EMAGE mouse gene expression spatial database

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EMAGE:14409

Rhobtb3 Rho-related BTB domain containing 3 ( MGI:1920546)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004272. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004272_01	euxassay_004272_02	euxassay_004272_03	euxassay_004272_04

euxassay_004272_05	euxassay_004272_06	euxassay_004272_07	euxassay_004272_08	euxassay_004272_09

euxassay_004272_10	euxassay_004272_11	euxassay_004272_12	euxassay_004272_13	euxassay_004272_14

euxassay_004272_15	euxassay_004272_16	euxassay_004272_17	euxassay_004272_18	euxassay_004272_19

euxassay_004272_20	euxassay_004272_21	euxassay_004272_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

EMAGE:14409

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14409_wholemount_strong.wlz

14409_wholemount_moderate.wlz

14409_wholemount_weak.wlz

14409_wholemount_possible.wlz

14409_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14409_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

body cavity or lining

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

limb

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

vertebral axis musculature

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

gland

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

alimentary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

integumental system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

sensory organ system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

cardiovascular system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

urinary system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

reproductive system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

respiratory system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

skeleton

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

tail

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7867
Entity Detected:	Rhobtb3, Rho-related BTB domain containing 3 ( MGI:1920546)
Sequence:	sense strand is shown >T7867 CCAGCTCCCCCGCGCGCCGGACTCTCGGGGCGACACGTGCGGGCCTCCGGCCCCGCCGAGCGGAGGCGGA GCCCGGGCGGAGGCGCGGCGGCCAGGAGCGGCGACCAGGGACCGCAGCGCTGCCAGCGCCCGGCCGGCCG CTCACTCCCGGCCCGCTCCTCTGCGTCGGTAGCGCCGCGTCGTAGGAGCGGGAGGGCCGCGGCCGGGGAT GAGCTGACTGCAGTGAGAGCGTCGCCCGGGCCCCCGCCAGGCCGCCCGCTGCCATCTCCGCGCCGCCCTG CCAGCCGCGATCATGTCCATCCACATCGTGGCGTTGGGGAACGAGGGCGACACGTTCCACCAGGACAACC GGCCGTCGGGGCTCATCCGCACATACCTGGGAAGAAGCCCGCTGGTGTCGGGGGACGAGAGTAGCTTGTT