCCCAGCGCTATGCAAACTTCACTCTCCTCTCTATTCCATATCCAGGTTGTGAATTCTTCAAGGA ATATAAAGATCGGGATTACATGGCTGAAGGGCTCATATTTAACTGGAAGCAGGACTACGTCGATGCCCCA TTGAACATCCCCAACTTCCTGACTCAATCTCTGAACATTGACTGGAGCCAGTATCAGAGTTGGGACCTGG TGCAACAAACACAGAACTACCTGAAACTGCTGCTTTTCATCATGAACAGAGATGATGACAGCGGGCTGCT GGTACACTGTATCTCAGGCTGGGACCGGACCCCCCTCTTCATCTCTCTTCTGCGCCTTTCCTTGTGGGCT GATGGGCTAATTCACACATCCCTGAAGCCTGCTGAGATCCTCTACCTGACTGTGGCCTATGACTGGTTCC TCTTTGGACACATGTTGGTAGATCGGCTCAGCAAAGGGGAGGAGATTTTCTTCTTTTGCTTCAATTTTTT GAAGCATATCACCTCCGAAGAGTTCTGCCTGAAGACCCAGAGGAGGAAGAGTTTGCCAACTCGGGATGCA GGCTTCACTGTGGAAGATATCTGCATGTTGAGACACAAAGATCGAGGCAGCACAACCAGCCTAGGCAGCG ACTTCTCCCTGGTCTTGGAGCACTCACCGGGAGCTGTGGGGAGCTTCAGCTATGAGACTGTGGAACTTGC CCCGGCAGGAGCACCAACTCAGGCAGCTTGGAGGAAGAGTCACTCATCCTCCCCACAGAGTATGCTCTGG AGCCGGCCACAGCCCTCTGAGGAGCGCCTACCTTCACACCATGGGCTCACTGAAGCCAAGTCTTCTAGCT CTTCATCCTCAAACCATTCTGACAACTTTTTCAGGATGGGCAGCAGTCCTCTTGAGGTCCCCAAACCCAG ATCAGTGGACCATCCCCTGCCCGGATCCTCTCTCTCCACAGACTTTGGCAGCTGGCAGCTGGTATCGGGC TGTGGCAGTATTCAGGACCGGCCTGTCCTGCACACAGACTCCTCTCTCCCTTTCAGCTTCCAGGATGAGC TCCCTAACAGTTGTCTACTTACAGCTCTGAGTGACCGGGAGACAAGGCTGCAGGAGGTGCGGTCTGCCTT CTTGGCTGCATACAGCAGCACAGTGGGACTTCGAGCAGCAACCCCCAGTCCCTCTGGTGCCATCGGGGGC CTGCTAGAACAGTTTGCCCGTGGTGTTGGGCTCCGGGGCACAAGCACCAGCACCTTGTGAAGCTGACAGC CCACAGCCATTTCCCGCCCCTGTCTCAGCTGAGCCCTGTGGAATCAGGCCAGGCCTGGCAAAAGGGGCCC TGGAGAGCAGGGGAAATTTCCCATCCCCCTTCTCTGTTGCACACATGGCAGCCCCAAACCTGAGCAGGCC TGAGGATGGGACTTTCTACCTCCCAACCTCCTGACTGGTGACCACTCTCGTCACTCACTGCCTTCCACTG ACCCTGGTGTTCAGATTACTGTCCACTCTCAGAATTGTGTTGGAGGGTACAGGGGCGTTCCCAGGGCCTC ATGGAGATTATAATGTCAGCAAAACCCCATTCTGCAATTTTTGTTTCCTGGGAAGGAGAATCACCTGCGC TGAAAATGAGGCAATTTCACACTAATCACAAAATAAAAGCAAGTTTTTTGAATGGCAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4021243 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4021243 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14400 same embryo
	EMAGE:14398 same embryo
	EMAGE:14397 same embryo
	EMAGE:14396 same embryo
	EMAGE:14399 same embryo
	EurExpress:euxassay_004211 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source