GCTGAACGCGGCCAGCACGGTCGCGCGCCCGGTGTTCACTGAGTACCAGGCCAGTGCGTTCGGGAACGTC AAGCTGGTGGTGCACGACTGTCCCGTCTGGGACATTTTTGACAGTGATTGGTACACCTCCCGAAACCTAA TTGGGGGTGCCGACATCATTGTGATCAAATACAATGTAAATGACAAGTTTTCATTCCATGAAGTAAAGGA TAACTATATTCCAGTGATTAAAAGAGCATCAAACTCAGTTCCGGTAATCATTGCGGCTGTTGGTACGAGA CAAAATGAAGAATTACCTTGTACATGCCCCCTGTGTACTTCAGACCGAGGGAGCTGTGTTACCACCACAG AAGGGATCCAACTTGCCAAAGAACTGGGAGCTACCTATCTGGAACTCCACAGCCTTGATGATTTCTACAT TGGAAAGTACTTTGGAGGAGTGCTGGAGTATTTTATGATTCAAGCCTTAAATCAGAAGACAAGTGAAAAA ATGAAGAAAAGGAAAATGACCAGCTCCTTCCATGGGATTCGACCTCCTCAGCTTGAACAGCCAGAAAAGA TGCCTGTCTTGAAAGCTGAGGCATCACACTATCACTCCGACTTGAATAACTTGCTGCTCTGCTGCCAGTG TGTGGATGTGGTTTTTTACCACCCAGAAGTGACGGGCGTCGTGGAAGCCCACAAGATCGTTCTCTGCTCT GTAAGCCATGTTTTCATGCTGCTTTTCAATGTGAAAAGCCCGGCCGACATTCAGGATTCCAGTATCATCC GAACCACCCAGGATCTCTTTGCCATAAACAGAGATGCTGTGCTTCCAGGTGCTAGCCAGGAAGCCCCCAG CAACCCACCATTGCCTGTTATTGTTAAGGACGCCCTCTTCTGCTCCTGTTTATCAGACATTCTGCGCTTC ATTTATTCAGGTGCTTTTCAGTGGGAAGAGCTGGAAGAAGATGTCAGGAGGAAGTTGAAAGATTCCGGGG ATGTTTCTGATATAATCGAGAAAGTTAAATGTATTTTAAAAACACCAGGAAAGATTAATTGCCTGAGGAA CTGCAAGACCTACCAAGCCAGGAAGCCTCTGTGGTTTTATAATACGTCTCTCAAGTTTTTCCTTAATAAA CCGATGCTTGCCGATGTTGTCTTTGAAATACAAGGAGCAACTGTGCCAGCCCACAGGGCCATCTTGGTGG CACGATGTGAAGTGATGGCTGCCATGTTTAATGGTAATTATATGGAAGCCAAGAGTGTACTGATTCCAGT TTATGGCGTTTCCAAAGAGACATTCTTGTCATTTTTAGAATACTTATACACAGACTCTTGTTGCCCAGCT GGCATTTTTCAGGCCATGTGTCTCCTGATCTGTGCTGAGATGTACCAGGTCTCTAGACTGCAGCACATCT GTGAGCTGTTCATCATCACACAGCTGCAGAGCATGCCCAGCAGGGAGCTGGCCTCCATGAACCTCGACAT TGTAGACCTGCTCAAAAAGGCCAAGTTTCACCACTCAGACTGCCTTTCCACCTGGCTGCTTCATTTCATC GCCACTAACTACCTCATATTCAGCCAAAAGCCTGAATTTCAAGACCTTTCAGTGGAAGAACGAAGTTTTG TTGAAAAGCACAGATGGCCATCAAACATGTACTTGAAGCAGCTGGCAGAATACAGGAAGTATATCCACTC CCGGAAATGTCGCTGTTTAGTCATGTGACCTGAGCGTTTGCATACATACACTTTTTTTTATTATGAAGAA TGGGTACTTCTGGATTCCAGGAAAAATTCTTATAACTAAAATCAATATGTTTTAAAAGTCACCATATAGC TTCTTACTAGTTATCTTTTAAAGACTGTGGTGGTCTTAAAAGGGAACAAAAGCTACTACAGACTAAATTT ATGTTTCACCTAGAATATAAAACTGTTAGACATTGGGTTCTCCTTTACGTTGCCCTATTGCTTTAATAAC ATCAAGATCTCTCCTAGTTAAGAGGTGTTTAATTAAAAAAAAAAAGCATCTAGGATAAATATAAAAATTT CATCATGTCTGAGTGGTTGCTCTGCTAAAATAAGGGGCATTTAAAAGCCAAATGTAACCATGGGATTCAG AAGAGCACTAACCAGTGCAGCATAGAAATAGGAATAGCGTAGGAATAGAAATTTGCCCAAGACTTTGATA GTCATCTGGTTTCAGTTCAGCAAGGGTACTTGAATCAATATGGTAGCCCCATCTGGACCATCCACCCACA TCTGCTTGTCAATCTGGCTGCAAACCTAAGCAGGCAGGTAGTCTATGGACTTCCAAACTTAGAAGGGTCT GATATTAGGTTTAGTGCTCTGCTAGTGCAGTTGTTGAATTCTTAAGAATGTTTGATGAAGGCATATTTTC ACTTTGTACTGGCTCACCAAAATTGCTGAGATGGTGTTTTCTTTTCCGTTTGGTTTTGGGTTTTTTTTTG GTTTTTTTTTTTGGTTTTGGTTTTGTTTTAAGATGGGGTTTCTCTGTGTAACATTCCTAGCTGTCCTGGA ACTTGCTTTATAGTCCAGGCTGGCCTCACATCCACAGAGACATGCTAGGATTAAGGGTGTGTGCCACTAT GCCTGGCTTAAGTCTTTTTCTCACTCAAACCTTCTCTCTGTAGAAGCAGAAAAGTTGCTGATAGCCAGGA AAGAGAGCTTTCGGCCAGGGGAGCAGAGTGGAGACTCCCATCTTTGGGGAAGCATATATGCTCAAGCGCC TCTACAAAGACATTCCCTGGCAGTAAGAAATGCTGAAATGCTTTTTGTTGGTTTTGAAACACTCCCCCAG TTCTAGCTGTACCCCAGGAAAACAAATACTTTCTTGATCAATATGTAAAGCCAGGAAGTTGGTAAGAGTG ACTGAGTAGCCTGACTATAATACACAGTTATAGTGATAATCACAGGAAAAGATGCCTTCTAAAGACTTGG GCATAAAAGCTTAAATATTCTTTCTTTTAAATGTCTGTTAAGAATATGATGCCAATGGCTTGAATTGAGT TCTTTTCAAGAACTGATAGACTATTTGGTATGAAGTAGCAAAGCTACCTAGGAAGGTTAAAAAATTATGT ACATCAAAGATATCCTAGATGTAGAGACGATGGTGTCTGGGATAAAAGGCAGAGTTAGTTTCTCTCATAG CATATATTGGCAGAAATGGTTCTGCAAGATGCTCAAAGGCATGGAAGAAACATATTACTATTTATTGTTA ATATCATGGTCATCTGAAGTTAAGTACTGGGATATATTTTTCTAATTTCTAGTGATCTTAGTATGCATAT TTTTTTCTAACCTTTCAAAAGTTTGATTCAACCGTCTTACCTTGTAGTGTTAAGATTCCATACAGAGAGC CCTGTTATGCATACCAGTACTACAAAATAAAGGGGAAAGAATTCTTGTAGATAGAATCCCAAGAGAGCTC ATTTAATTGTGCTGTGACAATGAAGATTACTGGAATGAATTTTTGTCTTTTGTATTTTAATCACTTGAGT GAATCAACTATTAGCAAATTCCATTTGGTGGCTTAGCATTGGAAAGAACAGATAAAGTAGCTGATGCCTA GAAGATCCTCTGCTTTTTCCGATCCAGTGGTAGCCTTTGATACTCCAAAGTAATTATGTTCACCCTCTGT ACTAATGACATTTACAATTCTAAAATTTTATGAAATTGAAATGAAAACTAAGAGTTGTAAAACAAAGTAT TTTCTTGGGAATATTACTTTAACATTCAGATGGATCATAACTAATTCGACTAGGGTGTTTTTCCTGAGTA GGAGTTAAGTTTGGAACTGGTGGTCTTGAATGTTAAACATGGAGTGTGACTGGGACAGCCATGAGTCAGA ATATAAATGAATGCTTAAACATTTGAATACAGATTCCTCCGGATTGTTGAATTCACTCAACTAGAAGTAA TAGGCGATTTTAAATCTTTATAAATGATCTTGGGTAAATTACTGGTGATTGTGAGGGTTGTTTCCCAATC AAAGGTTATTTCTAGATGGCTATATGCTTTGTTGCAATTTCTGCTTCCACCTATTTTCCAAGCATTTTAC TTAAGAAGGATACTGTGTAGTTGAACTAAGGCTCTTTCCAGGCCGGGCTTGCATCCTGTTGTGTATTCTT TGTATGCTGCTGACTATATCCCTATTCTGCTGTAGGGGACTAGATTTCTAATAGAGAACATTGGCATGGT AGCTGAAGCCCACTCCTGTGCAAGCCATACTGGAACTTCATATTGAGAATAGAAGACGTAGAAATCAGAT GGATTTCCAAAGTGGCCAAACTTTTCAACTGTAATTTTATAAATGATACTACACTGATATAGCCAATTAG ATTTAAAATGTCTTCAAAAATTACTTTATTTTATTTAAAGCATCTGTTTAATGCAACTTTTAATCAGATT TTGCAAAGGGTATGTATGTATTTTAATATAGTCTGACCTGAATTTATATGTTTTTAGCTTTAGTATTTAA CCTTTTATAACAAATAAACCATTTTTTAAAAAAACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6394317 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6394317 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14408 same embryo
	EMAGE:14407 same embryo
	EMAGE:14412 same embryo
	EMAGE:14411 same embryo
	EMAGE:14410 same embryo
	EurExpress:euxassay_004272 same experiment
	MGI:4827733 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source